Mutation analysis of 2009 pandemic influenza A(H1N1) viruses collected in Japan during the peak phase of the pandemic.
<h4>Background</h4>Pandemic influenza A(H1N1) virus infection quickly circulated worldwide in 2009. In Japan, the first case was reported in May 2009, one month after its outbreak in Mexico. Thereafter, A(H1N1) infection spread widely throughout the country. It is of great importance to...
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oai:doaj.org-article:723046f5766844e4a661c2cc584459482021-11-18T06:54:35ZMutation analysis of 2009 pandemic influenza A(H1N1) viruses collected in Japan during the peak phase of the pandemic.1932-620310.1371/journal.pone.0018956https://doaj.org/article/723046f5766844e4a661c2cc584459482011-04-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21572517/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Pandemic influenza A(H1N1) virus infection quickly circulated worldwide in 2009. In Japan, the first case was reported in May 2009, one month after its outbreak in Mexico. Thereafter, A(H1N1) infection spread widely throughout the country. It is of great importance to profile and understand the situation regarding viral mutations and their circulation in Japan to accumulate a knowledge base and to prepare clinical response platforms before a second pandemic (pdm) wave emerges.<h4>Methodology</h4>A total of 253 swab samples were collected from patients with influenza-like illness in the Osaka, Tokyo, and Chiba areas both in May 2009 and between October 2009 and January 2010. We analyzed partial sequences of the hemagglutinin (HA) and neuraminidase (NA) genes of the 2009 pdm influenza virus in the collected clinical samples. By phylogenetic analysis, we identified major variants of the 2009 pdm influenza virus and critical mutations associated with severe cases, including drug-resistance mutations.<h4>Results and conclusions</h4>Our sequence analysis has revealed that both HA-S220T and NA-N248D are major non-synonymous mutations that clearly discriminate the 2009 pdm influenza viruses identified in the very early phase (May 2009) from those found in the peak phase (October 2009 to January 2010) in Japan. By phylogenetic analysis, we found 14 micro-clades within the viruses collected during the peak phase. Among them, 12 were new micro-clades, while two were previously reported. Oseltamivir resistance-related mutations, i.e., NA-H275Y and NA-N295S, were also detected in sporadic cases in Osaka and Tokyo.Jean-Étienne MorlighemShintaro AokiMami KishimaMitsue HanamiChihiro OgawaAmadu JallohYukari TakahashiYuki KawaiSatomi SagaEiji HayashiToshiaki BanShinyu IzumiAkira WadaMasayuki ManoMegumu FukunagaYoshiyuki KijimaMasashi ShiomiKaoru InoueTakeshi HataYukihiro KoretsuneKoichiro KudoYuji HimenoAizan HiraiKazuo TakahashiYuko Sakai-TagawaKiyoko Iwatsuki-HorimotoYoshihiro KawaokaYoshihide HayashizakiToshihisa IshikawaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 4, p e18956 (2011) |
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Medicine R Science Q Jean-Étienne Morlighem Shintaro Aoki Mami Kishima Mitsue Hanami Chihiro Ogawa Amadu Jalloh Yukari Takahashi Yuki Kawai Satomi Saga Eiji Hayashi Toshiaki Ban Shinyu Izumi Akira Wada Masayuki Mano Megumu Fukunaga Yoshiyuki Kijima Masashi Shiomi Kaoru Inoue Takeshi Hata Yukihiro Koretsune Koichiro Kudo Yuji Himeno Aizan Hirai Kazuo Takahashi Yuko Sakai-Tagawa Kiyoko Iwatsuki-Horimoto Yoshihiro Kawaoka Yoshihide Hayashizaki Toshihisa Ishikawa Mutation analysis of 2009 pandemic influenza A(H1N1) viruses collected in Japan during the peak phase of the pandemic. |
| description |
