Transcriptome analysis in LRRK2 and idiopathic Parkinson’s disease at different glucose levels

Abstract Type-2 diabetes (T2D) and glucose metabolic imbalances have been linked to neurodegenerative diseases, including Parkinson’s disease (PD). To detect potential effects of different glucose levels on gene expression, by RNA-seq we analyzed the transcriptome of dermal fibroblasts from idiopath...

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Autores principales:	Rubén Fernández-Santiago, Anna Esteve-Codina, Manel Fernández, Francesc Valldeoriola, Almudena Sanchez-Gómez, Esteban Muñoz, Yaroslau Compta, Eduardo Tolosa, Mario Ezquerra, María J. Martí
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Neurology. Diseases of the nervous system RC346-429
Acceso en línea:	https://doaj.org/article/7246e0b9640e48e58c03a535ba5224ab
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spelling	oai:doaj.org-article:7246e0b9640e48e58c03a535ba5224ab2021-12-05T12:17:25ZTranscriptome analysis in LRRK2 and idiopathic Parkinson’s disease at different glucose levels10.1038/s41531-021-00255-x2373-8057https://doaj.org/article/7246e0b9640e48e58c03a535ba5224ab2021-12-01T00:00:00Zhttps://doi.org/10.1038/s41531-021-00255-xhttps://doaj.org/toc/2373-8057Abstract Type-2 diabetes (T2D) and glucose metabolic imbalances have been linked to neurodegenerative diseases, including Parkinson’s disease (PD). To detect potential effects of different glucose levels on gene expression, by RNA-seq we analyzed the transcriptome of dermal fibroblasts from idiopathic PD (iPD) patients, LRRK2-associated PD (L2PD) patients, and healthy controls (total n = 21 cell lines), which were cultured at two different glucose concentrations (25 and 5 mM glucose). In PD patients we identified differentially expressed genes (DEGs) that were related to biological processes mainly involving the plasmatic cell membrane, the extracellular matrix, and also neuronal functions. Such pathway deregulation was largely similar in iPD or L2PD fibroblasts. Overall, the gene expression changes detected in this study were associated with PD independently of glucose concentration.Rubén Fernández-SantiagoAnna Esteve-CodinaManel FernándezFrancesc ValldeoriolaAlmudena Sanchez-GómezEsteban MuñozYaroslau ComptaEduardo TolosaMario EzquerraMaría J. MartíNature PortfolioarticleNeurology. Diseases of the nervous systemRC346-429ENnpj Parkinson's Disease, Vol 7, Iss 1, Pp 1-7 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Neurology. Diseases of the nervous system RC346-429
spellingShingle	Neurology. Diseases of the nervous system RC346-429 Rubén Fernández-Santiago Anna Esteve-Codina Manel Fernández Francesc Valldeoriola Almudena Sanchez-Gómez Esteban Muñoz Yaroslau Compta Eduardo Tolosa Mario Ezquerra María J. Martí Transcriptome analysis in LRRK2 and idiopathic Parkinson’s disease at different glucose levels
description	Abstract Type-2 diabetes (T2D) and glucose metabolic imbalances have been linked to neurodegenerative diseases, including Parkinson’s disease (PD). To detect potential effects of different glucose levels on gene expression, by RNA-seq we analyzed the transcriptome of dermal fibroblasts from idiopathic PD (iPD) patients, LRRK2-associated PD (L2PD) patients, and healthy controls (total n = 21 cell lines), which were cultured at two different glucose concentrations (25 and 5 mM glucose). In PD patients we identified differentially expressed genes (DEGs) that were related to biological processes mainly involving the plasmatic cell membrane, the extracellular matrix, and also neuronal functions. Such pathway deregulation was largely similar in iPD or L2PD fibroblasts. Overall, the gene expression changes detected in this study were associated with PD independently of glucose concentration.
format	article
author	Rubén Fernández-Santiago Anna Esteve-Codina Manel Fernández Francesc Valldeoriola Almudena Sanchez-Gómez Esteban Muñoz Yaroslau Compta Eduardo Tolosa Mario Ezquerra María J. Martí
author_facet	Rubén Fernández-Santiago Anna Esteve-Codina Manel Fernández Francesc Valldeoriola Almudena Sanchez-Gómez Esteban Muñoz Yaroslau Compta Eduardo Tolosa Mario Ezquerra María J. Martí
author_sort	Rubén Fernández-Santiago
title	Transcriptome analysis in LRRK2 and idiopathic Parkinson’s disease at different glucose levels
title_short	Transcriptome analysis in LRRK2 and idiopathic Parkinson’s disease at different glucose levels
title_full	Transcriptome analysis in LRRK2 and idiopathic Parkinson’s disease at different glucose levels
title_fullStr	Transcriptome analysis in LRRK2 and idiopathic Parkinson’s disease at different glucose levels
title_full_unstemmed	Transcriptome analysis in LRRK2 and idiopathic Parkinson’s disease at different glucose levels
title_sort	transcriptome analysis in lrrk2 and idiopathic parkinson’s disease at different glucose levels
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/7246e0b9640e48e58c03a535ba5224ab
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Transcriptome analysis in LRRK2 and idiopathic Parkinson’s disease at different glucose levels

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