BMP2-modified injectable hydrogel for osteogenic differentiation of human periodontal ligament stem cells

Abstract This is the first report on the development of a covalently bone morphogenetic protein-2 (BMP2)-immobilized hydrogel that is suitable for osteogenic differentiation of human periodontal ligament stem cells (hPLSCs). O-propargyl-tyrosine (OpgY) was site-specifically incorporated into BMP2 to...

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Autores principales:	Seung Hun Park, Jin Seon Kwon, Byeong Sung Lee, Ji Hoon Park, Bo Keun Lee, Jeong-Ho Yun, Bun Yeoul Lee, Jae Ho Kim, Byoung Hyun Min, Tae Hyeon Yoo, Moon Suk Kim
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7202879d65c94092bdcc810a5e5f670c
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spelling	oai:doaj.org-article:7202879d65c94092bdcc810a5e5f670c2021-12-02T16:06:56ZBMP2-modified injectable hydrogel for osteogenic differentiation of human periodontal ligament stem cells10.1038/s41598-017-06911-82045-2322https://doaj.org/article/7202879d65c94092bdcc810a5e5f670c2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06911-8https://doaj.org/toc/2045-2322Abstract This is the first report on the development of a covalently bone morphogenetic protein-2 (BMP2)-immobilized hydrogel that is suitable for osteogenic differentiation of human periodontal ligament stem cells (hPLSCs). O-propargyl-tyrosine (OpgY) was site-specifically incorporated into BMP2 to prepare BMP2-OpgY with an alkyne group. The engineered BMP2-OpgY exhibited osteogenic characteristics after in vitro osteogenic differentiation of hPLSCs, indicating the osteogenic ability of BMP2-OpgY. A methoxy polyethylene glycol-(polycaprolactone-(N3)) block copolymer (MC-N3) was prepared as an injectable in situ-forming hydrogel. BMP2 covalently immobilized on an MC hydrogel (MC-BMP2) was prepared quantitatively by a simple biorthogonal reaction between alkyne groups on BMP2-OpgY and azide groups on MC-N3 via a Cu(I)-catalyzed click reaction. The hPLSCs-loaded MC-BMP2 formed a hydrogel almost immediately upon injection into animals. In vivo osteogenic differentiation of hPLSCs in the MC-BMP2 formulation was confirmed by histological staining and gene expression analyses. Histological staining of hPLSC-loaded MC-BMP2 implants showed evidence of mineralized calcium deposits, whereas hPLSC-loaded MC-Cl or BMP2-OpgY mixed with MC-Cl, implants showed no mineral deposits. Additionally, MC-BMP2 induced higher levels of osteogenic gene expression in hPLSCs than in other groups. In conclusion, BMP2-OpgY covalently immobilized on MC-BMP2 induced osteogenic differentiation of hPLSCs as a noninvasive method for bone tissue engineering.Seung Hun ParkJin Seon KwonByeong Sung LeeJi Hoon ParkBo Keun LeeJeong-Ho YunBun Yeoul LeeJae Ho KimByoung Hyun MinTae Hyeon YooMoon Suk KimNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-15 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Seung Hun Park Jin Seon Kwon Byeong Sung Lee Ji Hoon Park Bo Keun Lee Jeong-Ho Yun Bun Yeoul Lee Jae Ho Kim Byoung Hyun Min Tae Hyeon Yoo Moon Suk Kim BMP2-modified injectable hydrogel for osteogenic differentiation of human periodontal ligament stem cells
description	Abstract This is the first report on the development of a covalently bone morphogenetic protein-2 (BMP2)-immobilized hydrogel that is suitable for osteogenic differentiation of human periodontal ligament stem cells (hPLSCs). O-propargyl-tyrosine (OpgY) was site-specifically incorporated into BMP2 to prepare BMP2-OpgY with an alkyne group. The engineered BMP2-OpgY exhibited osteogenic characteristics after in vitro osteogenic differentiation of hPLSCs, indicating the osteogenic ability of BMP2-OpgY. A methoxy polyethylene glycol-(polycaprolactone-(N3)) block copolymer (MC-N3) was prepared as an injectable in situ-forming hydrogel. BMP2 covalently immobilized on an MC hydrogel (MC-BMP2) was prepared quantitatively by a simple biorthogonal reaction between alkyne groups on BMP2-OpgY and azide groups on MC-N3 via a Cu(I)-catalyzed click reaction. The hPLSCs-loaded MC-BMP2 formed a hydrogel almost immediately upon injection into animals. In vivo osteogenic differentiation of hPLSCs in the MC-BMP2 formulation was confirmed by histological staining and gene expression analyses. Histological staining of hPLSC-loaded MC-BMP2 implants showed evidence of mineralized calcium deposits, whereas hPLSC-loaded MC-Cl or BMP2-OpgY mixed with MC-Cl, implants showed no mineral deposits. Additionally, MC-BMP2 induced higher levels of osteogenic gene expression in hPLSCs than in other groups. In conclusion, BMP2-OpgY covalently immobilized on MC-BMP2 induced osteogenic differentiation of hPLSCs as a noninvasive method for bone tissue engineering.
format	article
author	Seung Hun Park Jin Seon Kwon Byeong Sung Lee Ji Hoon Park Bo Keun Lee Jeong-Ho Yun Bun Yeoul Lee Jae Ho Kim Byoung Hyun Min Tae Hyeon Yoo Moon Suk Kim
author_facet	Seung Hun Park Jin Seon Kwon Byeong Sung Lee Ji Hoon Park Bo Keun Lee Jeong-Ho Yun Bun Yeoul Lee Jae Ho Kim Byoung Hyun Min Tae Hyeon Yoo Moon Suk Kim
author_sort	Seung Hun Park
title	BMP2-modified injectable hydrogel for osteogenic differentiation of human periodontal ligament stem cells
title_short	BMP2-modified injectable hydrogel for osteogenic differentiation of human periodontal ligament stem cells
title_full	BMP2-modified injectable hydrogel for osteogenic differentiation of human periodontal ligament stem cells
title_fullStr	BMP2-modified injectable hydrogel for osteogenic differentiation of human periodontal ligament stem cells
title_full_unstemmed	BMP2-modified injectable hydrogel for osteogenic differentiation of human periodontal ligament stem cells
title_sort	bmp2-modified injectable hydrogel for osteogenic differentiation of human periodontal ligament stem cells
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/7202879d65c94092bdcc810a5e5f670c
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BMP2-modified injectable hydrogel for osteogenic differentiation of human periodontal ligament stem cells

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