A pre-screening strategy to assess resected tumor margins by imaging cytoplasmic viscosity and hypoxia
To assure complete tumor removal, frozen section analysis is the most common procedure for intraoperative pathological assessment of resected tumor margins. However, during one operation, multiple biopsies may be sent for examination, but only few of them are made into cryosections because of the co...
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eLife Sciences Publications Ltd
2021
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oai:doaj.org-article:726d1170cc6b4532a6f30685452d07c12021-12-01T11:04:16ZA pre-screening strategy to assess resected tumor margins by imaging cytoplasmic viscosity and hypoxia10.7554/eLife.704712050-084Xe70471https://doaj.org/article/726d1170cc6b4532a6f30685452d07c12021-10-01T00:00:00Zhttps://elifesciences.org/articles/70471https://doaj.org/toc/2050-084XTo assure complete tumor removal, frozen section analysis is the most common procedure for intraoperative pathological assessment of resected tumor margins. However, during one operation, multiple biopsies may be sent for examination, but only few of them are made into cryosections because of the complex preparation protocols and time-consuming pathological analysis, which potentially increases the risk of overlooking tumor involvement. Here, we propose a fluorescence-based pre-screening strategy that allows high-throughput, convenient, and fast gross assessment of resected tumor margins. A dual-activatable cationic fluorescent molecular rotor was developed to specifically illuminate live tumor cells’ cytoplasm by emitting two different fluorescence signals in response to elevations in hypoxia-induced nitroreductase (a biochemical marker) and cytoplasmic viscosity (a biophysical marker), two characteristics of cancer cells. The ability of the fluorescent molecular rotor in detecting tumor cells was evaluated in mouse and human specimens of multiple tissues by comparing with hematoxylin and eosin staining. Importantly, the fluorescent molecular rotor achieved 100 % specificity in discriminating lung and liver cancers from normal tissue, allowing pre-screening of the tumor-free surgical margins and promoting clinical decision. Altogether, this type of fluorescent molecular rotor and the proposed strategy may serve as a new option to facilitate intraoperative assessment of resected tumor margins.Hui HuangYoupei LinWenrui MaJiannan LiuJing HanXiaoyi HuMeilin TangShiqiang YanMieradilijiang AbudupataerChenping ZhangQiang GaoWeijia ZhangeLife Sciences Publications Ltdarticlepre-screening strategyfluorescent molecular rotordual-activatablecytoplasmic viscosityhypoxiaMedicineRScienceQBiology (General)QH301-705.5ENeLife, Vol 10 (2021) |
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DOAJ |
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pre-screening strategy fluorescent molecular rotor dual-activatable cytoplasmic viscosity hypoxia Medicine R Science Q Biology (General) QH301-705.5 |
| spellingShingle |
pre-screening strategy fluorescent molecular rotor dual-activatable cytoplasmic viscosity hypoxia Medicine R Science Q Biology (General) QH301-705.5 Hui Huang Youpei Lin Wenrui Ma Jiannan Liu Jing Han Xiaoyi Hu Meilin Tang Shiqiang Yan Mieradilijiang Abudupataer Chenping Zhang Qiang Gao Weijia Zhang A pre-screening strategy to assess resected tumor margins by imaging cytoplasmic viscosity and hypoxia |
| description |
To assure complete tumor removal, frozen section analysis is the most common procedure for intraoperative pathological assessment of resected tumor margins. However, during one operation, multiple biopsies may be sent for examination, but only few of them are made into cryosections because of the complex preparation protocols and time-consuming pathological analysis, which potentially increases the risk of overlooking tumor involvement. Here, we propose a fluorescence-based pre-screening strategy that allows high-throughput, convenient, and fast gross assessment of resected tumor margins. A dual-activatable cationic fluorescent molecular rotor was developed to specifically illuminate live tumor cells’ cytoplasm by emitting two different fluorescence signals in response to elevations in hypoxia-induced nitroreductase (a biochemical marker) and cytoplasmic viscosity (a biophysical marker), two characteristics of cancer cells. The ability of the fluorescent molecular rotor in detecting tumor cells was evaluated in mouse and human specimens of multiple tissues by comparing with hematoxylin and eosin staining. Importantly, the fluorescent molecular rotor achieved 100 % specificity in discriminating lung and liver cancers from normal tissue, allowing pre-screening of the tumor-free surgical margins and promoting clinical decision. Altogether, this type of fluorescent molecular rotor and the proposed strategy may serve as a new option to facilitate intraoperative assessment of resected tumor margins. |
| format |
article |
| author |
Hui Huang Youpei Lin Wenrui Ma Jiannan Liu Jing Han Xiaoyi Hu Meilin Tang Shiqiang Yan Mieradilijiang Abudupataer Chenping Zhang Qiang Gao Weijia Zhang |
| author_facet |
Hui Huang Youpei Lin Wenrui Ma Jiannan Liu Jing Han Xiaoyi Hu Meilin Tang Shiqiang Yan Mieradilijiang Abudupataer Chenping Zhang Qiang Gao Weijia Zhang |
| author_sort |
Hui Huang |
| title |
A pre-screening strategy to assess resected tumor margins by imaging cytoplasmic viscosity and hypoxia |
| title_short |
A pre-screening strategy to assess resected tumor margins by imaging cytoplasmic viscosity and hypoxia |
| title_full |
A pre-screening strategy to assess resected tumor margins by imaging cytoplasmic viscosity and hypoxia |
| title_fullStr |
A pre-screening strategy to assess resected tumor margins by imaging cytoplasmic viscosity and hypoxia |
| title_full_unstemmed |
A pre-screening strategy to assess resected tumor margins by imaging cytoplasmic viscosity and hypoxia |
| title_sort |
pre-screening strategy to assess resected tumor margins by imaging cytoplasmic viscosity and hypoxia |
| publisher |
eLife Sciences Publications Ltd |
| publishDate |
2021 |
| url |
https://doaj.org/article/726d1170cc6b4532a6f30685452d07c1 |
| work_keys_str_mv |
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| _version_ |
1718405239762059264 |