Human Paramyxovirus Infections Induce T Cells That Cross-React with Zoonotic Henipaviruses

ABSTRACT Humans are infected with paramyxoviruses of different genera early in life, which induce cytotoxic T cells that may recognize conserved epitopes. This raises the question of whether cross-reactive T cells induced by antecedent paramyxovirus infections provide partial protection against high...

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Autores principales:	Rory D. de Vries, Alwin de Jong, R. Joyce Verburgh, Lucie Sauerhering, Gijsbert P. van Nierop, Robert S. van Binnendijk, Albert D. M. E. Osterhaus, Andrea Maisner, Marion P. G. Koopmans, Rik L. de Swart
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2020
Materias:	paramyxovirus T cells measles virus Nipah virus human parainfluenza virus Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/722e6a193a4149919c213b82b3955a5d
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spelling	oai:doaj.org-article:722e6a193a4149919c213b82b3955a5d2021-11-15T15:56:44ZHuman Paramyxovirus Infections Induce T Cells That Cross-React with Zoonotic Henipaviruses10.1128/mBio.00972-202150-7511https://doaj.org/article/722e6a193a4149919c213b82b3955a5d2020-08-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.00972-20https://doaj.org/toc/2150-7511ABSTRACT Humans are infected with paramyxoviruses of different genera early in life, which induce cytotoxic T cells that may recognize conserved epitopes. This raises the question of whether cross-reactive T cells induced by antecedent paramyxovirus infections provide partial protection against highly lethal zoonotic Nipah virus infections. By characterizing a measles virus-specific but paramyxovirus cross-reactive human T cell clone, we discovered a highly conserved HLA-B*1501-restricted T cell epitope in the fusion protein. Using peptides, tetramers, and single cell sorting, we isolated a parainfluenza virus-specific T cell clone from a healthy adult and showed that both clones cleared Nipah virus-infected cells. We identified multiple conserved hot spots in paramyxovirus proteomes that contain other potentially cross-reactive epitopes. Our data suggest that, depending on HLA haplotype and history of paramyxovirus exposures, humans may have cross-reactive T cells that provide protection against Nipah virus. The effect of preferential boosting of these cross-reactive epitopes needs to be further studied in light of paramyxovirus vaccination studies. IMPORTANCE Humans encounter multiple paramyxoviruses early in life. This study shows that infection with common paramyxoviruses can induce T cells cross-reactive with the highly pathogenic Nipah virus. This demonstrates that the combination of paramyxovirus infection history and HLA haplotype affects immunity to phylogenetically related zoonotic paramyxoviruses.Rory D. de VriesAlwin de JongR. Joyce VerburghLucie SauerheringGijsbert P. van NieropRobert S. van BinnendijkAlbert D. M. E. OsterhausAndrea MaisnerMarion P. G. KoopmansRik L. de SwartAmerican Society for MicrobiologyarticleparamyxovirusT cellsmeasles virusNipah virushuman parainfluenza virusMicrobiologyQR1-502ENmBio, Vol 11, Iss 4 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	paramyxovirus T cells measles virus Nipah virus human parainfluenza virus Microbiology QR1-502
spellingShingle	paramyxovirus T cells measles virus Nipah virus human parainfluenza virus Microbiology QR1-502 Rory D. de Vries Alwin de Jong R. Joyce Verburgh Lucie Sauerhering Gijsbert P. van Nierop Robert S. van Binnendijk Albert D. M. E. Osterhaus Andrea Maisner Marion P. G. Koopmans Rik L. de Swart Human Paramyxovirus Infections Induce T Cells That Cross-React with Zoonotic Henipaviruses
description	ABSTRACT Humans are infected with paramyxoviruses of different genera early in life, which induce cytotoxic T cells that may recognize conserved epitopes. This raises the question of whether cross-reactive T cells induced by antecedent paramyxovirus infections provide partial protection against highly lethal zoonotic Nipah virus infections. By characterizing a measles virus-specific but paramyxovirus cross-reactive human T cell clone, we discovered a highly conserved HLA-B*1501-restricted T cell epitope in the fusion protein. Using peptides, tetramers, and single cell sorting, we isolated a parainfluenza virus-specific T cell clone from a healthy adult and showed that both clones cleared Nipah virus-infected cells. We identified multiple conserved hot spots in paramyxovirus proteomes that contain other potentially cross-reactive epitopes. Our data suggest that, depending on HLA haplotype and history of paramyxovirus exposures, humans may have cross-reactive T cells that provide protection against Nipah virus. The effect of preferential boosting of these cross-reactive epitopes needs to be further studied in light of paramyxovirus vaccination studies. IMPORTANCE Humans encounter multiple paramyxoviruses early in life. This study shows that infection with common paramyxoviruses can induce T cells cross-reactive with the highly pathogenic Nipah virus. This demonstrates that the combination of paramyxovirus infection history and HLA haplotype affects immunity to phylogenetically related zoonotic paramyxoviruses.
format	article
author	Rory D. de Vries Alwin de Jong R. Joyce Verburgh Lucie Sauerhering Gijsbert P. van Nierop Robert S. van Binnendijk Albert D. M. E. Osterhaus Andrea Maisner Marion P. G. Koopmans Rik L. de Swart
author_facet	Rory D. de Vries Alwin de Jong R. Joyce Verburgh Lucie Sauerhering Gijsbert P. van Nierop Robert S. van Binnendijk Albert D. M. E. Osterhaus Andrea Maisner Marion P. G. Koopmans Rik L. de Swart
author_sort	Rory D. de Vries
title	Human Paramyxovirus Infections Induce T Cells That Cross-React with Zoonotic Henipaviruses
title_short	Human Paramyxovirus Infections Induce T Cells That Cross-React with Zoonotic Henipaviruses
title_full	Human Paramyxovirus Infections Induce T Cells That Cross-React with Zoonotic Henipaviruses
title_fullStr	Human Paramyxovirus Infections Induce T Cells That Cross-React with Zoonotic Henipaviruses
title_full_unstemmed	Human Paramyxovirus Infections Induce T Cells That Cross-React with Zoonotic Henipaviruses
title_sort	human paramyxovirus infections induce t cells that cross-react with zoonotic henipaviruses
publisher	American Society for Microbiology
publishDate	2020
url	https://doaj.org/article/722e6a193a4149919c213b82b3955a5d
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Human Paramyxovirus Infections Induce T Cells That Cross-React with Zoonotic Henipaviruses

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