Sensitive detection of SARS-CoV-2 seroconversion by flow cytometry reveals the presence of nucleoprotein-reactive antibodies in unexposed individuals

Egia-Mendikute et al. develop a multiplexed flow cytometric bead array to simultaneously detect antibodies reactive to three immunogenic SARS-CoV-2 proteins. More sensitive than ELISA, they detected N-reactive antibodies in COVID-19-negative individuals with this assay, showing it to have superior p...

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Autores principales:	Leire Egia-Mendikute, Alexandre Bosch, Endika Prieto-Fernández, So Young Lee, Borja Jiménez-Lasheras, Ana García del Río, Asier Antoñana-Vildosola, Chiara Bruzzone, Maider Bizkarguenaga, Nieves Embade, Rubén Gil-Redondo, María Luz Martínez-Chantar, Marcos López-Hoyos, Nicola G. A. Abrescia, José M. Mato, Óscar Millet, Asís Palazón
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/721616baa10e46b992979a7d09ffe6af
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spelling	oai:doaj.org-article:721616baa10e46b992979a7d09ffe6af2021-12-02T17:32:54ZSensitive detection of SARS-CoV-2 seroconversion by flow cytometry reveals the presence of nucleoprotein-reactive antibodies in unexposed individuals10.1038/s42003-021-02011-62399-3642https://doaj.org/article/721616baa10e46b992979a7d09ffe6af2021-04-01T00:00:00Zhttps://doi.org/10.1038/s42003-021-02011-6https://doaj.org/toc/2399-3642Egia-Mendikute et al. develop a multiplexed flow cytometric bead array to simultaneously detect antibodies reactive to three immunogenic SARS-CoV-2 proteins. More sensitive than ELISA, they detected N-reactive antibodies in COVID-19-negative individuals with this assay, showing it to have superior potential to detect low antibody titres compared to current gold standard serology methods.Leire Egia-MendikuteAlexandre BoschEndika Prieto-FernándezSo Young LeeBorja Jiménez-LasherasAna García del RíoAsier Antoñana-VildosolaChiara BruzzoneMaider BizkarguenagaNieves EmbadeRubén Gil-RedondoMaría Luz Martínez-ChantarMarcos López-HoyosNicola G. A. AbresciaJosé M. MatoÓscar MilletAsís PalazónNature PortfolioarticleBiology (General)QH301-705.5ENCommunications Biology, Vol 4, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Leire Egia-Mendikute Alexandre Bosch Endika Prieto-Fernández So Young Lee Borja Jiménez-Lasheras Ana García del Río Asier Antoñana-Vildosola Chiara Bruzzone Maider Bizkarguenaga Nieves Embade Rubén Gil-Redondo María Luz Martínez-Chantar Marcos López-Hoyos Nicola G. A. Abrescia José M. Mato Óscar Millet Asís Palazón Sensitive detection of SARS-CoV-2 seroconversion by flow cytometry reveals the presence of nucleoprotein-reactive antibodies in unexposed individuals
description	Egia-Mendikute et al. develop a multiplexed flow cytometric bead array to simultaneously detect antibodies reactive to three immunogenic SARS-CoV-2 proteins. More sensitive than ELISA, they detected N-reactive antibodies in COVID-19-negative individuals with this assay, showing it to have superior potential to detect low antibody titres compared to current gold standard serology methods.
format	article
author	Leire Egia-Mendikute Alexandre Bosch Endika Prieto-Fernández So Young Lee Borja Jiménez-Lasheras Ana García del Río Asier Antoñana-Vildosola Chiara Bruzzone Maider Bizkarguenaga Nieves Embade Rubén Gil-Redondo María Luz Martínez-Chantar Marcos López-Hoyos Nicola G. A. Abrescia José M. Mato Óscar Millet Asís Palazón
author_facet	Leire Egia-Mendikute Alexandre Bosch Endika Prieto-Fernández So Young Lee Borja Jiménez-Lasheras Ana García del Río Asier Antoñana-Vildosola Chiara Bruzzone Maider Bizkarguenaga Nieves Embade Rubén Gil-Redondo María Luz Martínez-Chantar Marcos López-Hoyos Nicola G. A. Abrescia José M. Mato Óscar Millet Asís Palazón
author_sort	Leire Egia-Mendikute
title	Sensitive detection of SARS-CoV-2 seroconversion by flow cytometry reveals the presence of nucleoprotein-reactive antibodies in unexposed individuals
title_short	Sensitive detection of SARS-CoV-2 seroconversion by flow cytometry reveals the presence of nucleoprotein-reactive antibodies in unexposed individuals
title_full	Sensitive detection of SARS-CoV-2 seroconversion by flow cytometry reveals the presence of nucleoprotein-reactive antibodies in unexposed individuals
title_fullStr	Sensitive detection of SARS-CoV-2 seroconversion by flow cytometry reveals the presence of nucleoprotein-reactive antibodies in unexposed individuals
title_full_unstemmed	Sensitive detection of SARS-CoV-2 seroconversion by flow cytometry reveals the presence of nucleoprotein-reactive antibodies in unexposed individuals
title_sort	sensitive detection of sars-cov-2 seroconversion by flow cytometry reveals the presence of nucleoprotein-reactive antibodies in unexposed individuals
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/721616baa10e46b992979a7d09ffe6af
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Sensitive detection of SARS-CoV-2 seroconversion by flow cytometry reveals the presence of nucleoprotein-reactive antibodies in unexposed individuals

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