Bioinformatic Analyses and Experimental Verification Reveal that High FSTL3 Expression Promotes EMT via Fibronectin-1/α5β1 Interaction in Colorectal Cancer
Background: Colorectal cancer (CRC) is a typical cancer prevalent worldwide. Despite the conventional treatments, CRC has a poor prognosis due to relapse and metastasis. Moreover, there is a dearth of sensitive biomarkers for predicting prognosis in CRC.Methods: This study used a bioinformatics appr...
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Frontiers Media S.A.
2021
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oai:doaj.org-article:7270a790ad75429fa3a28be4539955892021-11-30T18:51:06ZBioinformatic Analyses and Experimental Verification Reveal that High FSTL3 Expression Promotes EMT via Fibronectin-1/α5β1 Interaction in Colorectal Cancer2296-889X10.3389/fmolb.2021.762924https://doaj.org/article/7270a790ad75429fa3a28be4539955892021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmolb.2021.762924/fullhttps://doaj.org/toc/2296-889XBackground: Colorectal cancer (CRC) is a typical cancer prevalent worldwide. Despite the conventional treatments, CRC has a poor prognosis due to relapse and metastasis. Moreover, there is a dearth of sensitive biomarkers for predicting prognosis in CRC.Methods: This study used a bioinformatics approach combining validation experiments to examine the value of follistatin-like 3 (FSTL3) as a prognostic predictor and therapeutic target in CRC.Results:FSTL3 was remarkably upregulated in the CRC samples. FSTL3 overexpression was significantly associated with a poor prognosis. FSTL3 was found to activate the epithelial-mesenchymal transition by promoting the binding of FN1 to α5β1. FSTL3 expression was also positively correlated with the abundance of the potent immunosuppressors, M2 macrophages.Conclusion:FSTL3 overexpression affects CRC prognosis and thus, FSTL3 can be a prognostic biomarker and therapeutic target with potential applications in CRC.Yuanjie LiuYuanjie LiuJiepin LiJiepin LiJiepin LiShuhong ZengShuhong ZengYing ZhangYing ZhangYonghua ZhangZhichao JinShenlin LiuShenlin LiuXi ZouFrontiers Media S.A.articlecolorecal cancerFSTL3 geneEMT—epithelial to mesenchymal transformationFN1fibronectin 1α5β1 integrinBiology (General)QH301-705.5ENFrontiers in Molecular Biosciences, Vol 8 (2021) |
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colorecal cancer FSTL3 gene EMT—epithelial to mesenchymal transformation FN1 fibronectin 1 α5β1 integrin Biology (General) QH301-705.5 |
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colorecal cancer FSTL3 gene EMT—epithelial to mesenchymal transformation FN1 fibronectin 1 α5β1 integrin Biology (General) QH301-705.5 Yuanjie Liu Yuanjie Liu Jiepin Li Jiepin Li Jiepin Li Shuhong Zeng Shuhong Zeng Ying Zhang Ying Zhang Yonghua Zhang Zhichao Jin Shenlin Liu Shenlin Liu Xi Zou Bioinformatic Analyses and Experimental Verification Reveal that High FSTL3 Expression Promotes EMT via Fibronectin-1/α5β1 Interaction in Colorectal Cancer |
| description |
Background: Colorectal cancer (CRC) is a typical cancer prevalent worldwide. Despite the conventional treatments, CRC has a poor prognosis due to relapse and metastasis. Moreover, there is a dearth of sensitive biomarkers for predicting prognosis in CRC.Methods: This study used a bioinformatics approach combining validation experiments to examine the value of follistatin-like 3 (FSTL3) as a prognostic predictor and therapeutic target in CRC.Results:FSTL3 was remarkably upregulated in the CRC samples. FSTL3 overexpression was significantly associated with a poor prognosis. FSTL3 was found to activate the epithelial-mesenchymal transition by promoting the binding of FN1 to α5β1. FSTL3 expression was also positively correlated with the abundance of the potent immunosuppressors, M2 macrophages.Conclusion:FSTL3 overexpression affects CRC prognosis and thus, FSTL3 can be a prognostic biomarker and therapeutic target with potential applications in CRC. |
| format |
article |
| author |
Yuanjie Liu Yuanjie Liu Jiepin Li Jiepin Li Jiepin Li Shuhong Zeng Shuhong Zeng Ying Zhang Ying Zhang Yonghua Zhang Zhichao Jin Shenlin Liu Shenlin Liu Xi Zou |
| author_facet |
Yuanjie Liu Yuanjie Liu Jiepin Li Jiepin Li Jiepin Li Shuhong Zeng Shuhong Zeng Ying Zhang Ying Zhang Yonghua Zhang Zhichao Jin Shenlin Liu Shenlin Liu Xi Zou |
| author_sort |
Yuanjie Liu |
| title |
Bioinformatic Analyses and Experimental Verification Reveal that High FSTL3 Expression Promotes EMT via Fibronectin-1/α5β1 Interaction in Colorectal Cancer |
| title_short |
Bioinformatic Analyses and Experimental Verification Reveal that High FSTL3 Expression Promotes EMT via Fibronectin-1/α5β1 Interaction in Colorectal Cancer |
| title_full |
Bioinformatic Analyses and Experimental Verification Reveal that High FSTL3 Expression Promotes EMT via Fibronectin-1/α5β1 Interaction in Colorectal Cancer |
| title_fullStr |
Bioinformatic Analyses and Experimental Verification Reveal that High FSTL3 Expression Promotes EMT via Fibronectin-1/α5β1 Interaction in Colorectal Cancer |
| title_full_unstemmed |
Bioinformatic Analyses and Experimental Verification Reveal that High FSTL3 Expression Promotes EMT via Fibronectin-1/α5β1 Interaction in Colorectal Cancer |
| title_sort |
bioinformatic analyses and experimental verification reveal that high fstl3 expression promotes emt via fibronectin-1/α5β1 interaction in colorectal cancer |
| publisher |
Frontiers Media S.A. |
| publishDate |
2021 |
| url |
https://doaj.org/article/7270a790ad75429fa3a28be453995589 |
| work_keys_str_mv |
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