Porcine familial adenomatous polyposis model enables systematic analysis of early events in adenoma progression

Abstract We compared gene expression in low and high-grade intraepithelial dysplastic polyps from pigs carrying an APC 1311 truncating mutation orthologous to human APC 1309 , analysing whole samples and microdissected dysplastic epithelium. Gene set enrichment analysis revealed differential express...

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Autores principales:	Tatiana Flisikowska, Monika Stachowiak, Hongen Xu, Alexandra Wagner, Alejandra Hernandez-Caceres, Christine Wurmser, Carolin Perleberg, Hubert Pausch, Anna Perkowska, Konrad Fischer, Dmitrij Frishman, Ruedi Fries, Marek Switonski, Alexander Kind, Dieter Saur, Angelika Schnieke, Krzysztof Flisikowski
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/72016a41c779481dadcfe7319ad01032
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spelling	oai:doaj.org-article:72016a41c779481dadcfe7319ad010322021-12-02T15:05:21ZPorcine familial adenomatous polyposis model enables systematic analysis of early events in adenoma progression10.1038/s41598-017-06741-82045-2322https://doaj.org/article/72016a41c779481dadcfe7319ad010322017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06741-8https://doaj.org/toc/2045-2322Abstract We compared gene expression in low and high-grade intraepithelial dysplastic polyps from pigs carrying an APC 1311 truncating mutation orthologous to human APC 1309 , analysing whole samples and microdissected dysplastic epithelium. Gene set enrichment analysis revealed differential expression of gene sets similar to human normal mucosa versus T1 stage polyps. Transcriptome analysis of whole samples revealed many differentially-expressed genes reflecting immune infiltration. Analysis of microdissected dysplastic epithelium was markedly different and showed increased expression in high-grade intraepithelial neoplasia of several genes known to be involved in human CRC; and revealed possible new roles for GBP6 and PLXND1. The pig model thus facilitates analysis of CRC pathogenesis.Tatiana FlisikowskaMonika StachowiakHongen XuAlexandra WagnerAlejandra Hernandez-CaceresChristine WurmserCarolin PerlebergHubert PauschAnna PerkowskaKonrad FischerDmitrij FrishmanRuedi FriesMarek SwitonskiAlexander KindDieter SaurAngelika SchniekeKrzysztof FlisikowskiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Tatiana Flisikowska Monika Stachowiak Hongen Xu Alexandra Wagner Alejandra Hernandez-Caceres Christine Wurmser Carolin Perleberg Hubert Pausch Anna Perkowska Konrad Fischer Dmitrij Frishman Ruedi Fries Marek Switonski Alexander Kind Dieter Saur Angelika Schnieke Krzysztof Flisikowski Porcine familial adenomatous polyposis model enables systematic analysis of early events in adenoma progression
description	Abstract We compared gene expression in low and high-grade intraepithelial dysplastic polyps from pigs carrying an APC 1311 truncating mutation orthologous to human APC 1309 , analysing whole samples and microdissected dysplastic epithelium. Gene set enrichment analysis revealed differential expression of gene sets similar to human normal mucosa versus T1 stage polyps. Transcriptome analysis of whole samples revealed many differentially-expressed genes reflecting immune infiltration. Analysis of microdissected dysplastic epithelium was markedly different and showed increased expression in high-grade intraepithelial neoplasia of several genes known to be involved in human CRC; and revealed possible new roles for GBP6 and PLXND1. The pig model thus facilitates analysis of CRC pathogenesis.
format	article
author	Tatiana Flisikowska Monika Stachowiak Hongen Xu Alexandra Wagner Alejandra Hernandez-Caceres Christine Wurmser Carolin Perleberg Hubert Pausch Anna Perkowska Konrad Fischer Dmitrij Frishman Ruedi Fries Marek Switonski Alexander Kind Dieter Saur Angelika Schnieke Krzysztof Flisikowski
author_facet	Tatiana Flisikowska Monika Stachowiak Hongen Xu Alexandra Wagner Alejandra Hernandez-Caceres Christine Wurmser Carolin Perleberg Hubert Pausch Anna Perkowska Konrad Fischer Dmitrij Frishman Ruedi Fries Marek Switonski Alexander Kind Dieter Saur Angelika Schnieke Krzysztof Flisikowski
author_sort	Tatiana Flisikowska
title	Porcine familial adenomatous polyposis model enables systematic analysis of early events in adenoma progression
title_short	Porcine familial adenomatous polyposis model enables systematic analysis of early events in adenoma progression
title_full	Porcine familial adenomatous polyposis model enables systematic analysis of early events in adenoma progression
title_fullStr	Porcine familial adenomatous polyposis model enables systematic analysis of early events in adenoma progression
title_full_unstemmed	Porcine familial adenomatous polyposis model enables systematic analysis of early events in adenoma progression
title_sort	porcine familial adenomatous polyposis model enables systematic analysis of early events in adenoma progression
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/72016a41c779481dadcfe7319ad01032
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Porcine familial adenomatous polyposis model enables systematic analysis of early events in adenoma progression

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