Gene expression responses to FUS, EWS, and TAF15 reduction and stress granule sequestration analyses identifies FET-protein non-redundant functions.

The FET family of proteins is composed of FUS/TLS, EWS/EWSR1, and TAF15 and possesses RNA- and DNA-binding capacities. The FET-proteins are involved in transcriptional regulation and RNA processing, and FET-gene deregulation is associated with development of cancer and protein granule formations in...

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Autores principales:	Jenny Blechingberg, Yonglun Luo, Lars Bolund, Christian Kroun Damgaard, Anders Lade Nielsen
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/72584728e6e54bc39eec42b188f4cdb6
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spelling	oai:doaj.org-article:72584728e6e54bc39eec42b188f4cdb62021-11-18T08:14:00ZGene expression responses to FUS, EWS, and TAF15 reduction and stress granule sequestration analyses identifies FET-protein non-redundant functions.1932-620310.1371/journal.pone.0046251https://doaj.org/article/72584728e6e54bc39eec42b188f4cdb62012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23049996/?tool=EBIhttps://doaj.org/toc/1932-6203The FET family of proteins is composed of FUS/TLS, EWS/EWSR1, and TAF15 and possesses RNA- and DNA-binding capacities. The FET-proteins are involved in transcriptional regulation and RNA processing, and FET-gene deregulation is associated with development of cancer and protein granule formations in amyotrophic lateral sclerosis, frontotemporal lobar degeneration, and trinucleotide repeat expansion diseases. We here describe a comparative characterization of FET-protein localization and gene regulatory functions. We show that FUS and TAF15 locate to cellular stress granules to a larger extend than EWS. FET-proteins have no major importance for stress granule formation and cellular stress responses, indicating that FET-protein stress granule association most likely is a downstream response to cellular stress. Gene expression analyses showed that the cellular response towards FUS and TAF15 reduction is relatively similar whereas EWS reduction resulted in a more unique response. The presented data support that FUS and TAF15 are more functionally related to each other, and that the FET-proteins have distinct functions in cellular signaling pathways which could have implications for the neurological disease pathogenesis.Jenny BlechingbergYonglun LuoLars BolundChristian Kroun DamgaardAnders Lade NielsenPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 9, p e46251 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jenny Blechingberg Yonglun Luo Lars Bolund Christian Kroun Damgaard Anders Lade Nielsen Gene expression responses to FUS, EWS, and TAF15 reduction and stress granule sequestration analyses identifies FET-protein non-redundant functions.
description	The FET family of proteins is composed of FUS/TLS, EWS/EWSR1, and TAF15 and possesses RNA- and DNA-binding capacities. The FET-proteins are involved in transcriptional regulation and RNA processing, and FET-gene deregulation is associated with development of cancer and protein granule formations in amyotrophic lateral sclerosis, frontotemporal lobar degeneration, and trinucleotide repeat expansion diseases. We here describe a comparative characterization of FET-protein localization and gene regulatory functions. We show that FUS and TAF15 locate to cellular stress granules to a larger extend than EWS. FET-proteins have no major importance for stress granule formation and cellular stress responses, indicating that FET-protein stress granule association most likely is a downstream response to cellular stress. Gene expression analyses showed that the cellular response towards FUS and TAF15 reduction is relatively similar whereas EWS reduction resulted in a more unique response. The presented data support that FUS and TAF15 are more functionally related to each other, and that the FET-proteins have distinct functions in cellular signaling pathways which could have implications for the neurological disease pathogenesis.
format	article
author	Jenny Blechingberg Yonglun Luo Lars Bolund Christian Kroun Damgaard Anders Lade Nielsen
author_facet	Jenny Blechingberg Yonglun Luo Lars Bolund Christian Kroun Damgaard Anders Lade Nielsen
author_sort	Jenny Blechingberg
title	Gene expression responses to FUS, EWS, and TAF15 reduction and stress granule sequestration analyses identifies FET-protein non-redundant functions.
title_short	Gene expression responses to FUS, EWS, and TAF15 reduction and stress granule sequestration analyses identifies FET-protein non-redundant functions.
title_full	Gene expression responses to FUS, EWS, and TAF15 reduction and stress granule sequestration analyses identifies FET-protein non-redundant functions.
title_fullStr	Gene expression responses to FUS, EWS, and TAF15 reduction and stress granule sequestration analyses identifies FET-protein non-redundant functions.
title_full_unstemmed	Gene expression responses to FUS, EWS, and TAF15 reduction and stress granule sequestration analyses identifies FET-protein non-redundant functions.
title_sort	gene expression responses to fus, ews, and taf15 reduction and stress granule sequestration analyses identifies fet-protein non-redundant functions.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/72584728e6e54bc39eec42b188f4cdb6
work_keys_str_mv	AT jennyblechingberg geneexpressionresponsestofusewsandtaf15reductionandstressgranulesequestrationanalysesidentifiesfetproteinnonredundantfunctions AT yonglunluo geneexpressionresponsestofusewsandtaf15reductionandstressgranulesequestrationanalysesidentifiesfetproteinnonredundantfunctions AT larsbolund geneexpressionresponsestofusewsandtaf15reductionandstressgranulesequestrationanalysesidentifiesfetproteinnonredundantfunctions AT christiankroundamgaard geneexpressionresponsestofusewsandtaf15reductionandstressgranulesequestrationanalysesidentifiesfetproteinnonredundantfunctions AT andersladenielsen geneexpressionresponsestofusewsandtaf15reductionandstressgranulesequestrationanalysesidentifiesfetproteinnonredundantfunctions
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Gene expression responses to FUS, EWS, and TAF15 reduction and stress granule sequestration analyses identifies FET-protein non-redundant functions.

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