Sex Differences in Early Programming by Maternal High Fat Diet Induced-Obesity and Fish Oil Supplementation in Mice

Pre-pregnancy obesity is a contributing factor for impairments in offspring metabolic health. Interventional strategies during pregnancy are a potential approach to alleviate and/or prevent obesity and obesity related metabolic alterations in the offspring. Fish oil (FO), rich in omega-3 polyunsatur...

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Autores principales:	Latha Ramalingam, Kalhara R. Menikdiwela, Stephani Spainhour, Tochi Eboh, Naima Moustaid-Moussa
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	maternal obesity high fat diet fish oil sex differences mice Nutrition. Foods and food supply TX341-641
Acceso en línea:	https://doaj.org/article/71f895c2e8c14116b05efd0418a6b948
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spelling	oai:doaj.org-article:71f895c2e8c14116b05efd0418a6b9482021-11-25T18:33:17ZSex Differences in Early Programming by Maternal High Fat Diet Induced-Obesity and Fish Oil Supplementation in Mice10.3390/nu131137032072-6643https://doaj.org/article/71f895c2e8c14116b05efd0418a6b9482021-10-01T00:00:00Zhttps://www.mdpi.com/2072-6643/13/11/3703https://doaj.org/toc/2072-6643Pre-pregnancy obesity is a contributing factor for impairments in offspring metabolic health. Interventional strategies during pregnancy are a potential approach to alleviate and/or prevent obesity and obesity related metabolic alterations in the offspring. Fish oil (FO), rich in omega-3 polyunsaturated fatty acids (<i>n</i>-3 PUFAs) exerts metabolic health benefits. However, the role of FO in early life remains still unknown. Hence, this study objective was to determine the effect of FO supplementation in mice from pre-pregnancy through lactation, and to study the post-natal metabolic health effects in gonadal fat and liver of offspring fed high fat (HF) diet with or without FO. Female C57BL6J mice aged 4–5 weeks were fed a HF (45% fat) diet supplemented with or without FO (30 g/kg of diet) and low fat (LF; 10% fat) pre-pregnancy through lactation. After weaning, offspring (male and female) from HF or FO dams either continued the same diet (HF-HF and FO-FO) or switched to the other diet (HF-FO and FO-HF) for 13 weeks, creating four groups of treatment, and LF-LF was used as a control group. Serum, gonadal fat and liver tissue were collected at termination for metabolic analyses. Offspring of both sexes fed HF with or without fish oil gained (<i>p</i> < 0.05) more weight post weaning, compared to LF-LF-fed mice. All the female offspring groups supplemented with FO had reduced body weight compared to the respective male groups. Further, FO-FO supplementation in both sexes (<i>p</i> < 0.05) improved glucose clearance and insulin sensitivity compared to HF-HF. All FO-FO fed mice had significantly reduced adipocyte size compared to HF-HF group in both male and females. Inflammation, measured by mRNA levels of monocyte chemoattractant protein 1 <i>(Mcp1)</i>, was reduced (<i>p</i> < 0.05) with FO supplementation in both sexes in gonadal fat and in the liver. Markers of fatty acid synthesis, fatty acid synthase (<i>Fasn</i>) showed no sex specific differences in gonadal fat and liver of mice supplemented with HF. Female mice had lower liver triglycerides than male counterparts. Supplementation of FO in mice improved metabolic health of offspring by lowering markers of lipid synthesis and inflammation.Latha RamalingamKalhara R. MenikdiwelaStephani SpainhourTochi EbohNaima Moustaid-MoussaMDPI AGarticlematernal obesityhigh fat dietfish oilsex differencesmiceNutrition. Foods and food supplyTX341-641ENNutrients, Vol 13, Iss 3703, p 3703 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	maternal obesity high fat diet fish oil sex differences mice Nutrition. Foods and food supply TX341-641
spellingShingle	maternal obesity high fat diet fish oil sex differences mice Nutrition. Foods and food supply TX341-641 Latha Ramalingam Kalhara R. Menikdiwela Stephani Spainhour Tochi Eboh Naima Moustaid-Moussa Sex Differences in Early Programming by Maternal High Fat Diet Induced-Obesity and Fish Oil Supplementation in Mice
description	Pre-pregnancy obesity is a contributing factor for impairments in offspring metabolic health. Interventional strategies during pregnancy are a potential approach to alleviate and/or prevent obesity and obesity related metabolic alterations in the offspring. Fish oil (FO), rich in omega-3 polyunsaturated fatty acids (<i>n</i>-3 PUFAs) exerts metabolic health benefits. However, the role of FO in early life remains still unknown. Hence, this study objective was to determine the effect of FO supplementation in mice from pre-pregnancy through lactation, and to study the post-natal metabolic health effects in gonadal fat and liver of offspring fed high fat (HF) diet with or without FO. Female C57BL6J mice aged 4–5 weeks were fed a HF (45% fat) diet supplemented with or without FO (30 g/kg of diet) and low fat (LF; 10% fat) pre-pregnancy through lactation. After weaning, offspring (male and female) from HF or FO dams either continued the same diet (HF-HF and FO-FO) or switched to the other diet (HF-FO and FO-HF) for 13 weeks, creating four groups of treatment, and LF-LF was used as a control group. Serum, gonadal fat and liver tissue were collected at termination for metabolic analyses. Offspring of both sexes fed HF with or without fish oil gained (<i>p</i> < 0.05) more weight post weaning, compared to LF-LF-fed mice. All the female offspring groups supplemented with FO had reduced body weight compared to the respective male groups. Further, FO-FO supplementation in both sexes (<i>p</i> < 0.05) improved glucose clearance and insulin sensitivity compared to HF-HF. All FO-FO fed mice had significantly reduced adipocyte size compared to HF-HF group in both male and females. Inflammation, measured by mRNA levels of monocyte chemoattractant protein 1 <i>(Mcp1)</i>, was reduced (<i>p</i> < 0.05) with FO supplementation in both sexes in gonadal fat and in the liver. Markers of fatty acid synthesis, fatty acid synthase (<i>Fasn</i>) showed no sex specific differences in gonadal fat and liver of mice supplemented with HF. Female mice had lower liver triglycerides than male counterparts. Supplementation of FO in mice improved metabolic health of offspring by lowering markers of lipid synthesis and inflammation.
format	article
author	Latha Ramalingam Kalhara R. Menikdiwela Stephani Spainhour Tochi Eboh Naima Moustaid-Moussa
author_facet	Latha Ramalingam Kalhara R. Menikdiwela Stephani Spainhour Tochi Eboh Naima Moustaid-Moussa
author_sort	Latha Ramalingam
title	Sex Differences in Early Programming by Maternal High Fat Diet Induced-Obesity and Fish Oil Supplementation in Mice
title_short	Sex Differences in Early Programming by Maternal High Fat Diet Induced-Obesity and Fish Oil Supplementation in Mice
title_full	Sex Differences in Early Programming by Maternal High Fat Diet Induced-Obesity and Fish Oil Supplementation in Mice
title_fullStr	Sex Differences in Early Programming by Maternal High Fat Diet Induced-Obesity and Fish Oil Supplementation in Mice
title_full_unstemmed	Sex Differences in Early Programming by Maternal High Fat Diet Induced-Obesity and Fish Oil Supplementation in Mice
title_sort	sex differences in early programming by maternal high fat diet induced-obesity and fish oil supplementation in mice
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/71f895c2e8c14116b05efd0418a6b948
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Sex Differences in Early Programming by Maternal High Fat Diet Induced-Obesity and Fish Oil Supplementation in Mice

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