<h4>Background</h4>Pandemic influenza A(H1N1) virus infection quickly circulated worldwide in 2009. In Japan, the first case was reported in May 2009, one month after its outbreak in Mexico. Thereafter, A(H1N1) infection spread widely throughout the country. It is of great importance to profile and understand the situation regarding viral mutations and their circulation in Japan to accumulate a knowledge base and to prepare clinical response platforms before a second pandemic (pdm) wave emerges.<h4>Methodology</h4>A total of 253 swab samples were collected from patients with influenza-like illness in the Osaka, Tokyo, and Chiba areas both in May 2009 and between October 2009 and January 2010. We analyzed partial sequences of the hemagglutinin (HA) and neuraminidase (NA) genes of the 2009 pdm influenza virus in the collected clinical samples. By phylogenetic analysis, we identified major variants of the 2009 pdm influenza virus and critical mutations associated with severe cases, including drug-resistance mutations.<h4>Results and conclusions</h4>Our sequence analysis has revealed that both HA-S220T and NA-N248D are major non-synonymous mutations that clearly discriminate the 2009 pdm influenza viruses identified in the very early phase (May 2009) from those found in the peak phase (October 2009 to January 2010) in Japan. By phylogenetic analysis, we found 14 micro-clades within the viruses collected during the peak phase. Among them, 12 were new micro-clades, while two were previously reported. Oseltamivir resistance-related mutations, i.e., NA-H275Y and NA-N295S, were also detected in sporadic cases in Osaka and Tokyo. |
| format |
article |
| author |
Jean-Étienne Morlighem Shintaro Aoki Mami Kishima Mitsue Hanami Chihiro Ogawa Amadu Jalloh Yukari Takahashi Yuki Kawai Satomi Saga Eiji Hayashi Toshiaki Ban Shinyu Izumi Akira Wada Masayuki Mano Megumu Fukunaga Yoshiyuki Kijima Masashi Shiomi Kaoru Inoue Takeshi Hata Yukihiro Koretsune Koichiro Kudo Yuji Himeno Aizan Hirai Kazuo Takahashi Yuko Sakai-Tagawa Kiyoko Iwatsuki-Horimoto Yoshihiro Kawaoka Yoshihide Hayashizaki Toshihisa Ishikawa |
| author_facet |
Jean-Étienne Morlighem Shintaro Aoki Mami Kishima Mitsue Hanami Chihiro Ogawa Amadu Jalloh Yukari Takahashi Yuki Kawai Satomi Saga Eiji Hayashi Toshiaki Ban Shinyu Izumi Akira Wada Masayuki Mano Megumu Fukunaga Yoshiyuki Kijima Masashi Shiomi Kaoru Inoue Takeshi Hata Yukihiro Koretsune Koichiro Kudo Yuji Himeno Aizan Hirai Kazuo Takahashi Yuko Sakai-Tagawa Kiyoko Iwatsuki-Horimoto Yoshihiro Kawaoka Yoshihide Hayashizaki Toshihisa Ishikawa |
| author_sort |
Jean-Étienne Morlighem |
| title |
Mutation analysis of 2009 pandemic influenza A(H1N1) viruses collected in Japan during the peak phase of the pandemic. |
| title_short |
Mutation analysis of 2009 pandemic influenza A(H1N1) viruses collected in Japan during the peak phase of the pandemic. |
| title_full |
Mutation analysis of 2009 pandemic influenza A(H1N1) viruses collected in Japan during the peak phase of the pandemic. |
| title_fullStr |
Mutation analysis of 2009 pandemic influenza A(H1N1) viruses collected in Japan during the peak phase of the pandemic. |
| title_full_unstemmed |
Mutation analysis of 2009 pandemic influenza A(H1N1) viruses collected in Japan during the peak phase of the pandemic. |
| title_sort |
mutation analysis of 2009 pandemic influenza a(h1n1) viruses collected in japan during the peak phase of the pandemic. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2011 |
| url |
https://doaj.org/article/723046f5766844e4a661c2cc58445948 |
| work_keys_str_mv |
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