Generation of panels of anti-idiotypic single-chain variable fragments mimicking Cry2Aa toxin using the chain shuffling technique

Here, we firstly reported to generate panels of anti-idiotypic single-chain variable fragments (scFvs) of Bacillus thuringiensis Cry2Aa toxin by chain shuffling method. Light- and heavy-chain-shuffled libraries based on two parental clones were constructed. The signal of the light-chain-shuffled lib...

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Autores principales:	Yuan Liu, Manman Lin, Jiaxin Wu, Xiaodan Hu, Xiao Zhang, Chongxin Xu, Jianfeng Zhong, Yajing Xie, Cunzheng Zhang, Chuping Luo, Xianjin Liu
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2018
Materias:	chain shuffling anti-idiotypic antibodies single-chain variable fragments (scfvs) cry2aa toxin Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/72146593faf745d2be8d59cc1348edcc
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spelling	oai:doaj.org-article:72146593faf745d2be8d59cc1348edcc2021-11-26T11:19:46ZGeneration of panels of anti-idiotypic single-chain variable fragments mimicking Cry2Aa toxin using the chain shuffling technique0954-01051465-344310.1080/09540105.2018.1440535https://doaj.org/article/72146593faf745d2be8d59cc1348edcc2018-01-01T00:00:00Zhttp://dx.doi.org/10.1080/09540105.2018.1440535https://doaj.org/toc/0954-0105https://doaj.org/toc/1465-3443Here, we firstly reported to generate panels of anti-idiotypic single-chain variable fragments (scFvs) of Bacillus thuringiensis Cry2Aa toxin by chain shuffling method. Light- and heavy-chain-shuffled libraries based on two parental clones were constructed. The signal of the light-chain-shuffled library was 4.2-fold higher than the heavy one. Solely after one round panning, eight mutants were rapidly isolated from the chain-shuffled libraries. The apparent affinity of the best clones was 2.65 × 106 M−1, a 2.59-fold improvement over the parental clone. The binding abilities between anti-idiotypic scFvs with brush border membrane vesicle of Plutella xylostella larvae were also determined. This work demonstrated that chain shuffling could rapidly generate panels of antibodies with similar binding abilities but of the distinct idiotypic composition. The serials of mutants can be used to map the binding epitope of the receptors in target insects instead of Cry2Aa toxin.Yuan LiuManman LinJiaxin WuXiaodan HuXiao ZhangChongxin XuJianfeng ZhongYajing XieCunzheng ZhangChuping LuoXianjin LiuTaylor & Francis Grouparticlechain shufflinganti-idiotypic antibodiessingle-chain variable fragments (scfvs)cry2aa toxinAgriculture (General)S1-972Immunologic diseases. AllergyRC581-607ENFood and Agricultural Immunology, Vol 29, Iss 1, Pp 735-743 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	chain shuffling anti-idiotypic antibodies single-chain variable fragments (scfvs) cry2aa toxin Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607
spellingShingle	chain shuffling anti-idiotypic antibodies single-chain variable fragments (scfvs) cry2aa toxin Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607 Yuan Liu Manman Lin Jiaxin Wu Xiaodan Hu Xiao Zhang Chongxin Xu Jianfeng Zhong Yajing Xie Cunzheng Zhang Chuping Luo Xianjin Liu Generation of panels of anti-idiotypic single-chain variable fragments mimicking Cry2Aa toxin using the chain shuffling technique
description	Here, we firstly reported to generate panels of anti-idiotypic single-chain variable fragments (scFvs) of Bacillus thuringiensis Cry2Aa toxin by chain shuffling method. Light- and heavy-chain-shuffled libraries based on two parental clones were constructed. The signal of the light-chain-shuffled library was 4.2-fold higher than the heavy one. Solely after one round panning, eight mutants were rapidly isolated from the chain-shuffled libraries. The apparent affinity of the best clones was 2.65 × 106 M−1, a 2.59-fold improvement over the parental clone. The binding abilities between anti-idiotypic scFvs with brush border membrane vesicle of Plutella xylostella larvae were also determined. This work demonstrated that chain shuffling could rapidly generate panels of antibodies with similar binding abilities but of the distinct idiotypic composition. The serials of mutants can be used to map the binding epitope of the receptors in target insects instead of Cry2Aa toxin.
format	article
author	Yuan Liu Manman Lin Jiaxin Wu Xiaodan Hu Xiao Zhang Chongxin Xu Jianfeng Zhong Yajing Xie Cunzheng Zhang Chuping Luo Xianjin Liu
author_facet	Yuan Liu Manman Lin Jiaxin Wu Xiaodan Hu Xiao Zhang Chongxin Xu Jianfeng Zhong Yajing Xie Cunzheng Zhang Chuping Luo Xianjin Liu
author_sort	Yuan Liu
title	Generation of panels of anti-idiotypic single-chain variable fragments mimicking Cry2Aa toxin using the chain shuffling technique
title_short	Generation of panels of anti-idiotypic single-chain variable fragments mimicking Cry2Aa toxin using the chain shuffling technique
title_full	Generation of panels of anti-idiotypic single-chain variable fragments mimicking Cry2Aa toxin using the chain shuffling technique
title_fullStr	Generation of panels of anti-idiotypic single-chain variable fragments mimicking Cry2Aa toxin using the chain shuffling technique
title_full_unstemmed	Generation of panels of anti-idiotypic single-chain variable fragments mimicking Cry2Aa toxin using the chain shuffling technique
title_sort	generation of panels of anti-idiotypic single-chain variable fragments mimicking cry2aa toxin using the chain shuffling technique
publisher	Taylor & Francis Group
publishDate	2018
url	https://doaj.org/article/72146593faf745d2be8d59cc1348edcc
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Generation of panels of anti-idiotypic single-chain variable fragments mimicking Cry2Aa toxin using the chain shuffling technique

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