Tailoring seed oil composition in the real world: optimising omega-3 long chain polyunsaturated fatty acid accumulation in transgenic Camelina sativa

Abstract There is considerable interest in the de novo production of omega-3 long chain polyunsaturated fatty acids such as eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), not least of all given the importance of these fatty acids in both aquaculture and human nutrition. Previously we ha...

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Autores principales:	Sarah Usher, Lihua Han, Richard P. Haslam, Louise V. Michaelson, Drew Sturtevant, Mina Aziz, Kent D. Chapman, Olga Sayanova, Johnathan A. Napier
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/726cb36a0f524cb19397a52c385dfd09
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spelling	oai:doaj.org-article:726cb36a0f524cb19397a52c385dfd092021-12-02T15:05:25ZTailoring seed oil composition in the real world: optimising omega-3 long chain polyunsaturated fatty acid accumulation in transgenic Camelina sativa10.1038/s41598-017-06838-02045-2322https://doaj.org/article/726cb36a0f524cb19397a52c385dfd092017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06838-0https://doaj.org/toc/2045-2322Abstract There is considerable interest in the de novo production of omega-3 long chain polyunsaturated fatty acids such as eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), not least of all given the importance of these fatty acids in both aquaculture and human nutrition. Previously we have demonstrated the feasibility of using metabolic engineering in transgenic plants (Camelina sativa) to modify the seed oil composition to now include EPA and/or DHA. In this study, we further tailored the seed oil profile to reduce the omega-6 content, and evaluated the performance of such GM plants under field conditions (i.e. environmental releases), in terms of agronomic performance and also the lipidomic profile of seed oil. We used MALDI- mass spectrometry imaging to identify discrete tissue-types in the seed in which these non-native fatty acids preferentially accumulated. Collectively, these data provide new insights into the complexity of plant lipid metabolism and the challenges associated with predictive manipulation of these pathways. However, this study identified the likely dispensable nature of a Δ12-desturase activity in our omega-3 metabolic engineering rationales for Camelina.Sarah UsherLihua HanRichard P. HaslamLouise V. MichaelsonDrew SturtevantMina AzizKent D. ChapmanOlga SayanovaJohnathan A. NapierNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sarah Usher Lihua Han Richard P. Haslam Louise V. Michaelson Drew Sturtevant Mina Aziz Kent D. Chapman Olga Sayanova Johnathan A. Napier Tailoring seed oil composition in the real world: optimising omega-3 long chain polyunsaturated fatty acid accumulation in transgenic Camelina sativa
description	Abstract There is considerable interest in the de novo production of omega-3 long chain polyunsaturated fatty acids such as eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), not least of all given the importance of these fatty acids in both aquaculture and human nutrition. Previously we have demonstrated the feasibility of using metabolic engineering in transgenic plants (Camelina sativa) to modify the seed oil composition to now include EPA and/or DHA. In this study, we further tailored the seed oil profile to reduce the omega-6 content, and evaluated the performance of such GM plants under field conditions (i.e. environmental releases), in terms of agronomic performance and also the lipidomic profile of seed oil. We used MALDI- mass spectrometry imaging to identify discrete tissue-types in the seed in which these non-native fatty acids preferentially accumulated. Collectively, these data provide new insights into the complexity of plant lipid metabolism and the challenges associated with predictive manipulation of these pathways. However, this study identified the likely dispensable nature of a Δ12-desturase activity in our omega-3 metabolic engineering rationales for Camelina.
format	article
author	Sarah Usher Lihua Han Richard P. Haslam Louise V. Michaelson Drew Sturtevant Mina Aziz Kent D. Chapman Olga Sayanova Johnathan A. Napier
author_facet	Sarah Usher Lihua Han Richard P. Haslam Louise V. Michaelson Drew Sturtevant Mina Aziz Kent D. Chapman Olga Sayanova Johnathan A. Napier
author_sort	Sarah Usher
title	Tailoring seed oil composition in the real world: optimising omega-3 long chain polyunsaturated fatty acid accumulation in transgenic Camelina sativa
title_short	Tailoring seed oil composition in the real world: optimising omega-3 long chain polyunsaturated fatty acid accumulation in transgenic Camelina sativa
title_full	Tailoring seed oil composition in the real world: optimising omega-3 long chain polyunsaturated fatty acid accumulation in transgenic Camelina sativa
title_fullStr	Tailoring seed oil composition in the real world: optimising omega-3 long chain polyunsaturated fatty acid accumulation in transgenic Camelina sativa
title_full_unstemmed	Tailoring seed oil composition in the real world: optimising omega-3 long chain polyunsaturated fatty acid accumulation in transgenic Camelina sativa
title_sort	tailoring seed oil composition in the real world: optimising omega-3 long chain polyunsaturated fatty acid accumulation in transgenic camelina sativa
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/726cb36a0f524cb19397a52c385dfd09
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Tailoring seed oil composition in the real world: optimising omega-3 long chain polyunsaturated fatty acid accumulation in transgenic Camelina sativa

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