Global Profiling of N-Glycoproteins and N-Glycans in the Diatom Phaeodactylum tricornutum

N-glycosylation is an important posttranslational modification in all eukaryotes, but little is known about the N-glycoproteins and N-glycans in microalgae. Here, N-glycoproteomic and N-glycomic approaches were used to unveil the N-glycoproteins and N-glycans in the model diatom Phaeodactylum tricor...

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Autores principales:	Xihui Xie, Hong Du, Jichen Chen, Muhammad Aslam, Wanna Wang, Weizhou Chen, Ping Li, Hua Du, Xiaojuan Liu
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	N-glycosylation modification N-glycoprotein N-glycan Phaeodactylum tricornutum biopharmaceuticals Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/724e17d247a34a2893d3af0f1385b6fa
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spelling	oai:doaj.org-article:724e17d247a34a2893d3af0f1385b6fa2021-12-03T06:36:09ZGlobal Profiling of N-Glycoproteins and N-Glycans in the Diatom Phaeodactylum tricornutum1664-462X10.3389/fpls.2021.779307https://doaj.org/article/724e17d247a34a2893d3af0f1385b6fa2021-12-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fpls.2021.779307/fullhttps://doaj.org/toc/1664-462XN-glycosylation is an important posttranslational modification in all eukaryotes, but little is known about the N-glycoproteins and N-glycans in microalgae. Here, N-glycoproteomic and N-glycomic approaches were used to unveil the N-glycoproteins and N-glycans in the model diatom Phaeodactylum tricornutum. In total, 863 different N-glycopeptides corresponding to 639 N-glycoproteins were identified from P. tricornutum. These N-glycoproteins participated in a variety of important metabolic pathways in P. tricornutum. Twelve proteins participating in the N-glycosylation pathway were identified as N-glycoproteins, indicating that the N-glycosylation of these proteins might be important for the protein N-glycosylation pathway. Subsequently, 69 N-glycans corresponding to 59 N-glycoproteins were identified and classified into high mannose and hybrid type N-glycans. High mannose type N-glycans contained four different classes, such as Man-5, Man-7, Man-9, and Man-10 with a terminal glucose residue. Hybrid type N-glycan harbored Man-4 with a terminal GlcNAc residue. The identification of N-glycosylation on nascent proteins expanded our understanding of this modification at a N-glycoproteomic scale, the analysis of N-glycan structures updated the N-glycan database in microalgae. The results obtained from this study facilitate the elucidation of the precise function of these N-glycoproteins and are beneficial for future designing the microalga to produce the functional humanized biopharmaceutical N-glycoproteins for the clinical therapeutics.Xihui XieXihui XieHong DuHong DuJichen ChenJichen ChenMuhammad AslamMuhammad AslamMuhammad AslamWanna WangWanna WangWeizhou ChenWeizhou ChenPing LiHua DuHua DuXiaojuan LiuXiaojuan LiuFrontiers Media S.A.articleN-glycosylation modificationN-glycoproteinN-glycanPhaeodactylum tricornutumbiopharmaceuticalsPlant cultureSB1-1110ENFrontiers in Plant Science, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	N-glycosylation modification N-glycoprotein N-glycan Phaeodactylum tricornutum biopharmaceuticals Plant culture SB1-1110
spellingShingle	N-glycosylation modification N-glycoprotein N-glycan Phaeodactylum tricornutum biopharmaceuticals Plant culture SB1-1110 Xihui Xie Xihui Xie Hong Du Hong Du Jichen Chen Jichen Chen Muhammad Aslam Muhammad Aslam Muhammad Aslam Wanna Wang Wanna Wang Weizhou Chen Weizhou Chen Ping Li Hua Du Hua Du Xiaojuan Liu Xiaojuan Liu Global Profiling of N-Glycoproteins and N-Glycans in the Diatom Phaeodactylum tricornutum
description	N-glycosylation is an important posttranslational modification in all eukaryotes, but little is known about the N-glycoproteins and N-glycans in microalgae. Here, N-glycoproteomic and N-glycomic approaches were used to unveil the N-glycoproteins and N-glycans in the model diatom Phaeodactylum tricornutum. In total, 863 different N-glycopeptides corresponding to 639 N-glycoproteins were identified from P. tricornutum. These N-glycoproteins participated in a variety of important metabolic pathways in P. tricornutum. Twelve proteins participating in the N-glycosylation pathway were identified as N-glycoproteins, indicating that the N-glycosylation of these proteins might be important for the protein N-glycosylation pathway. Subsequently, 69 N-glycans corresponding to 59 N-glycoproteins were identified and classified into high mannose and hybrid type N-glycans. High mannose type N-glycans contained four different classes, such as Man-5, Man-7, Man-9, and Man-10 with a terminal glucose residue. Hybrid type N-glycan harbored Man-4 with a terminal GlcNAc residue. The identification of N-glycosylation on nascent proteins expanded our understanding of this modification at a N-glycoproteomic scale, the analysis of N-glycan structures updated the N-glycan database in microalgae. The results obtained from this study facilitate the elucidation of the precise function of these N-glycoproteins and are beneficial for future designing the microalga to produce the functional humanized biopharmaceutical N-glycoproteins for the clinical therapeutics.
format	article
author	Xihui Xie Xihui Xie Hong Du Hong Du Jichen Chen Jichen Chen Muhammad Aslam Muhammad Aslam Muhammad Aslam Wanna Wang Wanna Wang Weizhou Chen Weizhou Chen Ping Li Hua Du Hua Du Xiaojuan Liu Xiaojuan Liu
author_facet	Xihui Xie Xihui Xie Hong Du Hong Du Jichen Chen Jichen Chen Muhammad Aslam Muhammad Aslam Muhammad Aslam Wanna Wang Wanna Wang Weizhou Chen Weizhou Chen Ping Li Hua Du Hua Du Xiaojuan Liu Xiaojuan Liu
author_sort	Xihui Xie
title	Global Profiling of N-Glycoproteins and N-Glycans in the Diatom Phaeodactylum tricornutum
title_short	Global Profiling of N-Glycoproteins and N-Glycans in the Diatom Phaeodactylum tricornutum
title_full	Global Profiling of N-Glycoproteins and N-Glycans in the Diatom Phaeodactylum tricornutum
title_fullStr	Global Profiling of N-Glycoproteins and N-Glycans in the Diatom Phaeodactylum tricornutum
title_full_unstemmed	Global Profiling of N-Glycoproteins and N-Glycans in the Diatom Phaeodactylum tricornutum
title_sort	global profiling of n-glycoproteins and n-glycans in the diatom phaeodactylum tricornutum
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/724e17d247a34a2893d3af0f1385b6fa
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Global Profiling of N-Glycoproteins and N-Glycans in the Diatom Phaeodactylum tricornutum

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