Presence in the pre-surgical fine-needle aspiration of potential thyroid biomarkers previously identified in the post-surgical one.

Fine-needle aspiration biopsy (FNA) is usually applied to distinguish benign from malignant thyroid nodules. However, cytological analysis cannot always allow a proper diagnosis. We believe that the improvement of the diagnostic capability of pre-surgical FNA could avoid unnecessary thyroidectomy. I...

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Autores principales:	Federica Ciregia, Laura Giusti, Angelo Molinaro, Filippo Niccolai, Patrizia Agretti, Teresa Rago, Giancarlo Di Coscio, Paolo Vitti, Fulvio Basolo, Pietro Iacconi, Massimo Tonacchera, Antonio Lucacchini
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/723119af56eb47a790174069d080c80d
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spelling	oai:doaj.org-article:723119af56eb47a790174069d080c80d2021-11-18T08:57:23ZPresence in the pre-surgical fine-needle aspiration of potential thyroid biomarkers previously identified in the post-surgical one.1932-620310.1371/journal.pone.0072911https://doaj.org/article/723119af56eb47a790174069d080c80d2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24023790/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Fine-needle aspiration biopsy (FNA) is usually applied to distinguish benign from malignant thyroid nodules. However, cytological analysis cannot always allow a proper diagnosis. We believe that the improvement of the diagnostic capability of pre-surgical FNA could avoid unnecessary thyroidectomy. In a previous study, we performed a proteome analysis to examine FNA collected after thyroidectomy. With the present study, we examined the applicability of these results on pre-surgical FNA. We collected pre-surgical FNA from 411 consecutive patients, and to obtain a correct comparison with our previous results, we processed only benign (n=114), papillary classical variant (cPTC) (n=34) and papillary tall cell variant (TcPTC) (n=14) FNA. We evaluated levels of five proteins previously found up-regulated in thyroid cancer with respect to benign nodules. ELISA and western blot (WB) analysis were used to assay levels of L-lactate dehydrogenase B chain (LDHB), Ferritin heavy chain, Ferritin light chain, Annexin A1 (ANXA1), and Moesin in FNA. ELISA assays and WB analysis confirmed the increase of LDHB, Moesin, and ANXA1 in pre-surgical FNA of thyroid papillary cancer. Sensitivity and specificity of ANXA1 were respectively 87 and 94% for cPTC, 85 and 100% for TcPTC. In conclusion, a proteomic analysis of FNA from patients with thyroid nodules may help to distinguish benign versus malignant thyroid nodules. Moreover, ANXA1 appears to be an ideal candidate given the high sensitivity and specificity obtained from ROC curve analysis.Federica CiregiaLaura GiustiAngelo MolinaroFilippo NiccolaiPatrizia AgrettiTeresa RagoGiancarlo Di CoscioPaolo VittiFulvio BasoloPietro IacconiMassimo TonaccheraAntonio LucacchiniPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 9, p e72911 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Federica Ciregia Laura Giusti Angelo Molinaro Filippo Niccolai Patrizia Agretti Teresa Rago Giancarlo Di Coscio Paolo Vitti Fulvio Basolo Pietro Iacconi Massimo Tonacchera Antonio Lucacchini Presence in the pre-surgical fine-needle aspiration of potential thyroid biomarkers previously identified in the post-surgical one.
description	Fine-needle aspiration biopsy (FNA) is usually applied to distinguish benign from malignant thyroid nodules. However, cytological analysis cannot always allow a proper diagnosis. We believe that the improvement of the diagnostic capability of pre-surgical FNA could avoid unnecessary thyroidectomy. In a previous study, we performed a proteome analysis to examine FNA collected after thyroidectomy. With the present study, we examined the applicability of these results on pre-surgical FNA. We collected pre-surgical FNA from 411 consecutive patients, and to obtain a correct comparison with our previous results, we processed only benign (n=114), papillary classical variant (cPTC) (n=34) and papillary tall cell variant (TcPTC) (n=14) FNA. We evaluated levels of five proteins previously found up-regulated in thyroid cancer with respect to benign nodules. ELISA and western blot (WB) analysis were used to assay levels of L-lactate dehydrogenase B chain (LDHB), Ferritin heavy chain, Ferritin light chain, Annexin A1 (ANXA1), and Moesin in FNA. ELISA assays and WB analysis confirmed the increase of LDHB, Moesin, and ANXA1 in pre-surgical FNA of thyroid papillary cancer. Sensitivity and specificity of ANXA1 were respectively 87 and 94% for cPTC, 85 and 100% for TcPTC. In conclusion, a proteomic analysis of FNA from patients with thyroid nodules may help to distinguish benign versus malignant thyroid nodules. Moreover, ANXA1 appears to be an ideal candidate given the high sensitivity and specificity obtained from ROC curve analysis.
format	article
author	Federica Ciregia Laura Giusti Angelo Molinaro Filippo Niccolai Patrizia Agretti Teresa Rago Giancarlo Di Coscio Paolo Vitti Fulvio Basolo Pietro Iacconi Massimo Tonacchera Antonio Lucacchini
author_facet	Federica Ciregia Laura Giusti Angelo Molinaro Filippo Niccolai Patrizia Agretti Teresa Rago Giancarlo Di Coscio Paolo Vitti Fulvio Basolo Pietro Iacconi Massimo Tonacchera Antonio Lucacchini
author_sort	Federica Ciregia
title	Presence in the pre-surgical fine-needle aspiration of potential thyroid biomarkers previously identified in the post-surgical one.
title_short	Presence in the pre-surgical fine-needle aspiration of potential thyroid biomarkers previously identified in the post-surgical one.
title_full	Presence in the pre-surgical fine-needle aspiration of potential thyroid biomarkers previously identified in the post-surgical one.
title_fullStr	Presence in the pre-surgical fine-needle aspiration of potential thyroid biomarkers previously identified in the post-surgical one.
title_full_unstemmed	Presence in the pre-surgical fine-needle aspiration of potential thyroid biomarkers previously identified in the post-surgical one.
title_sort	presence in the pre-surgical fine-needle aspiration of potential thyroid biomarkers previously identified in the post-surgical one.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/723119af56eb47a790174069d080c80d
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Presence in the pre-surgical fine-needle aspiration of potential thyroid biomarkers previously identified in the post-surgical one.

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