Arabidopsis bHLH100 and bHLH101 control iron homeostasis via a FIT-independent pathway.

Iron deficiency induces a complex set of responses in plants, including developmental and physiological changes, to increase iron uptake from soil. In Arabidopsis, many transporters involved in the absorption and distribution of iron have been identified over the past decade. However, little is know...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Alicia B Sivitz, Victor Hermand, Catherine Curie, Grégory Vert
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/723f97f7f43d4ce69082ad4820d9ca00
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:723f97f7f43d4ce69082ad4820d9ca00
record_format	dspace
spelling	oai:doaj.org-article:723f97f7f43d4ce69082ad4820d9ca002021-11-18T07:06:00ZArabidopsis bHLH100 and bHLH101 control iron homeostasis via a FIT-independent pathway.1932-620310.1371/journal.pone.0044843https://doaj.org/article/723f97f7f43d4ce69082ad4820d9ca002012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22984573/?tool=EBIhttps://doaj.org/toc/1932-6203Iron deficiency induces a complex set of responses in plants, including developmental and physiological changes, to increase iron uptake from soil. In Arabidopsis, many transporters involved in the absorption and distribution of iron have been identified over the past decade. However, little is known about the signaling pathways and networks driving the various responses to low iron. Only the basic helix-loop-helix (bHLH) transcription factor FIT has been shown to control the expression of the root iron uptake machinery genes FRO2 and IRT1. Here, we characterize the biological role of two other iron-regulated transcription factors, bHLH100 and bHLH101, in iron homeostasis. First direct transcriptional targets of FIT were determined in vivo. We show that bHLH100 and bHLH101 do not regulate FIT target genes, suggesting that they play a non-redundant role with the two closely related bHLH factors bHLH038 and bHLH039 that have been suggested to act in concert with FIT. bHLH100 and bHLH101 play a crucial role in iron-deficiency responses, as attested by their severe growth defects and iron homeostasis related phenotypes on low-iron media. To gain further insight into the biological role of bHLH100 and bHLH101, we performed microarray analysis using the corresponding double mutant and showed that bHLH100 and bHLH101 likely regulate genes involved in the distribution of iron within the plant. Altogether, this work establishes bHLH100 and bHLH101 as key regulators of iron-deficiency responses independent of the master regulator FIT and sheds light on new regulatory networks important for proper growth and development under low iron conditions.Alicia B SivitzVictor HermandCatherine CurieGrégory VertPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 9, p e44843 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Alicia B Sivitz Victor Hermand Catherine Curie Grégory Vert Arabidopsis bHLH100 and bHLH101 control iron homeostasis via a FIT-independent pathway.
description	Iron deficiency induces a complex set of responses in plants, including developmental and physiological changes, to increase iron uptake from soil. In Arabidopsis, many transporters involved in the absorption and distribution of iron have been identified over the past decade. However, little is known about the signaling pathways and networks driving the various responses to low iron. Only the basic helix-loop-helix (bHLH) transcription factor FIT has been shown to control the expression of the root iron uptake machinery genes FRO2 and IRT1. Here, we characterize the biological role of two other iron-regulated transcription factors, bHLH100 and bHLH101, in iron homeostasis. First direct transcriptional targets of FIT were determined in vivo. We show that bHLH100 and bHLH101 do not regulate FIT target genes, suggesting that they play a non-redundant role with the two closely related bHLH factors bHLH038 and bHLH039 that have been suggested to act in concert with FIT. bHLH100 and bHLH101 play a crucial role in iron-deficiency responses, as attested by their severe growth defects and iron homeostasis related phenotypes on low-iron media. To gain further insight into the biological role of bHLH100 and bHLH101, we performed microarray analysis using the corresponding double mutant and showed that bHLH100 and bHLH101 likely regulate genes involved in the distribution of iron within the plant. Altogether, this work establishes bHLH100 and bHLH101 as key regulators of iron-deficiency responses independent of the master regulator FIT and sheds light on new regulatory networks important for proper growth and development under low iron conditions.
format	article
author	Alicia B Sivitz Victor Hermand Catherine Curie Grégory Vert
author_facet	Alicia B Sivitz Victor Hermand Catherine Curie Grégory Vert
author_sort	Alicia B Sivitz
title	Arabidopsis bHLH100 and bHLH101 control iron homeostasis via a FIT-independent pathway.
title_short	Arabidopsis bHLH100 and bHLH101 control iron homeostasis via a FIT-independent pathway.
title_full	Arabidopsis bHLH100 and bHLH101 control iron homeostasis via a FIT-independent pathway.
title_fullStr	Arabidopsis bHLH100 and bHLH101 control iron homeostasis via a FIT-independent pathway.
title_full_unstemmed	Arabidopsis bHLH100 and bHLH101 control iron homeostasis via a FIT-independent pathway.
title_sort	arabidopsis bhlh100 and bhlh101 control iron homeostasis via a fit-independent pathway.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/723f97f7f43d4ce69082ad4820d9ca00
work_keys_str_mv	AT aliciabsivitz arabidopsisbhlh100andbhlh101controlironhomeostasisviaafitindependentpathway AT victorhermand arabidopsisbhlh100andbhlh101controlironhomeostasisviaafitindependentpathway AT catherinecurie arabidopsisbhlh100andbhlh101controlironhomeostasisviaafitindependentpathway AT gregoryvert arabidopsisbhlh100andbhlh101controlironhomeostasisviaafitindependentpathway
_version_	1718423921490919424

Arabidopsis bHLH100 and bHLH101 control iron homeostasis via a FIT-independent pathway.

Ejemplares similares