METHODS OF ESTIMATION AND THE ROLE OF RESPIRATORY BURST IN THE PATHOGENESIS OF INFECTIOUS AND INFLAMMATORY DISEASES

According to the modern concepts the respiratory burst directly related to the processes of phagocytosis characterizes the functional activity of phagocytic cells. This review presents modern methods for assessing the respiratory burst state of phagocytes based on cytofluorometric and chemiluminesce...

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Autores principales:	A. A. Savchenko, I. V. Kudryavtsev, A. G. Borisov
Formato:	article
Lenguaje:	RU
Publicado:	Sankt-Peterburg : NIIÈM imeni Pastera 2018
Materias:	respiratory burst phagocytic cells neutrophils monocytes active oxygen species superoxide radical infectious and inflammatory diseases Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/723b8d4aaf8741f6b31a3308388d62d7
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spelling	oai:doaj.org-article:723b8d4aaf8741f6b31a3308388d62d72021-11-22T07:09:48ZMETHODS OF ESTIMATION AND THE ROLE OF RESPIRATORY BURST IN THE PATHOGENESIS OF INFECTIOUS AND INFLAMMATORY DISEASES2220-76192313-739810.15789/2220-7619-2017-4-327-340https://doaj.org/article/723b8d4aaf8741f6b31a3308388d62d72018-01-01T00:00:00Zhttps://www.iimmun.ru/iimm/article/view/590https://doaj.org/toc/2220-7619https://doaj.org/toc/2313-7398According to the modern concepts the respiratory burst directly related to the processes of phagocytosis characterizes the functional activity of phagocytic cells. This review presents modern methods for assessing the respiratory burst state of phagocytes based on cytofluorometric and chemiluminescent analysis. The sequence and mechanisms of the reactions of reactive oxygen species (ROS) synthesis in the process of respiratory cell burst are presented in detail. The sequence of synthesis from ROS with low bactericidal activity to ROS with high bactericidal activity is characterized. The review describes in detail the most popular dyes for cytofluorometric analysis to assess the levels of ROS synthesis. Characteristics and examples of the use of such dyes as dihydroethidine, dichlorodihydrofluorescein and dihydrorhodamine 123 are given. The main stages and mechanisms of the chemiluminescence reaction are presented. The features of the use of the main indicators (luminol and lucigenin) of the chemiluminescence reaction are described. A mechanism for estimating the parameters of the chemiluminescence reaction characterizing the features of the state and kinetics of the respiratory burst of phagocytic cells is given. The separate section of the review is devoted to the role of a respiratory burst in phagocytic cells in various immunopathological states. Data on the pathogenetic significance of changes in the intensity and kinetics of respiratory burst of phagocytes in infectious, inflammatory and oncological diseases were presented. Examples of new methods for diagnosing and predicting the course of the immunopathological states characters are presented on the basis of an assessment of the respiratory burst of phagocytic cells. The literature data show that at present, in the diagnosis and evaluation of the nature of the diseases characters the state of a respiratory burst is evaluated in various types of cells of innate immunity: neutrophils, monocytes, etc. It is concluded that the evaluation of the respiratory burst of phagocytic cells can be characterized as the fundamental mechanisms of reacting cells of innate immunity to pathogenic and regulatory effects, so to develop new highly sensitive methods for diagnosing and predicting the development and outcome of various immunopathological conditions. The presented methods of flow cytometry and chemiluminescence analysis make it possible to determine both the integral state of the respiratory explosion, and the levels and kinetic parameters of the synthesis of individual ROS.A. A. SavchenkoI. V. KudryavtsevA. G. BorisovSankt-Peterburg : NIIÈM imeni Pasteraarticlerespiratory burstphagocytic cellsneutrophilsmonocytesactive oxygen speciessuperoxide radicalinfectious and inflammatory diseasesInfectious and parasitic diseasesRC109-216RUInfekciâ i Immunitet, Vol 7, Iss 4, Pp 327-340 (2018)
institution	DOAJ
collection	DOAJ
language	RU
topic	respiratory burst phagocytic cells neutrophils monocytes active oxygen species superoxide radical infectious and inflammatory diseases Infectious and parasitic diseases RC109-216
