Mycosubtilin Produced by Bacillus subtilis ATCC6633 Inhibits Growth and Mycotoxin Biosynthesis of Fusarium graminearum and Fusarium verticillioides

Fusarium graminearum and Fusarium verticillioides are fungal pathogens that cause diseases in cereal crops, such as Fusarium head blight (FHB), seedling blight, and stalk rot. They also produce a variety of mycotoxins that reduce crop yields and threaten human a...

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Autores principales:	Chenjie Yu, Xin Liu, Xinyue Zhang, Mengxuan Zhang, Yiying Gu, Qurban Ali, M. Sherif Ramzy Mohamed, Jianhong Xu, Jianrong Shi, Xuewen Gao, Huijun Wu, Qin Gu
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	mycosubtilin <i>Bacillus subtilis</i> <i>Fusarium graminearum</i> <i>Fusarium verticillioides</i> biocontrol mycotoxins Medicine R
Acceso en línea:	https://doaj.org/article/7244a9f0aa1c4e51a93698d8c9cf8d74
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spelling	oai:doaj.org-article:7244a9f0aa1c4e51a93698d8c9cf8d742021-11-25T19:08:52ZMycosubtilin Produced by <i>Bacillus subtilis</i> ATCC6633 Inhibits Growth and Mycotoxin Biosynthesis of <i>Fusarium graminearum</i> and <i>Fusarium verticillioides</i>10.3390/toxins131107912072-6651https://doaj.org/article/7244a9f0aa1c4e51a93698d8c9cf8d742021-11-01T00:00:00Zhttps://www.mdpi.com/2072-6651/13/11/791https://doaj.org/toc/2072-6651<i>Fusarium graminearum</i> and <i>Fusarium verticillioides</i> are fungal pathogens that cause diseases in cereal crops, such as Fusarium head blight (FHB), seedling blight, and stalk rot. They also produce a variety of mycotoxins that reduce crop yields and threaten human and animal health. Several strategies for controlling these diseases have been developed. However, due to a lack of resistant cultivars and the hazards of chemical fungicides, efforts are now focused on the biocontrol of plant diseases, which is a more sustainable and environmentally friendly approach. In the present study, the lipopeptide mycosubtilin purified from <i>Bacillus subtilis</i> ATCC6633 significantly suppressed the growth of <i>F. graminearum</i> PH-1 and <i>F. verticillioides</i> 7600 in vitro. Mycosubtilin caused the destruction and deformation of plasma membranes and cell walls in <i>F. graminearum</i> hyphae. Additionally, mycosubtilin inhibited conidial spore formation and germination of both fungi in a dose-dependent manner. <i>In planta</i> experiments demonstrated the ability of mycosubtilin to control the adverse effects caused by <i>F. graminearum</i> and <i>F. verticillioides</i> on wheat heads and maize kernels<i>,</i> respectively. Mycosubtilin significantly decreased the production of deoxynivalenol (DON) and B-series fumonisins (FB<sub>1</sub>, FB<sub>2</sub> and FB<sub>3</sub>) in infected grains, with inhibition rates of 48.92, 48.48, 52.42, and 59.44%, respectively. The qRT-PCR analysis showed that mycosubtilin significantly downregulated genes involved in mycotoxin biosynthesis. In conclusion, mycosubtilin produced by <i>B</i><i>. subtilis</i> ATCC6633 was shown to have potential as a biological agent to control plant diseases and <i>Fusarium</i> toxin contamination caused by <i>F. graminearum</i> and <i>F. verticillioides.</i>Chenjie YuXin LiuXinyue ZhangMengxuan ZhangYiying GuQurban AliM. Sherif Ramzy MohamedJianhong XuJianrong ShiXuewen GaoHuijun WuQin GuMDPI AGarticlemycosubtilin<i>Bacillus subtilis</i><i>Fusarium graminearum</i><i>Fusarium verticillioides</i>biocontrolmycotoxinsMedicineRENToxins, Vol 13, Iss 791, p 791 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	mycosubtilin <i>Bacillus subtilis</i> <i>Fusarium graminearum</i> <i>Fusarium verticillioides</i> biocontrol mycotoxins Medicine R
