A Gelatin-sulfonated Silk Composite Scaffold based on 3D Printing Technology Enhances Skin Regeneration by Stimulating Epidermal Growth and Dermal Neovascularization

Abstract One of the key problems hindering skin repair is the deficiency of dermal vascularization and difficulty of epidermis regeneration, which makes it challenging to fabricate scaffolds that can biologically fulfill the requirements for skin regeneration. To overcome this problem, three-dimensi...

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Autores principales:	Si Xiong, Xianzhu Zhang, Ping Lu, Yan Wu, Quan Wang, Heng Sun, Boon Chin Heng, Varitsara Bunpetch, Shufang Zhang, Hongwei Ouyang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/71ecfe4f4deb401ba4b714fed063af9a
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spelling	oai:doaj.org-article:71ecfe4f4deb401ba4b714fed063af9a2021-12-02T15:06:19ZA Gelatin-sulfonated Silk Composite Scaffold based on 3D Printing Technology Enhances Skin Regeneration by Stimulating Epidermal Growth and Dermal Neovascularization10.1038/s41598-017-04149-y2045-2322https://doaj.org/article/71ecfe4f4deb401ba4b714fed063af9a2017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04149-yhttps://doaj.org/toc/2045-2322Abstract One of the key problems hindering skin repair is the deficiency of dermal vascularization and difficulty of epidermis regeneration, which makes it challenging to fabricate scaffolds that can biologically fulfill the requirements for skin regeneration. To overcome this problem, three-dimensional printing was used to fabricate a gelatin-sulfonated silk composite scaffold that was incorporated with basic fibroblast growth factor 2 (FGF-2) through binding with a sulfonic acid group (SO3) (3DG-SF-SO3-FGF). The efficacy and mechanism by which the 3DG-SF-SO3-FGF scaffolds promote skin regeneration were investigated both within in vitro cell culture and in vivo with a full-thickness skin defect model. The histological results showed that the gelatin-sulfonated silk composite scaffolds promoted granulation, and that incorporation of FGF-2 significantly enhanced the regeneration of skin-like tissues after implantation in rat skin defects for 14 and 28 days. Further investigations demonstrated that 3DG-SF-SO3-FGF scaffolds might stimulate dermal vascularization. These findings thus suggest that incorporation of FGF-2 into the 3D printed scaffolds is a viable strategy for enhancing skin regeneration.Si XiongXianzhu ZhangPing LuYan WuQuan WangHeng SunBoon Chin HengVaritsara BunpetchShufang ZhangHongwei OuyangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Si Xiong Xianzhu Zhang Ping Lu Yan Wu Quan Wang Heng Sun Boon Chin Heng Varitsara Bunpetch Shufang Zhang Hongwei Ouyang A Gelatin-sulfonated Silk Composite Scaffold based on 3D Printing Technology Enhances Skin Regeneration by Stimulating Epidermal Growth and Dermal Neovascularization
description	Abstract One of the key problems hindering skin repair is the deficiency of dermal vascularization and difficulty of epidermis regeneration, which makes it challenging to fabricate scaffolds that can biologically fulfill the requirements for skin regeneration. To overcome this problem, three-dimensional printing was used to fabricate a gelatin-sulfonated silk composite scaffold that was incorporated with basic fibroblast growth factor 2 (FGF-2) through binding with a sulfonic acid group (SO3) (3DG-SF-SO3-FGF). The efficacy and mechanism by which the 3DG-SF-SO3-FGF scaffolds promote skin regeneration were investigated both within in vitro cell culture and in vivo with a full-thickness skin defect model. The histological results showed that the gelatin-sulfonated silk composite scaffolds promoted granulation, and that incorporation of FGF-2 significantly enhanced the regeneration of skin-like tissues after implantation in rat skin defects for 14 and 28 days. Further investigations demonstrated that 3DG-SF-SO3-FGF scaffolds might stimulate dermal vascularization. These findings thus suggest that incorporation of FGF-2 into the 3D printed scaffolds is a viable strategy for enhancing skin regeneration.
format	article
author	Si Xiong Xianzhu Zhang Ping Lu Yan Wu Quan Wang Heng Sun Boon Chin Heng Varitsara Bunpetch Shufang Zhang Hongwei Ouyang
author_facet	Si Xiong Xianzhu Zhang Ping Lu Yan Wu Quan Wang Heng Sun Boon Chin Heng Varitsara Bunpetch Shufang Zhang Hongwei Ouyang
author_sort	Si Xiong
title	A Gelatin-sulfonated Silk Composite Scaffold based on 3D Printing Technology Enhances Skin Regeneration by Stimulating Epidermal Growth and Dermal Neovascularization
title_short	A Gelatin-sulfonated Silk Composite Scaffold based on 3D Printing Technology Enhances Skin Regeneration by Stimulating Epidermal Growth and Dermal Neovascularization
title_full	A Gelatin-sulfonated Silk Composite Scaffold based on 3D Printing Technology Enhances Skin Regeneration by Stimulating Epidermal Growth and Dermal Neovascularization
title_fullStr	A Gelatin-sulfonated Silk Composite Scaffold based on 3D Printing Technology Enhances Skin Regeneration by Stimulating Epidermal Growth and Dermal Neovascularization
title_full_unstemmed	A Gelatin-sulfonated Silk Composite Scaffold based on 3D Printing Technology Enhances Skin Regeneration by Stimulating Epidermal Growth and Dermal Neovascularization
title_sort	gelatin-sulfonated silk composite scaffold based on 3d printing technology enhances skin regeneration by stimulating epidermal growth and dermal neovascularization
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/71ecfe4f4deb401ba4b714fed063af9a
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A Gelatin-sulfonated Silk Composite Scaffold based on 3D Printing Technology Enhances Skin Regeneration by Stimulating Epidermal Growth and Dermal Neovascularization

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