Zika virus and temperature modulate Elizabethkingia anophelis in Aedes albopictus

Abstract Background Vector-borne pathogens must survive and replicate in the hostile environment of an insect’s midgut before successful dissemination. Midgut microbiota interfere with pathogen infection by activating the basal immunity of the mosquito and by synthesizing pathogen-inhibitory metabol...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Maria G. Onyango, Rachel Lange, Sean Bialosuknia, Anne Payne, Nicholas Mathias, Lili Kuo, Aurelien Vigneron, Dilip Nag, Laura D. Kramer, Alexander T. Ciota
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Zika virus Aedes albopictus Microbiome Elizabethkingia Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/720f45cc46b2476695f187be4d974cde
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:720f45cc46b2476695f187be4d974cde
record_format	dspace
spelling	oai:doaj.org-article:720f45cc46b2476695f187be4d974cde2021-11-14T12:25:21ZZika virus and temperature modulate Elizabethkingia anophelis in Aedes albopictus10.1186/s13071-021-05069-71756-3305https://doaj.org/article/720f45cc46b2476695f187be4d974cde2021-11-01T00:00:00Zhttps://doi.org/10.1186/s13071-021-05069-7https://doaj.org/toc/1756-3305Abstract Background Vector-borne pathogens must survive and replicate in the hostile environment of an insect’s midgut before successful dissemination. Midgut microbiota interfere with pathogen infection by activating the basal immunity of the mosquito and by synthesizing pathogen-inhibitory metabolites. Methods The goal of this study was to assess the influence of Zika virus (ZIKV) infection and increased temperature on Aedes albopictus midgut microbiota. Aedes albopictus were reared at diurnal temperatures of day 28 °C/night 24 °C (L) or day 30 °C/night 26 °C (M). The mosquitoes were given infectious blood meals with 2.0 × 108 PFU/ml ZIKV, and 16S rRNA sequencing was performed on midguts at 7 days post-infectious blood meal exposure. Results Our findings demonstrate that Elizabethkingia anophelis albopictus was associated with Ae. albopictus midguts exposed to ZIKV infectious blood meal. We observed a negative correlation between ZIKV and E. anophelis albopictus in the midguts of Ae. albopictus. Supplemental feeding of Ae. albopictus with E. anophelis aegypti and ZIKV resulted in reduced ZIKV infection rates. Reduced viral loads were detected in Vero cells that were sequentially infected with E. anophelis aegypti and ZIKV, dengue virus (DENV), or chikungunya virus (CHIKV). Conclusions Our findings demonstrate the influence of ZIKV infection and temperature on the Ae. albopictus microbiome along with a negative correlation between ZIKV and E. anophelis albopictus. Our results have important implications for controlling vector-borne pathogens. Graphical AbstractMaria G. OnyangoRachel LangeSean BialosukniaAnne PayneNicholas MathiasLili KuoAurelien VigneronDilip NagLaura D. KramerAlexander T. CiotaBMCarticleZika virusAedes albopictusMicrobiomeElizabethkingiaInfectious and parasitic diseasesRC109-216ENParasites & Vectors, Vol 14, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Zika virus Aedes albopictus Microbiome Elizabethkingia Infectious and parasitic diseases RC109-216
spellingShingle	Zika virus Aedes albopictus Microbiome Elizabethkingia Infectious and parasitic diseases RC109-216 Maria G. Onyango Rachel Lange Sean Bialosuknia Anne Payne Nicholas Mathias Lili Kuo Aurelien Vigneron Dilip Nag Laura D. Kramer Alexander T. Ciota Zika virus and temperature modulate Elizabethkingia anophelis in Aedes albopictus
description	Abstract Background Vector-borne pathogens must survive and replicate in the hostile environment of an insect’s midgut before successful dissemination. Midgut microbiota interfere with pathogen infection by activating the basal immunity of the mosquito and by synthesizing pathogen-inhibitory metabolites. Methods The goal of this study was to assess the influence of Zika virus (ZIKV) infection and increased temperature on Aedes albopictus midgut microbiota. Aedes albopictus were reared at diurnal temperatures of day 28 °C/night 24 °C (L) or day 30 °C/night 26 °C (M). The mosquitoes were given infectious blood meals with 2.0 × 108 PFU/ml ZIKV, and 16S rRNA sequencing was performed on midguts at 7 days post-infectious blood meal exposure. Results Our findings demonstrate that Elizabethkingia anophelis albopictus was associated with Ae. albopictus midguts exposed to ZIKV infectious blood meal. We observed a negative correlation between ZIKV and E. anophelis albopictus in the midguts of Ae. albopictus. Supplemental feeding of Ae. albopictus with E. anophelis aegypti and ZIKV resulted in reduced ZIKV infection rates. Reduced viral loads were detected in Vero cells that were sequentially infected with E. anophelis aegypti and ZIKV, dengue virus (DENV), or chikungunya virus (CHIKV). Conclusions Our findings demonstrate the influence of ZIKV infection and temperature on the Ae. albopictus microbiome along with a negative correlation between ZIKV and E. anophelis albopictus. Our results have important implications for controlling vector-borne pathogens. Graphical Abstract
format	article
author	Maria G. Onyango Rachel Lange Sean Bialosuknia Anne Payne Nicholas Mathias Lili Kuo Aurelien Vigneron Dilip Nag Laura D. Kramer Alexander T. Ciota
author_facet	Maria G. Onyango Rachel Lange Sean Bialosuknia Anne Payne Nicholas Mathias Lili Kuo Aurelien Vigneron Dilip Nag Laura D. Kramer Alexander T. Ciota
author_sort	Maria G. Onyango
title	Zika virus and temperature modulate Elizabethkingia anophelis in Aedes albopictus
title_short	Zika virus and temperature modulate Elizabethkingia anophelis in Aedes albopictus
title_full	Zika virus and temperature modulate Elizabethkingia anophelis in Aedes albopictus
title_fullStr	Zika virus and temperature modulate Elizabethkingia anophelis in Aedes albopictus
title_full_unstemmed	Zika virus and temperature modulate Elizabethkingia anophelis in Aedes albopictus
title_sort	zika virus and temperature modulate elizabethkingia anophelis in aedes albopictus
publisher	BMC
publishDate	2021
url	https://doaj.org/article/720f45cc46b2476695f187be4d974cde
work_keys_str_mv	AT mariagonyango zikavirusandtemperaturemodulateelizabethkingiaanophelisinaedesalbopictus AT rachellange zikavirusandtemperaturemodulateelizabethkingiaanophelisinaedesalbopictus AT seanbialosuknia zikavirusandtemperaturemodulateelizabethkingiaanophelisinaedesalbopictus AT annepayne zikavirusandtemperaturemodulateelizabethkingiaanophelisinaedesalbopictus AT nicholasmathias zikavirusandtemperaturemodulateelizabethkingiaanophelisinaedesalbopictus AT lilikuo zikavirusandtemperaturemodulateelizabethkingiaanophelisinaedesalbopictus AT aurelienvigneron zikavirusandtemperaturemodulateelizabethkingiaanophelisinaedesalbopictus AT dilipnag zikavirusandtemperaturemodulateelizabethkingiaanophelisinaedesalbopictus AT lauradkramer zikavirusandtemperaturemodulateelizabethkingiaanophelisinaedesalbopictus AT alexandertciota zikavirusandtemperaturemodulateelizabethkingiaanophelisinaedesalbopictus
_version_	1718429234286821376

Zika virus and temperature modulate Elizabethkingia anophelis in Aedes albopictus

Ejemplares similares