661W is a retinal ganglion precursor-like cell line in which glaucoma-associated optineurin mutants induce cell death selectively

Abstract A photoreceptor cell line, 661W, derived from a mouse retinal tumor that expresses several markers of cone photoreceptor cells has been described earlier. However, these cells can be differentiated into neuronal cells. Here, we report that this cell line expressed certain markers specific t...

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Autores principales:	Zuberwasim Sayyad, Kapil Sirohi, Vegesna Radha, Ghanshyam Swarup
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/72730e92429a43929b5a51022c8d1c75
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spelling	oai:doaj.org-article:72730e92429a43929b5a51022c8d1c752021-12-02T15:04:58Z661W is a retinal ganglion precursor-like cell line in which glaucoma-associated optineurin mutants induce cell death selectively10.1038/s41598-017-17241-02045-2322https://doaj.org/article/72730e92429a43929b5a51022c8d1c752017-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-17241-0https://doaj.org/toc/2045-2322Abstract A photoreceptor cell line, 661W, derived from a mouse retinal tumor that expresses several markers of cone photoreceptor cells has been described earlier. However, these cells can be differentiated into neuronal cells. Here, we report that this cell line expressed certain markers specific to retinal ganglion cells such as Rbpms, Brn3b (Pou4f2), Brn3c (Pou4f3), Thy1 and γ-synuclein (Sncg), and some other markers of neuronal cells (beta-III tubulin, NeuN and MAP2). These cells also expressed Opn1mw, a cone-specific marker and nestin, a marker for neural precursor cells. Two glaucoma-associated mutants of OPTN, E50K and M98K, but not an amyotrophic lateral sclerosis-associated mutant, E478G, induced cell death selectively in 661W cells. However, in a motor neuron cell line, NSC34, E478G mutant of OPTN but not E50K and M98K induced cell death. We conclude that 661W is a retinal ganglion precursor-like cell line, which shows properties of both retinal ganglion and photoreceptor cells. We suggest that these cells could be utilized for exploring the mechanisms of cell death induction and cytoprotection relevant for glaucoma pathogenesis. RGC-5 cell line which probably arose from 661W cells showed expression of essentially the same markers of retinal ganglion cells and neuronal cells as seen in 661W cells.Zuberwasim SayyadKapil SirohiVegesna RadhaGhanshyam SwarupNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Zuberwasim Sayyad Kapil Sirohi Vegesna Radha Ghanshyam Swarup 661W is a retinal ganglion precursor-like cell line in which glaucoma-associated optineurin mutants induce cell death selectively
description	Abstract A photoreceptor cell line, 661W, derived from a mouse retinal tumor that expresses several markers of cone photoreceptor cells has been described earlier. However, these cells can be differentiated into neuronal cells. Here, we report that this cell line expressed certain markers specific to retinal ganglion cells such as Rbpms, Brn3b (Pou4f2), Brn3c (Pou4f3), Thy1 and γ-synuclein (Sncg), and some other markers of neuronal cells (beta-III tubulin, NeuN and MAP2). These cells also expressed Opn1mw, a cone-specific marker and nestin, a marker for neural precursor cells. Two glaucoma-associated mutants of OPTN, E50K and M98K, but not an amyotrophic lateral sclerosis-associated mutant, E478G, induced cell death selectively in 661W cells. However, in a motor neuron cell line, NSC34, E478G mutant of OPTN but not E50K and M98K induced cell death. We conclude that 661W is a retinal ganglion precursor-like cell line, which shows properties of both retinal ganglion and photoreceptor cells. We suggest that these cells could be utilized for exploring the mechanisms of cell death induction and cytoprotection relevant for glaucoma pathogenesis. RGC-5 cell line which probably arose from 661W cells showed expression of essentially the same markers of retinal ganglion cells and neuronal cells as seen in 661W cells.
format	article
author	Zuberwasim Sayyad Kapil Sirohi Vegesna Radha Ghanshyam Swarup
author_facet	Zuberwasim Sayyad Kapil Sirohi Vegesna Radha Ghanshyam Swarup
author_sort	Zuberwasim Sayyad
title	661W is a retinal ganglion precursor-like cell line in which glaucoma-associated optineurin mutants induce cell death selectively
title_short	661W is a retinal ganglion precursor-like cell line in which glaucoma-associated optineurin mutants induce cell death selectively
title_full	661W is a retinal ganglion precursor-like cell line in which glaucoma-associated optineurin mutants induce cell death selectively
title_fullStr	661W is a retinal ganglion precursor-like cell line in which glaucoma-associated optineurin mutants induce cell death selectively
title_full_unstemmed	661W is a retinal ganglion precursor-like cell line in which glaucoma-associated optineurin mutants induce cell death selectively
title_sort	661w is a retinal ganglion precursor-like cell line in which glaucoma-associated optineurin mutants induce cell death selectively
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/72730e92429a43929b5a51022c8d1c75
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661W is a retinal ganglion precursor-like cell line in which glaucoma-associated optineurin mutants induce cell death selectively

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