Standardization of esophageal adenocarcinoma in vitro model and its applicability for model drug testing

Abstract FLO-1 cell line represents an important tool in esophageal adenocarcinoma (EAC) research as a verified and authentic cell line to study the disease pathophysiology and antitumor drug screenings. Since in vitro characteristics of cells depend on the microenvironment and culturing conditions,...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Larisa Tratnjek, Nadica Sibinovska, Slavko Kralj, Darko Makovec, Katja Kristan, Mateja Erdani Kreft
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/71ed6df8a9fa45b7b60a7a4a76dac028
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:71ed6df8a9fa45b7b60a7a4a76dac028
record_format	dspace
spelling	oai:doaj.org-article:71ed6df8a9fa45b7b60a7a4a76dac0282021-12-02T13:24:25ZStandardization of esophageal adenocarcinoma in vitro model and its applicability for model drug testing10.1038/s41598-021-85530-w2045-2322https://doaj.org/article/71ed6df8a9fa45b7b60a7a4a76dac0282021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-85530-whttps://doaj.org/toc/2045-2322Abstract FLO-1 cell line represents an important tool in esophageal adenocarcinoma (EAC) research as a verified and authentic cell line to study the disease pathophysiology and antitumor drug screenings. Since in vitro characteristics of cells depend on the microenvironment and culturing conditions, we performed a thorough characterization of the FLO-1 cell line under different culturing conditions with the aim of (1) examining the effect of serum-free growth medium and air–liquid interface (A–L) culturing, which better reflect physiological conditions in vivo and (2) investigating the differentiation potential of FLO-1 cells to mimic the properties of the in vivo esophageal epithelium. Our study shows that the composition of the media influenced the morphological, ultrastructural and molecular characteristics of FLO-1 cells, such as the expression of junctional proteins. Importantly, FLO-1 cells formed spheres at the A–L interface, recapitulating key elements of tumors in the esophageal tube, i.e., direct contact with the gas phase and three-dimensional architecture. On the other hand, FLO-1 models exhibited high permeability to model drugs and zero permeability markers, and low transepithelial resistance, and therefore poorly mimicked normal esophageal epithelium. In conclusion, the identified effect of culture conditions on the characteristics of FLO-1 cells should be considered for standardization, data reproducibility and validity of the in vitro EAC model. Moreover, the sphere-forming ability of FLO-1 cells at the A–L interface should be considered in EAC tumor biology and anticancer drug studies as a reliable and straightforward model with the potential to increase the predictive efficiency of the current in vitro approaches.Larisa TratnjekNadica SibinovskaSlavko KraljDarko MakovecKatja KristanMateja Erdani KreftNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-23 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Larisa Tratnjek Nadica Sibinovska Slavko Kralj Darko Makovec Katja Kristan Mateja Erdani Kreft Standardization of esophageal adenocarcinoma in vitro model and its applicability for model drug testing
description	Abstract FLO-1 cell line represents an important tool in esophageal adenocarcinoma (EAC) research as a verified and authentic cell line to study the disease pathophysiology and antitumor drug screenings. Since in vitro characteristics of cells depend on the microenvironment and culturing conditions, we performed a thorough characterization of the FLO-1 cell line under different culturing conditions with the aim of (1) examining the effect of serum-free growth medium and air–liquid interface (A–L) culturing, which better reflect physiological conditions in vivo and (2) investigating the differentiation potential of FLO-1 cells to mimic the properties of the in vivo esophageal epithelium. Our study shows that the composition of the media influenced the morphological, ultrastructural and molecular characteristics of FLO-1 cells, such as the expression of junctional proteins. Importantly, FLO-1 cells formed spheres at the A–L interface, recapitulating key elements of tumors in the esophageal tube, i.e., direct contact with the gas phase and three-dimensional architecture. On the other hand, FLO-1 models exhibited high permeability to model drugs and zero permeability markers, and low transepithelial resistance, and therefore poorly mimicked normal esophageal epithelium. In conclusion, the identified effect of culture conditions on the characteristics of FLO-1 cells should be considered for standardization, data reproducibility and validity of the in vitro EAC model. Moreover, the sphere-forming ability of FLO-1 cells at the A–L interface should be considered in EAC tumor biology and anticancer drug studies as a reliable and straightforward model with the potential to increase the predictive efficiency of the current in vitro approaches.
format	article
author	Larisa Tratnjek Nadica Sibinovska Slavko Kralj Darko Makovec Katja Kristan Mateja Erdani Kreft
author_facet	Larisa Tratnjek Nadica Sibinovska Slavko Kralj Darko Makovec Katja Kristan Mateja Erdani Kreft
author_sort	Larisa Tratnjek
title	Standardization of esophageal adenocarcinoma in vitro model and its applicability for model drug testing
title_short	Standardization of esophageal adenocarcinoma in vitro model and its applicability for model drug testing
title_full	Standardization of esophageal adenocarcinoma in vitro model and its applicability for model drug testing
title_fullStr	Standardization of esophageal adenocarcinoma in vitro model and its applicability for model drug testing
title_full_unstemmed	Standardization of esophageal adenocarcinoma in vitro model and its applicability for model drug testing
title_sort	standardization of esophageal adenocarcinoma in vitro model and its applicability for model drug testing
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/71ed6df8a9fa45b7b60a7a4a76dac028
work_keys_str_mv	AT larisatratnjek standardizationofesophagealadenocarcinomainvitromodelanditsapplicabilityformodeldrugtesting AT nadicasibinovska standardizationofesophagealadenocarcinomainvitromodelanditsapplicabilityformodeldrugtesting AT slavkokralj standardizationofesophagealadenocarcinomainvitromodelanditsapplicabilityformodeldrugtesting AT darkomakovec standardizationofesophagealadenocarcinomainvitromodelanditsapplicabilityformodeldrugtesting AT katjakristan standardizationofesophagealadenocarcinomainvitromodelanditsapplicabilityformodeldrugtesting AT matejaerdanikreft standardizationofesophagealadenocarcinomainvitromodelanditsapplicabilityformodeldrugtesting
_version_	1718393100810846208

Standardization of esophageal adenocarcinoma in vitro model and its applicability for model drug testing

Ejemplares similares