Anti-metastatic effect of methylprednisolone targeting vascular endothelial cells under surgical stress

Abstract Perioperative systemic inflammation induced by surgical stress elevates the risk of hematogenous cancer metastasis. This study investigated the anti-metastatic effects and mechanisms of methylprednisolone (MP) administration for surgical stress. We examined the effects of MP on the expressi...

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Autores principales:	Takaomi Hagi, Yukinori Kurokawa, Noboru Kobayashi, Tsuyoshi Takahashi, Takuro Saito, Kotaro Yamashita, Koji Tanaka, Tomoki Makino, Makoto Yamasaki, Kiyokazu Nakajima, Hidetoshi Eguchi, Yuichiro Doki
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/721c71d72d4545deaa88e9e452b89840
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spelling	oai:doaj.org-article:721c71d72d4545deaa88e9e452b898402021-12-02T16:30:24ZAnti-metastatic effect of methylprednisolone targeting vascular endothelial cells under surgical stress10.1038/s41598-021-85241-22045-2322https://doaj.org/article/721c71d72d4545deaa88e9e452b898402021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-85241-2https://doaj.org/toc/2045-2322Abstract Perioperative systemic inflammation induced by surgical stress elevates the risk of hematogenous cancer metastasis. This study investigated the anti-metastatic effects and mechanisms of methylprednisolone (MP) administration for surgical stress. We examined the effects of MP on the expression of adhesion molecules in human vascular endothelial cells and in a murine hepatic metastasis model under lipopolysaccharide (LPS) administration, which mimics systemic inflammation induced by surgical stress. Serum E-selectin level was measured in blood samples obtained from 32 gastric cancer patients who were randomly assigned to treat preoperatively with or without MP. The expression of E-selectin in LPS-induced vascular endothelial cells was suppressed by MP. An adhesion assay showed the number of LPS-induced adherent tumour cells was significantly lower following MP. In the in vivo study, LPS significantly elevated the number of hepatic metastases, but pretreatment with MP before LPS significantly inhibited this elevation. The LPS-induced expression of E-selectin in the vascular endothelium of the portal vein was suppressed by MP. In human clinical samples, serum E-selectin level was significantly decreased by preoperative MP. Suppression of surgically induced systemic inflammation by MP administration might prevent hematogenous cancer metastases by suppressing the induction of E-selectin expression in the vascular endothelium.Takaomi HagiYukinori KurokawaNoboru KobayashiTsuyoshi TakahashiTakuro SaitoKotaro YamashitaKoji TanakaTomoki MakinoMakoto YamasakiKiyokazu NakajimaHidetoshi EguchiYuichiro DokiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Takaomi Hagi Yukinori Kurokawa Noboru Kobayashi Tsuyoshi Takahashi Takuro Saito Kotaro Yamashita Koji Tanaka Tomoki Makino Makoto Yamasaki Kiyokazu Nakajima Hidetoshi Eguchi Yuichiro Doki Anti-metastatic effect of methylprednisolone targeting vascular endothelial cells under surgical stress
description	Abstract Perioperative systemic inflammation induced by surgical stress elevates the risk of hematogenous cancer metastasis. This study investigated the anti-metastatic effects and mechanisms of methylprednisolone (MP) administration for surgical stress. We examined the effects of MP on the expression of adhesion molecules in human vascular endothelial cells and in a murine hepatic metastasis model under lipopolysaccharide (LPS) administration, which mimics systemic inflammation induced by surgical stress. Serum E-selectin level was measured in blood samples obtained from 32 gastric cancer patients who were randomly assigned to treat preoperatively with or without MP. The expression of E-selectin in LPS-induced vascular endothelial cells was suppressed by MP. An adhesion assay showed the number of LPS-induced adherent tumour cells was significantly lower following MP. In the in vivo study, LPS significantly elevated the number of hepatic metastases, but pretreatment with MP before LPS significantly inhibited this elevation. The LPS-induced expression of E-selectin in the vascular endothelium of the portal vein was suppressed by MP. In human clinical samples, serum E-selectin level was significantly decreased by preoperative MP. Suppression of surgically induced systemic inflammation by MP administration might prevent hematogenous cancer metastases by suppressing the induction of E-selectin expression in the vascular endothelium.
format	article
author	Takaomi Hagi Yukinori Kurokawa Noboru Kobayashi Tsuyoshi Takahashi Takuro Saito Kotaro Yamashita Koji Tanaka Tomoki Makino Makoto Yamasaki Kiyokazu Nakajima Hidetoshi Eguchi Yuichiro Doki
author_facet	Takaomi Hagi Yukinori Kurokawa Noboru Kobayashi Tsuyoshi Takahashi Takuro Saito Kotaro Yamashita Koji Tanaka Tomoki Makino Makoto Yamasaki Kiyokazu Nakajima Hidetoshi Eguchi Yuichiro Doki
author_sort	Takaomi Hagi
title	Anti-metastatic effect of methylprednisolone targeting vascular endothelial cells under surgical stress
title_short	Anti-metastatic effect of methylprednisolone targeting vascular endothelial cells under surgical stress
title_full	Anti-metastatic effect of methylprednisolone targeting vascular endothelial cells under surgical stress
title_fullStr	Anti-metastatic effect of methylprednisolone targeting vascular endothelial cells under surgical stress
title_full_unstemmed	Anti-metastatic effect of methylprednisolone targeting vascular endothelial cells under surgical stress
title_sort	anti-metastatic effect of methylprednisolone targeting vascular endothelial cells under surgical stress
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/721c71d72d4545deaa88e9e452b89840
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Anti-metastatic effect of methylprednisolone targeting vascular endothelial cells under surgical stress

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