Detection of cancer stem cells by EMT-specific biomarker-based peptide ligands

Abstract The occurrence of epithelial-mesenchymal transition (EMT) within tumors, which enables invasion and metastasis, is linked to cancer stem cells (CSCs) with drug and radiation resistance. We used two specific peptides, F7 and SP peptides, to detect EMT derived cells or CSCs. Human tongue squa...

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Autores principales:	Yi-An Chen, Cheau-Ling Ho, Min-Tzu Ku, Luen Hwu, Cheng-Hsiu Lu, Sain-Jhih Chiu, Wen-Yi Chang, Ren-Shyan Liu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7223393fcab74b7685835304f7d72b7c
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spelling	oai:doaj.org-article:7223393fcab74b7685835304f7d72b7c2021-11-21T12:25:19ZDetection of cancer stem cells by EMT-specific biomarker-based peptide ligands10.1038/s41598-021-01138-02045-2322https://doaj.org/article/7223393fcab74b7685835304f7d72b7c2021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-01138-0https://doaj.org/toc/2045-2322Abstract The occurrence of epithelial-mesenchymal transition (EMT) within tumors, which enables invasion and metastasis, is linked to cancer stem cells (CSCs) with drug and radiation resistance. We used two specific peptides, F7 and SP peptides, to detect EMT derived cells or CSCs. Human tongue squamous carcinoma cell line-SAS transfected with reporter genes was generated and followed by spheroid culture. A small molecule inhibitor-Unc0642 and low-dose ionizing radiation (IR) were used for induction of EMT. Confocal microscopic imaging and fluorescence-activated cell sorting analysis were performed to evaluate the binding ability and specificity of peptides. A SAS xenograft mouse model with EMT induction was established for assessing the binding affinity of peptides. The results showed that F7 and SP peptides not only specifically penetrated into cytoplasm of SAS cells but also bound to EMT derived cells and CSCs with high nucleolin and vimentin expression. In addition, the expression of CSC marker and the binding of peptides were increased in tumors isolated from Unc0642/IR-treated groups. Our study demonstrates the potential of these peptides for detecting EMT derived cells or CSCs and might provide an alternative isolation method for these subpopulations within the tumor in the future.Yi-An ChenCheau-Ling HoMin-Tzu KuLuen HwuCheng-Hsiu LuSain-Jhih ChiuWen-Yi ChangRen-Shyan LiuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yi-An Chen Cheau-Ling Ho Min-Tzu Ku Luen Hwu Cheng-Hsiu Lu Sain-Jhih Chiu Wen-Yi Chang Ren-Shyan Liu Detection of cancer stem cells by EMT-specific biomarker-based peptide ligands
description	Abstract The occurrence of epithelial-mesenchymal transition (EMT) within tumors, which enables invasion and metastasis, is linked to cancer stem cells (CSCs) with drug and radiation resistance. We used two specific peptides, F7 and SP peptides, to detect EMT derived cells or CSCs. Human tongue squamous carcinoma cell line-SAS transfected with reporter genes was generated and followed by spheroid culture. A small molecule inhibitor-Unc0642 and low-dose ionizing radiation (IR) were used for induction of EMT. Confocal microscopic imaging and fluorescence-activated cell sorting analysis were performed to evaluate the binding ability and specificity of peptides. A SAS xenograft mouse model with EMT induction was established for assessing the binding affinity of peptides. The results showed that F7 and SP peptides not only specifically penetrated into cytoplasm of SAS cells but also bound to EMT derived cells and CSCs with high nucleolin and vimentin expression. In addition, the expression of CSC marker and the binding of peptides were increased in tumors isolated from Unc0642/IR-treated groups. Our study demonstrates the potential of these peptides for detecting EMT derived cells or CSCs and might provide an alternative isolation method for these subpopulations within the tumor in the future.
format	article
author	Yi-An Chen Cheau-Ling Ho Min-Tzu Ku Luen Hwu Cheng-Hsiu Lu Sain-Jhih Chiu Wen-Yi Chang Ren-Shyan Liu
author_facet	Yi-An Chen Cheau-Ling Ho Min-Tzu Ku Luen Hwu Cheng-Hsiu Lu Sain-Jhih Chiu Wen-Yi Chang Ren-Shyan Liu
author_sort	Yi-An Chen
title	Detection of cancer stem cells by EMT-specific biomarker-based peptide ligands
title_short	Detection of cancer stem cells by EMT-specific biomarker-based peptide ligands
title_full	Detection of cancer stem cells by EMT-specific biomarker-based peptide ligands
title_fullStr	Detection of cancer stem cells by EMT-specific biomarker-based peptide ligands
title_full_unstemmed	Detection of cancer stem cells by EMT-specific biomarker-based peptide ligands
title_sort	detection of cancer stem cells by emt-specific biomarker-based peptide ligands
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/7223393fcab74b7685835304f7d72b7c
work_keys_str_mv	AT yianchen detectionofcancerstemcellsbyemtspecificbiomarkerbasedpeptideligands AT cheaulingho detectionofcancerstemcellsbyemtspecificbiomarkerbasedpeptideligands AT mintzuku detectionofcancerstemcellsbyemtspecificbiomarkerbasedpeptideligands AT luenhwu detectionofcancerstemcellsbyemtspecificbiomarkerbasedpeptideligands AT chenghsiulu detectionofcancerstemcellsbyemtspecificbiomarkerbasedpeptideligands AT sainjhihchiu detectionofcancerstemcellsbyemtspecificbiomarkerbasedpeptideligands AT wenyichang detectionofcancerstemcellsbyemtspecificbiomarkerbasedpeptideligands AT renshyanliu detectionofcancerstemcellsbyemtspecificbiomarkerbasedpeptideligands
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Detection of cancer stem cells by EMT-specific biomarker-based peptide ligands

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