The effect of red-allotrope selenium nanoparticles on head and neck squamous cell viability and growth

Christopher E Hassan,1 Thomas J Webster1,2 1Department of Chemical Engineering, Northeastern University, Boston, MA, USA; 2Center of Excellence for Advanced Materials Research, King Abdulaziz University, Jeddah, Saudi Arabia Abstract: Given their low toxicity and natural presence in the h...

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Autores principales:	Hassan CE, Webster TJ
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2016
Materias:	Selenium nanotechnology biomaterials head and neck squamous cell carcinoma human dermal fibroblasts Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/726a79d40076468dbb83d851303d54df
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spelling	oai:doaj.org-article:726a79d40076468dbb83d851303d54df2021-12-02T07:46:15ZThe effect of red-allotrope selenium nanoparticles on head and neck squamous cell viability and growth1178-2013https://doaj.org/article/726a79d40076468dbb83d851303d54df2016-08-01T00:00:00Zhttps://www.dovepress.com/the-effect-of-red-allotrope-selenium-nanoparticles-on-head-and-neck-sq-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Christopher E Hassan,1 Thomas J Webster1,2 1Department of Chemical Engineering, Northeastern University, Boston, MA, USA; 2Center of Excellence for Advanced Materials Research, King Abdulaziz University, Jeddah, Saudi Arabia Abstract: Given their low toxicity and natural presence in the human diet, selenium nanoparticles have been established as potential candidates for the treatment of numerous cancers. Red-allotrope selenium nanoparticles (rSeNPs) were synthesized and characterized in this study. Head and neck squamous cell carcinoma (HNSCC) and human dermal fibroblast (HDF) cells were cultured and exposed to rSeNPs at concentrations ranging from 0.01 to 100 µg rSeNP/mL media for 1–3 days. The toxicity of rSeNP toward HNSCC and HDFs was analyzed. Results indicated that the particles were approximately four times as cytotoxic toward HNSCC compared to HDFs, with their respective IC50 values at 19.22 and 59.61 µg rSeNP/mL media. Using statistical analysis, an effective dosage range for killing HNSCC cells while simultaneously minimizing damage to HDFs over a 3-day incubation period was established at 20–55 µg rSeNP/mL media. Observations showed that doses of rSeNP <5 µg rSeNP/mL media resulted in cell proliferation. Transmission electron microscopy images of HNSCC and HDF cells, both treated with rSeNPs, revealed that the rSeNPs became localized in the cytoplasm near the lysosomes and mitochondria. Analysis of cell morphology showed that the rSeNPs primarily induced HNSCC apoptosis. Collectively, these results indicated that rSeNPs are a promising option for treating HNSCC without adversely affecting healthy cells and without resorting to the use of harmful chemotherapeutics. Keywords: selenium, nanotechnology, biomaterials, head and neck squamous cell carcinoma, human dermal fibroblasts, cancer Hassan CEWebster TJDove Medical PressarticleSeleniumnanotechnologybiomaterialshead and neck squamous cell carcinomahuman dermal fibroblastsMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2016, Iss default, Pp 3641-3654 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	Selenium nanotechnology biomaterials head and neck squamous cell carcinoma human dermal fibroblasts Medicine (General) R5-920
spellingShingle	Selenium nanotechnology biomaterials head and neck squamous cell carcinoma human dermal fibroblasts Medicine (General) R5-920 Hassan CE Webster TJ The effect of red-allotrope selenium nanoparticles on head and neck squamous cell viability and growth
description	Christopher E Hassan,1 Thomas J Webster1,2 1Department of Chemical Engineering, Northeastern University, Boston, MA, USA; 2Center of Excellence for Advanced Materials Research, King Abdulaziz University, Jeddah, Saudi Arabia Abstract: Given their low toxicity and natural presence in the human diet, selenium nanoparticles have been established as potential candidates for the treatment of numerous cancers. Red-allotrope selenium nanoparticles (rSeNPs) were synthesized and characterized in this study. Head and neck squamous cell carcinoma (HNSCC) and human dermal fibroblast (HDF) cells were cultured and exposed to rSeNPs at concentrations ranging from 0.01 to 100 µg rSeNP/mL media for 1–3 days. The toxicity of rSeNP toward HNSCC and HDFs was analyzed. Results indicated that the particles were approximately four times as cytotoxic toward HNSCC compared to HDFs, with their respective IC50 values at 19.22 and 59.61 µg rSeNP/mL media. Using statistical analysis, an effective dosage range for killing HNSCC cells while simultaneously minimizing damage to HDFs over a 3-day incubation period was established at 20–55 µg rSeNP/mL media. Observations showed that doses of rSeNP <5 µg rSeNP/mL media resulted in cell proliferation. Transmission electron microscopy images of HNSCC and HDF cells, both treated with rSeNPs, revealed that the rSeNPs became localized in the cytoplasm near the lysosomes and mitochondria. Analysis of cell morphology showed that the rSeNPs primarily induced HNSCC apoptosis. Collectively, these results indicated that rSeNPs are a promising option for treating HNSCC without adversely affecting healthy cells and without resorting to the use of harmful chemotherapeutics. Keywords: selenium, nanotechnology, biomaterials, head and neck squamous cell carcinoma, human dermal fibroblasts, cancer
format	article
author	Hassan CE Webster TJ
author_facet	Hassan CE Webster TJ
author_sort	Hassan CE
title	The effect of red-allotrope selenium nanoparticles on head and neck squamous cell viability and growth
title_short	The effect of red-allotrope selenium nanoparticles on head and neck squamous cell viability and growth
title_full	The effect of red-allotrope selenium nanoparticles on head and neck squamous cell viability and growth
title_fullStr	The effect of red-allotrope selenium nanoparticles on head and neck squamous cell viability and growth
title_full_unstemmed	The effect of red-allotrope selenium nanoparticles on head and neck squamous cell viability and growth
title_sort	effect of red-allotrope selenium nanoparticles on head and neck squamous cell viability and growth
publisher	Dove Medical Press
publishDate	2016
url	https://doaj.org/article/726a79d40076468dbb83d851303d54df
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The effect of red-allotrope selenium nanoparticles on head and neck squamous cell viability and growth

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