Ankrd31 in Sperm and Epididymal Integrity

Ankyrin proteins (ANKRD) are key mediators linking membrane and sub-membranous cytoskeletal proteins. Recent findings have highlighted a new role of ANKRD31 during spermatogenesis, elucidating its involvement in meiotic recombination and male germ cell progression. Following testicular differentiati...

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Autores principales:	Francesco Manfrevola, Guillaume Martinez, Charles Coutton, Domenico Rocco, Karine Reynaud, Yves Le Vern, Pascal Froment, Linda Beauclair, Denise Aubert, Riccardo Pierantoni, Rosanna Chianese, Florian Guillou
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	ankyrins spermatogenesis sperm quality blood-epididymal-barrier male infertility Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/72516afd023148dc8113f3a40a111e02
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spelling	oai:doaj.org-article:72516afd023148dc8113f3a40a111e022021-11-08T13:35:12ZAnkrd31 in Sperm and Epididymal Integrity2296-634X10.3389/fcell.2021.741975https://doaj.org/article/72516afd023148dc8113f3a40a111e022021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fcell.2021.741975/fullhttps://doaj.org/toc/2296-634XAnkyrin proteins (ANKRD) are key mediators linking membrane and sub-membranous cytoskeletal proteins. Recent findings have highlighted a new role of ANKRD31 during spermatogenesis, elucidating its involvement in meiotic recombination and male germ cell progression. Following testicular differentiation, spermatozoa (SPZ) enter into the epididymis, where they undergo several biochemical and enzymatic changes. The epididymal epithelium is characterized by cell-to-cell junctions that are able to form the blood-epididymal barrier (BEB). This intricate epithelial structure provides the optimal microenvironment needed for epididymal sperm maturation. To date, no notions have been reported regarding a putative role of ANKRD31 in correct BEB formation. In our work, we generated an Ankrd31 knockout male mouse model (Ankrd31–/–) and characterized its reproductive phenotype. Ankrd31–/– mice were infertile and exhibited oligo-astheno-teratozoospermia (a low number of immotile SPZ with abnormal morphological features). In addition, a complete deregulation of BEB was found in Ankrd31–/–, due to cell-to-cell junction anomalies. In order to suggest that BEB deregulation may depend on Ankrd31 gene deletion, we showed the physical interaction among ANKRD31 and some epithelial junction proteins in wild-type (WT) epididymides. In conclusion, the current work shows a key role of ANKRD31 in the control of germ cell progression as well as sperm and epididymal integrity.Francesco ManfrevolaGuillaume MartinezGuillaume MartinezCharles CouttonCharles CouttonDomenico RoccoKarine ReynaudYves Le VernPascal FromentLinda BeauclairDenise AubertRiccardo PierantoniRosanna ChianeseFlorian GuillouFrontiers Media S.A.articleankyrinsspermatogenesissperm qualityblood-epididymal-barriermale infertilityBiology (General)QH301-705.5ENFrontiers in Cell and Developmental Biology, Vol 9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	ankyrins spermatogenesis sperm quality blood-epididymal-barrier male infertility Biology (General) QH301-705.5
spellingShingle	ankyrins spermatogenesis sperm quality blood-epididymal-barrier male infertility Biology (General) QH301-705.5 Francesco Manfrevola Guillaume Martinez Guillaume Martinez Charles Coutton Charles Coutton Domenico Rocco Karine Reynaud Yves Le Vern Pascal Froment Linda Beauclair Denise Aubert Riccardo Pierantoni Rosanna Chianese Florian Guillou Ankrd31 in Sperm and Epididymal Integrity
description	Ankyrin proteins (ANKRD) are key mediators linking membrane and sub-membranous cytoskeletal proteins. Recent findings have highlighted a new role of ANKRD31 during spermatogenesis, elucidating its involvement in meiotic recombination and male germ cell progression. Following testicular differentiation, spermatozoa (SPZ) enter into the epididymis, where they undergo several biochemical and enzymatic changes. The epididymal epithelium is characterized by cell-to-cell junctions that are able to form the blood-epididymal barrier (BEB). This intricate epithelial structure provides the optimal microenvironment needed for epididymal sperm maturation. To date, no notions have been reported regarding a putative role of ANKRD31 in correct BEB formation. In our work, we generated an Ankrd31 knockout male mouse model (Ankrd31–/–) and characterized its reproductive phenotype. Ankrd31–/– mice were infertile and exhibited oligo-astheno-teratozoospermia (a low number of immotile SPZ with abnormal morphological features). In addition, a complete deregulation of BEB was found in Ankrd31–/–, due to cell-to-cell junction anomalies. In order to suggest that BEB deregulation may depend on Ankrd31 gene deletion, we showed the physical interaction among ANKRD31 and some epithelial junction proteins in wild-type (WT) epididymides. In conclusion, the current work shows a key role of ANKRD31 in the control of germ cell progression as well as sperm and epididymal integrity.
format	article
author	Francesco Manfrevola Guillaume Martinez Guillaume Martinez Charles Coutton Charles Coutton Domenico Rocco Karine Reynaud Yves Le Vern Pascal Froment Linda Beauclair Denise Aubert Riccardo Pierantoni Rosanna Chianese Florian Guillou
author_facet	Francesco Manfrevola Guillaume Martinez Guillaume Martinez Charles Coutton Charles Coutton Domenico Rocco Karine Reynaud Yves Le Vern Pascal Froment Linda Beauclair Denise Aubert Riccardo Pierantoni Rosanna Chianese Florian Guillou
author_sort	Francesco Manfrevola
title	Ankrd31 in Sperm and Epididymal Integrity
title_short	Ankrd31 in Sperm and Epididymal Integrity
title_full	Ankrd31 in Sperm and Epididymal Integrity
title_fullStr	Ankrd31 in Sperm and Epididymal Integrity
title_full_unstemmed	Ankrd31 in Sperm and Epididymal Integrity
title_sort	ankrd31 in sperm and epididymal integrity
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/72516afd023148dc8113f3a40a111e02
work_keys_str_mv	AT francescomanfrevola ankrd31inspermandepididymalintegrity AT guillaumemartinez ankrd31inspermandepididymalintegrity AT guillaumemartinez ankrd31inspermandepididymalintegrity AT charlescoutton ankrd31inspermandepididymalintegrity AT charlescoutton ankrd31inspermandepididymalintegrity AT domenicorocco ankrd31inspermandepididymalintegrity AT karinereynaud ankrd31inspermandepididymalintegrity AT yveslevern ankrd31inspermandepididymalintegrity AT pascalfroment ankrd31inspermandepididymalintegrity AT lindabeauclair ankrd31inspermandepididymalintegrity AT deniseaubert ankrd31inspermandepididymalintegrity AT riccardopierantoni ankrd31inspermandepididymalintegrity AT rosannachianese ankrd31inspermandepididymalintegrity AT florianguillou ankrd31inspermandepididymalintegrity
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Ankrd31 in Sperm and Epididymal Integrity

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