Identification of a Novel Mutation Exacerbated the PSI Photoinhibition in pgr5/pgrl1 Mutants; Caution for Overestimation of the Phenotypes in Arabidopsis pgr5-1 Mutant

PSI photoinhibition is usually avoided through P700 oxidation. Without this protective mechanism, excess light represents a potentially lethal threat to plants. PGR5 is suggested to be a major component of cyclic electron transport around PSI and is important for P700 oxidation in angiosperms. The k...

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Autores principales:	Shinya Wada, Katsumi Amako, Chikahiro Miyake
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	proton gradient regulation 5 (PGR5) PGR5-like photosynthetic phenotype 1 (PGRL1) photosynthetic electron transport PSI photoinhibition oxidation of P700 oxidative stress Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/71ec2f81f50742fc99214440ab9e88d2
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spelling	oai:doaj.org-article:71ec2f81f50742fc99214440ab9e88d22021-11-25T17:08:31ZIdentification of a Novel Mutation Exacerbated the PSI Photoinhibition in <i>pgr5</i>/<i>pgrl1</i> Mutants; Caution for Overestimation of the Phenotypes in Arabidopsis <i>pgr5-1</i> Mutant10.3390/cells101128842073-4409https://doaj.org/article/71ec2f81f50742fc99214440ab9e88d22021-10-01T00:00:00Zhttps://www.mdpi.com/2073-4409/10/11/2884https://doaj.org/toc/2073-4409PSI photoinhibition is usually avoided through P700 oxidation. Without this protective mechanism, excess light represents a potentially lethal threat to plants. PGR5 is suggested to be a major component of cyclic electron transport around PSI and is important for P700 oxidation in angiosperms. The known Arabidopsis PGR5 deficient mutant, <i>pgr5-1</i>, is incapable of P700 oxidation regulation and has been used in numerous photosynthetic studies. However, here it was revealed that <i>pgr5-1</i> was a double mutant with exaggerated PSI photoinhibition. <i>pgr5-1</i> significantly reduced growth compared to the newly isolated PGR5 deficient mutant, <i>pgr5<sup>hope1</sup></i>. The introduction of PGR5 into <i>pgr5-1</i> restored P700 oxidation regulation, but remained a pale-green phenotype, indicating that <i>pgr5-1</i> had additional mutations. Both <i>pgr5-1</i> and <i>pgr5<sup>hope1</sup></i> tended to cause PSI photoinhibition by excess light, but <i>pgr5-1</i> exhibited an enhanced reduction in PSI activity. Introducing AT2G17240, a candidate gene for the second mutation into <i>pgr5-1</i> restored the pale-green phenotype and partially restored PSI activity. Furthermore, a deficient mutant of PGRL1 complexing with PGR5 significantly reduced PSI activity in the double-deficient mutant with AT2G17240. From these results, we concluded that AT2G17240, named PSI photoprotection 1 (PTP1), played a role in PSI photoprotection, especially in PGR5/PGRL1 deficient mutants.Shinya WadaKatsumi AmakoChikahiro MiyakeMDPI AGarticleproton gradient regulation 5 (PGR5)PGR5-like photosynthetic phenotype 1 (PGRL1)photosynthetic electron transportPSI photoinhibitionoxidation of P700oxidative stressBiology (General)QH301-705.5ENCells, Vol 10, Iss 2884, p 2884 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	proton gradient regulation 5 (PGR5) PGR5-like photosynthetic phenotype 1 (PGRL1) photosynthetic electron transport PSI photoinhibition oxidation of P700 oxidative stress Biology (General) QH301-705.5
spellingShingle	proton gradient regulation 5 (PGR5) PGR5-like photosynthetic phenotype 1 (PGRL1) photosynthetic electron transport PSI photoinhibition oxidation of P700 oxidative stress Biology (General) QH301-705.5 Shinya Wada Katsumi Amako Chikahiro Miyake Identification of a Novel Mutation Exacerbated the PSI Photoinhibition in <i>pgr5</i>/<i>pgrl1</i> Mutants; Caution for Overestimation of the Phenotypes in Arabidopsis <i>pgr5-1</i> Mutant
description	PSI photoinhibition is usually avoided through P700 oxidation. Without this protective mechanism, excess light represents a potentially lethal threat to plants. PGR5 is suggested to be a major component of cyclic electron transport around PSI and is important for P700 oxidation in angiosperms. The known Arabidopsis PGR5 deficient mutant, <i>pgr5-1</i>, is incapable of P700 oxidation regulation and has been used in numerous photosynthetic studies. However, here it was revealed that <i>pgr5-1</i> was a double mutant with exaggerated PSI photoinhibition. <i>pgr5-1</i> significantly reduced growth compared to the newly isolated PGR5 deficient mutant, <i>pgr5<sup>hope1</sup></i>. The introduction of PGR5 into <i>pgr5-1</i> restored P700 oxidation regulation, but remained a pale-green phenotype, indicating that <i>pgr5-1</i> had additional mutations. Both <i>pgr5-1</i> and <i>pgr5<sup>hope1</sup></i> tended to cause PSI photoinhibition by excess light, but <i>pgr5-1</i> exhibited an enhanced reduction in PSI activity. Introducing AT2G17240, a candidate gene for the second mutation into <i>pgr5-1</i> restored the pale-green phenotype and partially restored PSI activity. Furthermore, a deficient mutant of PGRL1 complexing with PGR5 significantly reduced PSI activity in the double-deficient mutant with AT2G17240. From these results, we concluded that AT2G17240, named PSI photoprotection 1 (PTP1), played a role in PSI photoprotection, especially in PGR5/PGRL1 deficient mutants.
format	article
author	Shinya Wada Katsumi Amako Chikahiro Miyake
author_facet	Shinya Wada Katsumi Amako Chikahiro Miyake
author_sort	Shinya Wada
title	Identification of a Novel Mutation Exacerbated the PSI Photoinhibition in <i>pgr5</i>/<i>pgrl1</i> Mutants; Caution for Overestimation of the Phenotypes in Arabidopsis <i>pgr5-1</i> Mutant
title_short	Identification of a Novel Mutation Exacerbated the PSI Photoinhibition in <i>pgr5</i>/<i>pgrl1</i> Mutants; Caution for Overestimation of the Phenotypes in Arabidopsis <i>pgr5-1</i> Mutant
title_full	Identification of a Novel Mutation Exacerbated the PSI Photoinhibition in <i>pgr5</i>/<i>pgrl1</i> Mutants; Caution for Overestimation of the Phenotypes in Arabidopsis <i>pgr5-1</i> Mutant
title_fullStr	Identification of a Novel Mutation Exacerbated the PSI Photoinhibition in <i>pgr5</i>/<i>pgrl1</i> Mutants; Caution for Overestimation of the Phenotypes in Arabidopsis <i>pgr5-1</i> Mutant
title_full_unstemmed	Identification of a Novel Mutation Exacerbated the PSI Photoinhibition in <i>pgr5</i>/<i>pgrl1</i> Mutants; Caution for Overestimation of the Phenotypes in Arabidopsis <i>pgr5-1</i> Mutant
title_sort	identification of a novel mutation exacerbated the psi photoinhibition in <i>pgr5</i>/<i>pgrl1</i> mutants; caution for overestimation of the phenotypes in arabidopsis <i>pgr5-1</i> mutant
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/71ec2f81f50742fc99214440ab9e88d2
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Identification of a Novel Mutation Exacerbated the PSI Photoinhibition in <i>pgr5</i>/<i>pgrl1</i> Mutants; Caution for Overestimation of the Phenotypes in Arabidopsis <i>pgr5-1</i> Mutant

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