Analysis of Parasitic Protozoa at the Single-cell Level using Microfluidic Impedance Cytometry

Abstract At present, there are few technologies which enable the detection, identification and viability analysis of protozoan pathogens including Cryptosporidium and/or Giardia at the single (oo)cyst level. We report the use of Microfluidic Impedance Cytometry (MIC) to characterise the AC electrica...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	J. S. McGrath, C. Honrado, D. Spencer, B. Horton, H. L. Bridle, H. Morgan
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/72332ed9a2614de78d0de5f5b8b23157
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:72332ed9a2614de78d0de5f5b8b23157
record_format	dspace
spelling	oai:doaj.org-article:72332ed9a2614de78d0de5f5b8b231572021-12-02T16:07:05ZAnalysis of Parasitic Protozoa at the Single-cell Level using Microfluidic Impedance Cytometry10.1038/s41598-017-02715-y2045-2322https://doaj.org/article/72332ed9a2614de78d0de5f5b8b231572017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02715-yhttps://doaj.org/toc/2045-2322Abstract At present, there are few technologies which enable the detection, identification and viability analysis of protozoan pathogens including Cryptosporidium and/or Giardia at the single (oo)cyst level. We report the use of Microfluidic Impedance Cytometry (MIC) to characterise the AC electrical (impedance) properties of single parasites and demonstrate rapid discrimination based on viability and species. Specifically, MIC was used to identify live and inactive C. parvum oocysts with over 90% certainty, whilst also detecting damaged and/or excysted oocysts. Furthermore, discrimination of Cryptosporidium parvum, Cryptosporidium muris and Giardia lamblia, with over 92% certainty was achieved. Enumeration and identification of (oo)cysts can be achieved in a few minutes, which offers a reduction in identification time and labour demands when compared to existing detection methods.J. S. McGrathC. HonradoD. SpencerB. HortonH. L. BridleH. MorganNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q J. S. McGrath C. Honrado D. Spencer B. Horton H. L. Bridle H. Morgan Analysis of Parasitic Protozoa at the Single-cell Level using Microfluidic Impedance Cytometry
description	Abstract At present, there are few technologies which enable the detection, identification and viability analysis of protozoan pathogens including Cryptosporidium and/or Giardia at the single (oo)cyst level. We report the use of Microfluidic Impedance Cytometry (MIC) to characterise the AC electrical (impedance) properties of single parasites and demonstrate rapid discrimination based on viability and species. Specifically, MIC was used to identify live and inactive C. parvum oocysts with over 90% certainty, whilst also detecting damaged and/or excysted oocysts. Furthermore, discrimination of Cryptosporidium parvum, Cryptosporidium muris and Giardia lamblia, with over 92% certainty was achieved. Enumeration and identification of (oo)cysts can be achieved in a few minutes, which offers a reduction in identification time and labour demands when compared to existing detection methods.
format	article
author	J. S. McGrath C. Honrado D. Spencer B. Horton H. L. Bridle H. Morgan
author_facet	J. S. McGrath C. Honrado D. Spencer B. Horton H. L. Bridle H. Morgan
author_sort	J. S. McGrath
title	Analysis of Parasitic Protozoa at the Single-cell Level using Microfluidic Impedance Cytometry
title_short	Analysis of Parasitic Protozoa at the Single-cell Level using Microfluidic Impedance Cytometry
title_full	Analysis of Parasitic Protozoa at the Single-cell Level using Microfluidic Impedance Cytometry
title_fullStr	Analysis of Parasitic Protozoa at the Single-cell Level using Microfluidic Impedance Cytometry
title_full_unstemmed	Analysis of Parasitic Protozoa at the Single-cell Level using Microfluidic Impedance Cytometry
title_sort	analysis of parasitic protozoa at the single-cell level using microfluidic impedance cytometry
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/72332ed9a2614de78d0de5f5b8b23157
work_keys_str_mv	AT jsmcgrath analysisofparasiticprotozoaatthesinglecelllevelusingmicrofluidicimpedancecytometry AT chonrado analysisofparasiticprotozoaatthesinglecelllevelusingmicrofluidicimpedancecytometry AT dspencer analysisofparasiticprotozoaatthesinglecelllevelusingmicrofluidicimpedancecytometry AT bhorton analysisofparasiticprotozoaatthesinglecelllevelusingmicrofluidicimpedancecytometry AT hlbridle analysisofparasiticprotozoaatthesinglecelllevelusingmicrofluidicimpedancecytometry AT hmorgan analysisofparasiticprotozoaatthesinglecelllevelusingmicrofluidicimpedancecytometry
_version_	1718384723328237568

Analysis of Parasitic Protozoa at the Single-cell Level using Microfluidic Impedance Cytometry

Ejemplares similares