pERK-mediated IL8 secretion can enhance the migration, invasion, and cisplatin resistance of CD10-positive oral cancer cells

Abstract Background Cancer stem cells (CSCs) drive tumor initiation and progression and participate in tumor chemoresistance. We recently discovered that oral squamous cell carcinoma (OSCC) cells that highly express CD10 (CD10H cells) present cancer stem cells (CSC)-associated characteristics, which...

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Autores principales:	Yinfei Pu, Qingxiang Li, Yifei Wang, Le Xu, Qiao Qiao, Yuxing Guo, Chuanbin Guo
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	IL8, CD10, p-ERK Oral squamous cell carcinoma Drug resistance Cisplatin Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
Acceso en línea:	https://doaj.org/article/72645804ed624fdfadd04be79ad13845
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spelling	oai:doaj.org-article:72645804ed624fdfadd04be79ad138452021-12-05T12:19:39ZpERK-mediated IL8 secretion can enhance the migration, invasion, and cisplatin resistance of CD10-positive oral cancer cells10.1186/s12885-021-09025-71471-2407https://doaj.org/article/72645804ed624fdfadd04be79ad138452021-12-01T00:00:00Zhttps://doi.org/10.1186/s12885-021-09025-7https://doaj.org/toc/1471-2407Abstract Background Cancer stem cells (CSCs) drive tumor initiation and progression and participate in tumor chemoresistance. We recently discovered that oral squamous cell carcinoma (OSCC) cells that highly express CD10 (CD10H cells) present cancer stem cells (CSC)-associated characteristics, which, in turn, affect the tumor growth, epithelial-mesenchymal transition (EMT), and resistance to cisplatin. In this study, we further investigated this mechanism in vitro and in vivo. We hypothesized that IL8 might regulate migration, invasion, and cisplatin resistance of CD10-positive oral cancer cells through the ERK pathway. Methods CD10 MicroBead Kit was used to select HN6 cells with high and low expression of CD10. The target protein IL8 was screened via protein chip assay. Lentiviral transduction and specific inhibitor were applied to investigate the signaling pathway. Real-time PCR, Western blot, and immunohistochemistry were used to analyze the mRNA and protein expression; transwell assay, spheroid formation assay, and cell viability assay were used to study the cell biological behavior in vitro; xenograft animal model was used to evaluate the tumor formation rate in vivo. Results Overexpression of CD10 promoted CSC-related genes expression and enhanced migration, invasion, spheroid formation, and chemoresistance in HN6 cells. Moreover, the overexpression of IL8 was detected in OSCC tumor tissue and cell lines (HN6 and CAL27) overexpressing CD10. IL8 secreted by CD10H HN6 promoted migration and invasion and restored tumor chemosensitivity via the p-ERK signaling pathway, while the inhibition of IL8 increased the chemosensitivity to cisplatin. Conclusions IL8 secretion by CD10 positive cells promotes migration, invasion, and cisplatin resistance of OSCC via the p-ERK signaling pathway.Yinfei PuQingxiang LiYifei WangLe XuQiao QiaoYuxing GuoChuanbin GuoBMCarticleIL8, CD10, p-ERKOral squamous cell carcinomaDrug resistanceCisplatinNeoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282ENBMC Cancer, Vol 21, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	IL8, CD10, p-ERK Oral squamous cell carcinoma Drug resistance Cisplatin Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
spellingShingle	IL8, CD10, p-ERK Oral squamous cell carcinoma Drug resistance Cisplatin Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Yinfei Pu Qingxiang Li Yifei Wang Le Xu Qiao Qiao Yuxing Guo Chuanbin Guo pERK-mediated IL8 secretion can enhance the migration, invasion, and cisplatin resistance of CD10-positive oral cancer cells
description	Abstract Background Cancer stem cells (CSCs) drive tumor initiation and progression and participate in tumor chemoresistance. We recently discovered that oral squamous cell carcinoma (OSCC) cells that highly express CD10 (CD10H cells) present cancer stem cells (CSC)-associated characteristics, which, in turn, affect the tumor growth, epithelial-mesenchymal transition (EMT), and resistance to cisplatin. In this study, we further investigated this mechanism in vitro and in vivo. We hypothesized that IL8 might regulate migration, invasion, and cisplatin resistance of CD10-positive oral cancer cells through the ERK pathway. Methods CD10 MicroBead Kit was used to select HN6 cells with high and low expression of CD10. The target protein IL8 was screened via protein chip assay. Lentiviral transduction and specific inhibitor were applied to investigate the signaling pathway. Real-time PCR, Western blot, and immunohistochemistry were used to analyze the mRNA and protein expression; transwell assay, spheroid formation assay, and cell viability assay were used to study the cell biological behavior in vitro; xenograft animal model was used to evaluate the tumor formation rate in vivo. Results Overexpression of CD10 promoted CSC-related genes expression and enhanced migration, invasion, spheroid formation, and chemoresistance in HN6 cells. Moreover, the overexpression of IL8 was detected in OSCC tumor tissue and cell lines (HN6 and CAL27) overexpressing CD10. IL8 secreted by CD10H HN6 promoted migration and invasion and restored tumor chemosensitivity via the p-ERK signaling pathway, while the inhibition of IL8 increased the chemosensitivity to cisplatin. Conclusions IL8 secretion by CD10 positive cells promotes migration, invasion, and cisplatin resistance of OSCC via the p-ERK signaling pathway.
format	article
author	Yinfei Pu Qingxiang Li Yifei Wang Le Xu Qiao Qiao Yuxing Guo Chuanbin Guo
author_facet	Yinfei Pu Qingxiang Li Yifei Wang Le Xu Qiao Qiao Yuxing Guo Chuanbin Guo
author_sort	Yinfei Pu
title	pERK-mediated IL8 secretion can enhance the migration, invasion, and cisplatin resistance of CD10-positive oral cancer cells
title_short	pERK-mediated IL8 secretion can enhance the migration, invasion, and cisplatin resistance of CD10-positive oral cancer cells
title_full	pERK-mediated IL8 secretion can enhance the migration, invasion, and cisplatin resistance of CD10-positive oral cancer cells
title_fullStr	pERK-mediated IL8 secretion can enhance the migration, invasion, and cisplatin resistance of CD10-positive oral cancer cells
title_full_unstemmed	pERK-mediated IL8 secretion can enhance the migration, invasion, and cisplatin resistance of CD10-positive oral cancer cells
title_sort	perk-mediated il8 secretion can enhance the migration, invasion, and cisplatin resistance of cd10-positive oral cancer cells
publisher	BMC
publishDate	2021
url	https://doaj.org/article/72645804ed624fdfadd04be79ad13845
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_version_	1718372023645765632

pERK-mediated IL8 secretion can enhance the migration, invasion, and cisplatin resistance of CD10-positive oral cancer cells

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