spellingShingle	respiratory burst phagocytic cells neutrophils monocytes active oxygen species superoxide radical infectious and inflammatory diseases Infectious and parasitic diseases RC109-216 A. A. Savchenko I. V. Kudryavtsev A. G. Borisov METHODS OF ESTIMATION AND THE ROLE OF RESPIRATORY BURST IN THE PATHOGENESIS OF INFECTIOUS AND INFLAMMATORY DISEASES
description	According to the modern concepts the respiratory burst directly related to the processes of phagocytosis characterizes the functional activity of phagocytic cells. This review presents modern methods for assessing the respiratory burst state of phagocytes based on cytofluorometric and chemiluminescent analysis. The sequence and mechanisms of the reactions of reactive oxygen species (ROS) synthesis in the process of respiratory cell burst are presented in detail. The sequence of synthesis from ROS with low bactericidal activity to ROS with high bactericidal activity is characterized. The review describes in detail the most popular dyes for cytofluorometric analysis to assess the levels of ROS synthesis. Characteristics and examples of the use of such dyes as dihydroethidine, dichlorodihydrofluorescein and dihydrorhodamine 123 are given. The main stages and mechanisms of the chemiluminescence reaction are presented. The features of the use of the main indicators (luminol and lucigenin) of the chemiluminescence reaction are described. A mechanism for estimating the parameters of the chemiluminescence reaction characterizing the features of the state and kinetics of the respiratory burst of phagocytic cells is given. The separate section of the review is devoted to the role of a respiratory burst in phagocytic cells in various immunopathological states. Data on the pathogenetic significance of changes in the intensity and kinetics of respiratory burst of phagocytes in infectious, inflammatory and oncological diseases were presented. Examples of new methods for diagnosing and predicting the course of the immunopathological states characters are presented on the basis of an assessment of the respiratory burst of phagocytic cells. The literature data show that at present, in the diagnosis and evaluation of the nature of the diseases characters the state of a respiratory burst is evaluated in various types of cells of innate immunity: neutrophils, monocytes, etc. It is concluded that the evaluation of the respiratory burst of phagocytic cells can be characterized as the fundamental mechanisms of reacting cells of innate immunity to pathogenic and regulatory effects, so to develop new highly sensitive methods for diagnosing and predicting the development and outcome of various immunopathological conditions. The presented methods of flow cytometry and chemiluminescence analysis make it possible to determine both the integral state of the respiratory explosion, and the levels and kinetic parameters of the synthesis of individual ROS.
format	article
author	A. A. Savchenko I. V. Kudryavtsev A. G. Borisov
author_facet	A. A. Savchenko I. V. Kudryavtsev A. G. Borisov
author_sort	A. A. Savchenko
title	METHODS OF ESTIMATION AND THE ROLE OF RESPIRATORY BURST IN THE PATHOGENESIS OF INFECTIOUS AND INFLAMMATORY DISEASES
title_short	METHODS OF ESTIMATION AND THE ROLE OF RESPIRATORY BURST IN THE PATHOGENESIS OF INFECTIOUS AND INFLAMMATORY DISEASES
title_full	METHODS OF ESTIMATION AND THE ROLE OF RESPIRATORY BURST IN THE PATHOGENESIS OF INFECTIOUS AND INFLAMMATORY DISEASES
title_fullStr	METHODS OF ESTIMATION AND THE ROLE OF RESPIRATORY BURST IN THE PATHOGENESIS OF INFECTIOUS AND INFLAMMATORY DISEASES
title_full_unstemmed	METHODS OF ESTIMATION AND THE ROLE OF RESPIRATORY BURST IN THE PATHOGENESIS OF INFECTIOUS AND INFLAMMATORY DISEASES
title_sort	methods of estimation and the role of respiratory burst in the pathogenesis of infectious and inflammatory diseases
publisher	Sankt-Peterburg : NIIÈM imeni Pastera
publishDate	2018
url	https://doaj.org/article/723b8d4aaf8741f6b31a3308388d62d7
work_keys_str_mv	AT aasavchenko methodsofestimationandtheroleofrespiratoryburstinthepathogenesisofinfectiousandinflammatorydiseases AT ivkudryavtsev methodsofestimationandtheroleofrespiratoryburstinthepathogenesisofinfectiousandinflammatorydiseases AT agborisov methodsofestimationandtheroleofrespiratoryburstinthepathogenesisofinfectiousandinflammatorydiseases
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METHODS OF ESTIMATION AND THE ROLE OF RESPIRATORY BURST IN THE PATHOGENESIS OF INFECTIOUS AND INFLAMMATORY DISEASES

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