spellingShingle	mycosubtilin <i>Bacillus subtilis</i> <i>Fusarium graminearum</i> <i>Fusarium verticillioides</i> biocontrol mycotoxins Medicine R Chenjie Yu Xin Liu Xinyue Zhang Mengxuan Zhang Yiying Gu Qurban Ali M. Sherif Ramzy Mohamed Jianhong Xu Jianrong Shi Xuewen Gao Huijun Wu Qin Gu Mycosubtilin Produced by <i>Bacillus subtilis</i> ATCC6633 Inhibits Growth and Mycotoxin Biosynthesis of <i>Fusarium graminearum</i> and <i>Fusarium verticillioides</i>
description	<i>Fusarium graminearum</i> and <i>Fusarium verticillioides</i> are fungal pathogens that cause diseases in cereal crops, such as Fusarium head blight (FHB), seedling blight, and stalk rot. They also produce a variety of mycotoxins that reduce crop yields and threaten human and animal health. Several strategies for controlling these diseases have been developed. However, due to a lack of resistant cultivars and the hazards of chemical fungicides, efforts are now focused on the biocontrol of plant diseases, which is a more sustainable and environmentally friendly approach. In the present study, the lipopeptide mycosubtilin purified from <i>Bacillus subtilis</i> ATCC6633 significantly suppressed the growth of <i>F. graminearum</i> PH-1 and <i>F. verticillioides</i> 7600 in vitro. Mycosubtilin caused the destruction and deformation of plasma membranes and cell walls in <i>F. graminearum</i> hyphae. Additionally, mycosubtilin inhibited conidial spore formation and germination of both fungi in a dose-dependent manner. <i>In planta</i> experiments demonstrated the ability of mycosubtilin to control the adverse effects caused by <i>F. graminearum</i> and <i>F. verticillioides</i> on wheat heads and maize kernels<i>,</i> respectively. Mycosubtilin significantly decreased the production of deoxynivalenol (DON) and B-series fumonisins (FB<sub>1</sub>, FB<sub>2</sub> and FB<sub>3</sub>) in infected grains, with inhibition rates of 48.92, 48.48, 52.42, and 59.44%, respectively. The qRT-PCR analysis showed that mycosubtilin significantly downregulated genes involved in mycotoxin biosynthesis. In conclusion, mycosubtilin produced by <i>B</i><i>. subtilis</i> ATCC6633 was shown to have potential as a biological agent to control plant diseases and <i>Fusarium</i> toxin contamination caused by <i>F. graminearum</i> and <i>F. verticillioides.</i>
format	article
author	Chenjie Yu Xin Liu Xinyue Zhang Mengxuan Zhang Yiying Gu Qurban Ali M. Sherif Ramzy Mohamed Jianhong Xu Jianrong Shi Xuewen Gao Huijun Wu Qin Gu
author_facet	Chenjie Yu Xin Liu Xinyue Zhang Mengxuan Zhang Yiying Gu Qurban Ali M. Sherif Ramzy Mohamed Jianhong Xu Jianrong Shi Xuewen Gao Huijun Wu Qin Gu
author_sort	Chenjie Yu
title	Mycosubtilin Produced by <i>Bacillus subtilis</i> ATCC6633 Inhibits Growth and Mycotoxin Biosynthesis of <i>Fusarium graminearum</i> and <i>Fusarium verticillioides</i>
title_short	Mycosubtilin Produced by <i>Bacillus subtilis</i> ATCC6633 Inhibits Growth and Mycotoxin Biosynthesis of <i>Fusarium graminearum</i> and <i>Fusarium verticillioides</i>
title_full	Mycosubtilin Produced by <i>Bacillus subtilis</i> ATCC6633 Inhibits Growth and Mycotoxin Biosynthesis of <i>Fusarium graminearum</i> and <i>Fusarium verticillioides</i>
title_fullStr	Mycosubtilin Produced by <i>Bacillus subtilis</i> ATCC6633 Inhibits Growth and Mycotoxin Biosynthesis of <i>Fusarium graminearum</i> and <i>Fusarium verticillioides</i>
title_full_unstemmed	Mycosubtilin Produced by <i>Bacillus subtilis</i> ATCC6633 Inhibits Growth and Mycotoxin Biosynthesis of <i>Fusarium graminearum</i> and <i>Fusarium verticillioides</i>
title_sort	mycosubtilin produced by <i>bacillus subtilis</i> atcc6633 inhibits growth and mycotoxin biosynthesis of <i>fusarium graminearum</i> and <i>fusarium verticillioides</i>
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/7244a9f0aa1c4e51a93698d8c9cf8d74
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Mycosubtilin Produced by <i>Bacillus subtilis</i> ATCC6633 Inhibits Growth and Mycotoxin Biosynthesis of <i>Fusarium graminearum</i> and <i>Fusarium verticillioides</i>

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