Human papillomavirus genotype distribution in Czech women and men with diseases etiologically linked to HPV.

<h4>Background</h4>The HPV prevalence and genotype distribution are important for the estimation of the impact of HPV-based cervical cancer screening and HPV vaccination on the incidence of diseases etiologically linked to HPVs. The HPV genotype distribution varies across different geogr...

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Autores principales:	Ruth Tachezy, Jana Smahelova, Martina Salakova, Marc Arbyn, Lukas Rob, Petr Skapa, Tomas Jirasek, Eva Hamsikova
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7241288b61f34bcbb09644ea1dad9268
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spelling	oai:doaj.org-article:7241288b61f34bcbb09644ea1dad92682021-11-18T06:50:21ZHuman papillomavirus genotype distribution in Czech women and men with diseases etiologically linked to HPV.1932-620310.1371/journal.pone.0021913https://doaj.org/article/7241288b61f34bcbb09644ea1dad92682011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21765924/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>The HPV prevalence and genotype distribution are important for the estimation of the impact of HPV-based cervical cancer screening and HPV vaccination on the incidence of diseases etiologically linked to HPVs. The HPV genotype distribution varies across different geographical regions. Therefore, we investigated the type-specific HPV prevalence in Czech women and men with anogenital diseases.<h4>Methods</h4>We analyzed 157 squamous cell carcinoma samples, 695 precancerous lesion samples and 64 cervical, vulvar and anal condylomata acuminate samples. HPV detection and typing were performed by PCR with GP5+/6+ primers, reverse line blot assay and sequencing.<h4>Results</h4>Thirty different HPV genotypes were detected in our study, HPV 16 being the most prevalent type both in precancerous lesions (45%) and squamous cell carcinomas (59%). In benign lesions, HPV 6 (72%) was the most common type. Altogether, 61% of carcinoma samples and 43% of precancerous lesion samples contained HPV 16 and/or 18. The presence of HPV types related to the vaccinal ones (HPV 31, 45, 33, 52, 58) were detected in 16% of carcinoma samples and 18% of precancerous lesion samples. HPV 16 and/or 18 were present in 76% of cervical cancer samples, 33% of CIN1, 43% CIN2 and 71% of CIN3 samples. HPV types 6 and/or 11 were detected in 84% samples of condylomata acuminate samples.<h4>Conclusions</h4>The prevalence of vaccinal and related HPV types in patients with HPV-associated diseases in the Czech Republic is very high. We may assume that the implementation of routine vaccination against HPV would greatly reduce the burden of HPV-associated diseases in the Czech Republic.Ruth TachezyJana SmahelovaMartina SalakovaMarc ArbynLukas RobPetr SkapaTomas JirasekEva HamsikovaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 7, p e21913 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ruth Tachezy Jana Smahelova Martina Salakova Marc Arbyn Lukas Rob Petr Skapa Tomas Jirasek Eva Hamsikova Human papillomavirus genotype distribution in Czech women and men with diseases etiologically linked to HPV.
description	<h4>Background</h4>The HPV prevalence and genotype distribution are important for the estimation of the impact of HPV-based cervical cancer screening and HPV vaccination on the incidence of diseases etiologically linked to HPVs. The HPV genotype distribution varies across different geographical regions. Therefore, we investigated the type-specific HPV prevalence in Czech women and men with anogenital diseases.<h4>Methods</h4>We analyzed 157 squamous cell carcinoma samples, 695 precancerous lesion samples and 64 cervical, vulvar and anal condylomata acuminate samples. HPV detection and typing were performed by PCR with GP5+/6+ primers, reverse line blot assay and sequencing.<h4>Results</h4>Thirty different HPV genotypes were detected in our study, HPV 16 being the most prevalent type both in precancerous lesions (45%) and squamous cell carcinomas (59%). In benign lesions, HPV 6 (72%) was the most common type. Altogether, 61% of carcinoma samples and 43% of precancerous lesion samples contained HPV 16 and/or 18. The presence of HPV types related to the vaccinal ones (HPV 31, 45, 33, 52, 58) were detected in 16% of carcinoma samples and 18% of precancerous lesion samples. HPV 16 and/or 18 were present in 76% of cervical cancer samples, 33% of CIN1, 43% CIN2 and 71% of CIN3 samples. HPV types 6 and/or 11 were detected in 84% samples of condylomata acuminate samples.<h4>Conclusions</h4>The prevalence of vaccinal and related HPV types in patients with HPV-associated diseases in the Czech Republic is very high. We may assume that the implementation of routine vaccination against HPV would greatly reduce the burden of HPV-associated diseases in the Czech Republic.
format	article
author	Ruth Tachezy Jana Smahelova Martina Salakova Marc Arbyn Lukas Rob Petr Skapa Tomas Jirasek Eva Hamsikova
author_facet	Ruth Tachezy Jana Smahelova Martina Salakova Marc Arbyn Lukas Rob Petr Skapa Tomas Jirasek Eva Hamsikova
author_sort	Ruth Tachezy
title	Human papillomavirus genotype distribution in Czech women and men with diseases etiologically linked to HPV.
title_short	Human papillomavirus genotype distribution in Czech women and men with diseases etiologically linked to HPV.
title_full	Human papillomavirus genotype distribution in Czech women and men with diseases etiologically linked to HPV.
title_fullStr	Human papillomavirus genotype distribution in Czech women and men with diseases etiologically linked to HPV.
title_full_unstemmed	Human papillomavirus genotype distribution in Czech women and men with diseases etiologically linked to HPV.
title_sort	human papillomavirus genotype distribution in czech women and men with diseases etiologically linked to hpv.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/7241288b61f34bcbb09644ea1dad9268
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Human papillomavirus genotype distribution in Czech women and men with diseases etiologically linked to HPV.

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