Temporal profiling of gene networks associated with the late phase of long-term potentiation in vivo.

Long-term potentiation (LTP) is widely accepted as a cellular mechanism underlying memory processes. It is well established that LTP persistence is strongly dependent on activation of constitutive and inducible transcription factors, but there is limited information regarding the downstream gene net...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Margaret M Ryan, Brigid Ryan, Madeleine Kyrke-Smith, Barbara Logan, Warren P Tate, Wickliffe C Abraham, Joanna M Williams
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7258b114130146e29906e4fa117826a1
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:7258b114130146e29906e4fa117826a1
record_format	dspace
spelling	oai:doaj.org-article:7258b114130146e29906e4fa117826a12021-11-18T07:13:00ZTemporal profiling of gene networks associated with the late phase of long-term potentiation in vivo.1932-620310.1371/journal.pone.0040538https://doaj.org/article/7258b114130146e29906e4fa117826a12012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22802965/?tool=EBIhttps://doaj.org/toc/1932-6203Long-term potentiation (LTP) is widely accepted as a cellular mechanism underlying memory processes. It is well established that LTP persistence is strongly dependent on activation of constitutive and inducible transcription factors, but there is limited information regarding the downstream gene networks and controlling elements that coalesce to stabilise LTP. To identify these gene networks, we used Affymetrix RAT230.2 microarrays to detect genes regulated 5 h and 24 h (n = 5) after LTP induction at perforant path synapses in the dentate gyrus of awake adult rats. The functional relationships of the differentially expressed genes were examined using DAVID and Ingenuity Pathway Analysis, and compared with our previous data derived 20 min post-LTP induction in vivo. This analysis showed that LTP-related genes are predominantly upregulated at 5 h but that there is pronounced downregulation of gene expression at 24 h after LTP induction. Analysis of the structure of the networks and canonical pathways predicted a regulation of calcium dynamics via G-protein coupled receptors, dendritogenesis and neurogenesis at the 5 h time-point. By 24 h neurotrophin-NFKB driven pathways of neuronal growth were identified. The temporal shift in gene expression appears to be mediated by regulation of protein synthesis, ubiquitination and time-dependent regulation of specific microRNA and histone deacetylase expression. Together this programme of genomic responses, marked by both homeostatic and growth pathways, is likely to be critical for the consolidation of LTP in vivo.Margaret M RyanBrigid RyanMadeleine Kyrke-SmithBarbara LoganWarren P TateWickliffe C AbrahamJoanna M WilliamsPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 7, p e40538 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Margaret M Ryan Brigid Ryan Madeleine Kyrke-Smith Barbara Logan Warren P Tate Wickliffe C Abraham Joanna M Williams Temporal profiling of gene networks associated with the late phase of long-term potentiation in vivo.
description	Long-term potentiation (LTP) is widely accepted as a cellular mechanism underlying memory processes. It is well established that LTP persistence is strongly dependent on activation of constitutive and inducible transcription factors, but there is limited information regarding the downstream gene networks and controlling elements that coalesce to stabilise LTP. To identify these gene networks, we used Affymetrix RAT230.2 microarrays to detect genes regulated 5 h and 24 h (n = 5) after LTP induction at perforant path synapses in the dentate gyrus of awake adult rats. The functional relationships of the differentially expressed genes were examined using DAVID and Ingenuity Pathway Analysis, and compared with our previous data derived 20 min post-LTP induction in vivo. This analysis showed that LTP-related genes are predominantly upregulated at 5 h but that there is pronounced downregulation of gene expression at 24 h after LTP induction. Analysis of the structure of the networks and canonical pathways predicted a regulation of calcium dynamics via G-protein coupled receptors, dendritogenesis and neurogenesis at the 5 h time-point. By 24 h neurotrophin-NFKB driven pathways of neuronal growth were identified. The temporal shift in gene expression appears to be mediated by regulation of protein synthesis, ubiquitination and time-dependent regulation of specific microRNA and histone deacetylase expression. Together this programme of genomic responses, marked by both homeostatic and growth pathways, is likely to be critical for the consolidation of LTP in vivo.
format	article
author	Margaret M Ryan Brigid Ryan Madeleine Kyrke-Smith Barbara Logan Warren P Tate Wickliffe C Abraham Joanna M Williams
author_facet	Margaret M Ryan Brigid Ryan Madeleine Kyrke-Smith Barbara Logan Warren P Tate Wickliffe C Abraham Joanna M Williams
author_sort	Margaret M Ryan
title	Temporal profiling of gene networks associated with the late phase of long-term potentiation in vivo.
title_short	Temporal profiling of gene networks associated with the late phase of long-term potentiation in vivo.
title_full	Temporal profiling of gene networks associated with the late phase of long-term potentiation in vivo.
title_fullStr	Temporal profiling of gene networks associated with the late phase of long-term potentiation in vivo.
title_full_unstemmed	Temporal profiling of gene networks associated with the late phase of long-term potentiation in vivo.
title_sort	temporal profiling of gene networks associated with the late phase of long-term potentiation in vivo.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/7258b114130146e29906e4fa117826a1
work_keys_str_mv	AT margaretmryan temporalprofilingofgenenetworksassociatedwiththelatephaseoflongtermpotentiationinvivo AT brigidryan temporalprofilingofgenenetworksassociatedwiththelatephaseoflongtermpotentiationinvivo AT madeleinekyrkesmith temporalprofilingofgenenetworksassociatedwiththelatephaseoflongtermpotentiationinvivo AT barbaralogan temporalprofilingofgenenetworksassociatedwiththelatephaseoflongtermpotentiationinvivo AT warrenptate temporalprofilingofgenenetworksassociatedwiththelatephaseoflongtermpotentiationinvivo AT wickliffecabraham temporalprofilingofgenenetworksassociatedwiththelatephaseoflongtermpotentiationinvivo AT joannamwilliams temporalprofilingofgenenetworksassociatedwiththelatephaseoflongtermpotentiationinvivo
_version_	1718423795324157952

Temporal profiling of gene networks associated with the late phase of long-term potentiation in vivo.

Ejemplares similares