Human umbilical cord blood-derived mesenchymal stem cells promote vascular growth in vivo.

Stem cell therapies are promising strategies to regenerate human injured tissues, including ischemic myocardium. Here, we examined the acquisition of properties associated with vascular growth by human umbilical cord blood-derived mesenchymal stem cells (UCBMSCs), and whether they promoted vascular...

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Autores principales:	Santiago Roura, Juli R Bagó, Carolina Soler-Botija, Josep M Pujal, Carolina Gálvez-Montón, Cristina Prat-Vidal, Aida Llucià-Valldeperas, Jerónimo Blanco, Antoni Bayes-Genis
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7216f670f728488a8073734c4bb14404
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spelling	oai:doaj.org-article:7216f670f728488a8073734c4bb144042021-11-18T08:08:29ZHuman umbilical cord blood-derived mesenchymal stem cells promote vascular growth in vivo.1932-620310.1371/journal.pone.0049447https://doaj.org/article/7216f670f728488a8073734c4bb144042012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23166670/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Stem cell therapies are promising strategies to regenerate human injured tissues, including ischemic myocardium. Here, we examined the acquisition of properties associated with vascular growth by human umbilical cord blood-derived mesenchymal stem cells (UCBMSCs), and whether they promoted vascular growth in vivo. UCBMSCs were induced in endothelial cell-specific growth medium (EGM-2) acquiring new cell markers, increased Ac-LDL uptake, and migratory capacity as assessed by qRT-PCR, Western blotting, indirect immunofluorescence, and invasion assays. Angiogenic and vasculogenic potentials could be anticipated by in vitro experiments showing self organization into Matrigel-mediated cell networks, and activation of circulating angiogenic-supportive myeloid cells. In mice, following subcutaneous co-injection with Matrigel, UCBMSCs modified to co-express bioluminescent (luciferases) and fluorescent proteins were demonstrated to participate in the formation of new microvasculature connected with the host circulatory system. Response of UCBMSCs to ischemia was explored in a mouse model of acute myocardial infarction (MI). UCBMSCs transplanted using a fibrin patch survived 4 weeks post-implantation and organized into CD31(+)network structures above the infarcted myocardium. MI-treated animals showed a reduced infarct scar and a larger vessel-occupied area in comparison with MI-control animals. Taken together, the presented results show that UCBMSCs can be induced in vitro to acquire angiogenic and vasculogenic properties and contribute to vascular growth in vivo.Santiago RouraJuli R BagóCarolina Soler-BotijaJosep M PujalCarolina Gálvez-MontónCristina Prat-VidalAida Llucià-ValldeperasJerónimo BlancoAntoni Bayes-GenisPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 11, p e49447 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Santiago Roura Juli R Bagó Carolina Soler-Botija Josep M Pujal Carolina Gálvez-Montón Cristina Prat-Vidal Aida Llucià-Valldeperas Jerónimo Blanco Antoni Bayes-Genis Human umbilical cord blood-derived mesenchymal stem cells promote vascular growth in vivo.
description	Stem cell therapies are promising strategies to regenerate human injured tissues, including ischemic myocardium. Here, we examined the acquisition of properties associated with vascular growth by human umbilical cord blood-derived mesenchymal stem cells (UCBMSCs), and whether they promoted vascular growth in vivo. UCBMSCs were induced in endothelial cell-specific growth medium (EGM-2) acquiring new cell markers, increased Ac-LDL uptake, and migratory capacity as assessed by qRT-PCR, Western blotting, indirect immunofluorescence, and invasion assays. Angiogenic and vasculogenic potentials could be anticipated by in vitro experiments showing self organization into Matrigel-mediated cell networks, and activation of circulating angiogenic-supportive myeloid cells. In mice, following subcutaneous co-injection with Matrigel, UCBMSCs modified to co-express bioluminescent (luciferases) and fluorescent proteins were demonstrated to participate in the formation of new microvasculature connected with the host circulatory system. Response of UCBMSCs to ischemia was explored in a mouse model of acute myocardial infarction (MI). UCBMSCs transplanted using a fibrin patch survived 4 weeks post-implantation and organized into CD31(+)network structures above the infarcted myocardium. MI-treated animals showed a reduced infarct scar and a larger vessel-occupied area in comparison with MI-control animals. Taken together, the presented results show that UCBMSCs can be induced in vitro to acquire angiogenic and vasculogenic properties and contribute to vascular growth in vivo.
format	article
author	Santiago Roura Juli R Bagó Carolina Soler-Botija Josep M Pujal Carolina Gálvez-Montón Cristina Prat-Vidal Aida Llucià-Valldeperas Jerónimo Blanco Antoni Bayes-Genis
author_facet	Santiago Roura Juli R Bagó Carolina Soler-Botija Josep M Pujal Carolina Gálvez-Montón Cristina Prat-Vidal Aida Llucià-Valldeperas Jerónimo Blanco Antoni Bayes-Genis
author_sort	Santiago Roura
title	Human umbilical cord blood-derived mesenchymal stem cells promote vascular growth in vivo.
title_short	Human umbilical cord blood-derived mesenchymal stem cells promote vascular growth in vivo.
title_full	Human umbilical cord blood-derived mesenchymal stem cells promote vascular growth in vivo.
title_fullStr	Human umbilical cord blood-derived mesenchymal stem cells promote vascular growth in vivo.
title_full_unstemmed	Human umbilical cord blood-derived mesenchymal stem cells promote vascular growth in vivo.
title_sort	human umbilical cord blood-derived mesenchymal stem cells promote vascular growth in vivo.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/7216f670f728488a8073734c4bb14404
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Human umbilical cord blood-derived mesenchymal stem cells promote vascular growth in vivo.

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