Hookworm treatment induces a decrease of suppressive regulatory T cell associated with a Th2 inflammatory response.

<h4>Background</h4>Like other helminths, hookworms (HW) induce a regulatory immune response able to modulate and dampen reactivity of the host to antigens. No data about the evolution of the immune response after treatment are available. We aim to phenotype the regulatory immune response...

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Autores principales:	Virginie Doyen, Francis Corazza, Hoa Nhu Thi, Thanh Le Chi, Carine Truyens, Carole Nagant, Hiep Tran Thi Mong, Jean-Francois Fils, Phuong Thi Ngoc Huynh, Olivier Michel
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/723e69551f2748578661a37d2edc09aa
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spelling	oai:doaj.org-article:723e69551f2748578661a37d2edc09aa2021-12-02T20:10:49ZHookworm treatment induces a decrease of suppressive regulatory T cell associated with a Th2 inflammatory response.1932-620310.1371/journal.pone.0252921https://doaj.org/article/723e69551f2748578661a37d2edc09aa2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0252921https://doaj.org/toc/1932-6203<h4>Background</h4>Like other helminths, hookworms (HW) induce a regulatory immune response able to modulate and dampen reactivity of the host to antigens. No data about the evolution of the immune response after treatment are available. We aim to phenotype the regulatory immune response during natural HW infection and its evolution after treatment.<h4>Methodology</h4>Twenty hookworm infected (HW+) and 14 non-infected subjects HW-from endemic area in the periphery of Ho Chi Minh City were included. Blood and feces samples were obtained before, 2 and 4 weeks after treatment with Albendazole 400mg. Additional samples were obtained at 3 and 12 months in the HW+ group. Hematological parameters, Treg (CD4+CD25hiFoxP3hi) and surface molecules (CD39, CD62L, ICOS, PD-1, CD45RA) were measured as well as inflammatory and lymphocytes differentiation cytokines such as IL-1β, IL-6, IFNγ, IL-4, IL-17, IL-10, IL-2 and TGFβ.<h4>Results</h4>HW+ subjects showed higher Treg, TregICOS+, Treg PD1-, TregCD62L+ and CD45RA+FoxP3lo resting Treg (rTreg). CD45RA-FoxP3lo non-suppressive Treg cells were also increased. No preferential Th1/Th2 orientation was observed, nor difference for IL-10 between two groups. After treatment, Treg, TregICOS+, TregCD62L+, Treg PD1- and rTreg decreased while IL-4 and IL-6 cytokines increased.<h4>Conclusion</h4>During HW infection, Treg are increased and characterized by a heterogeneous population: a highly suppressive as well as a non-suppressive T cells phenotype. After treatment, Treg with immune-suppressive phenotype exhibited a decrease parallel to an inflammatory Th2 response.Virginie DoyenFrancis CorazzaHoa Nhu ThiThanh Le ChiCarine TruyensCarole NagantHiep Tran Thi MongJean-Francois FilsPhuong Thi Ngoc HuynhOlivier MichelPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 6, p e0252921 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Virginie Doyen Francis Corazza Hoa Nhu Thi Thanh Le Chi Carine Truyens Carole Nagant Hiep Tran Thi Mong Jean-Francois Fils Phuong Thi Ngoc Huynh Olivier Michel Hookworm treatment induces a decrease of suppressive regulatory T cell associated with a Th2 inflammatory response.
description	<h4>Background</h4>Like other helminths, hookworms (HW) induce a regulatory immune response able to modulate and dampen reactivity of the host to antigens. No data about the evolution of the immune response after treatment are available. We aim to phenotype the regulatory immune response during natural HW infection and its evolution after treatment.<h4>Methodology</h4>Twenty hookworm infected (HW+) and 14 non-infected subjects HW-from endemic area in the periphery of Ho Chi Minh City were included. Blood and feces samples were obtained before, 2 and 4 weeks after treatment with Albendazole 400mg. Additional samples were obtained at 3 and 12 months in the HW+ group. Hematological parameters, Treg (CD4+CD25hiFoxP3hi) and surface molecules (CD39, CD62L, ICOS, PD-1, CD45RA) were measured as well as inflammatory and lymphocytes differentiation cytokines such as IL-1β, IL-6, IFNγ, IL-4, IL-17, IL-10, IL-2 and TGFβ.<h4>Results</h4>HW+ subjects showed higher Treg, TregICOS+, Treg PD1-, TregCD62L+ and CD45RA+FoxP3lo resting Treg (rTreg). CD45RA-FoxP3lo non-suppressive Treg cells were also increased. No preferential Th1/Th2 orientation was observed, nor difference for IL-10 between two groups. After treatment, Treg, TregICOS+, TregCD62L+, Treg PD1- and rTreg decreased while IL-4 and IL-6 cytokines increased.<h4>Conclusion</h4>During HW infection, Treg are increased and characterized by a heterogeneous population: a highly suppressive as well as a non-suppressive T cells phenotype. After treatment, Treg with immune-suppressive phenotype exhibited a decrease parallel to an inflammatory Th2 response.
format	article
author	Virginie Doyen Francis Corazza Hoa Nhu Thi Thanh Le Chi Carine Truyens Carole Nagant Hiep Tran Thi Mong Jean-Francois Fils Phuong Thi Ngoc Huynh Olivier Michel
author_facet	Virginie Doyen Francis Corazza Hoa Nhu Thi Thanh Le Chi Carine Truyens Carole Nagant Hiep Tran Thi Mong Jean-Francois Fils Phuong Thi Ngoc Huynh Olivier Michel
author_sort	Virginie Doyen
title	Hookworm treatment induces a decrease of suppressive regulatory T cell associated with a Th2 inflammatory response.
title_short	Hookworm treatment induces a decrease of suppressive regulatory T cell associated with a Th2 inflammatory response.
title_full	Hookworm treatment induces a decrease of suppressive regulatory T cell associated with a Th2 inflammatory response.
title_fullStr	Hookworm treatment induces a decrease of suppressive regulatory T cell associated with a Th2 inflammatory response.
title_full_unstemmed	Hookworm treatment induces a decrease of suppressive regulatory T cell associated with a Th2 inflammatory response.
title_sort	hookworm treatment induces a decrease of suppressive regulatory t cell associated with a th2 inflammatory response.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/723e69551f2748578661a37d2edc09aa
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Hookworm treatment induces a decrease of suppressive regulatory T cell associated with a Th2 inflammatory response.

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