Genotypic diversity and drug susceptibility patterns among M. tuberculosis complex isolates from South-Western Ghana.

<h4>Objective</h4>The aim of this study was to use spoligotyping and large sequence polymorphism (LSP) to study the population structure of M. tuberculosis complex (MTBC) isolates.<h4>Methods</h4>MTBC isolates were identified using standard biochemical procedures, IS6110 PCR,...

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Autores principales:	Dorothy Yeboah-Manu, Adwoa Asante-Poku, Thomas Bodmer, David Stucki, Kwadwo Koram, Frank Bonsu, Gerd Pluschke, Sebastien Gagneux
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/727324ebd1e44f6784e3cbfa84567a9c
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spelling	oai:doaj.org-article:727324ebd1e44f6784e3cbfa84567a9c2021-11-18T06:50:28ZGenotypic diversity and drug susceptibility patterns among M. tuberculosis complex isolates from South-Western Ghana.1932-620310.1371/journal.pone.0021906https://doaj.org/article/727324ebd1e44f6784e3cbfa84567a9c2011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21779354/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Objective</h4>The aim of this study was to use spoligotyping and large sequence polymorphism (LSP) to study the population structure of M. tuberculosis complex (MTBC) isolates.<h4>Methods</h4>MTBC isolates were identified using standard biochemical procedures, IS6110 PCR, and large sequence polymorphisms. Isolates were further typed using spoligotyping, and the phenotypic drug susceptibility patterns were determined by the proportion method.<h4>Result</h4>One hundred and sixty-two isolates were characterised by LSP typing. Of these, 130 (80.25%) were identified as Mycobacterium tuberculosis sensu stricto (MTBss), with the Cameroon sub-lineage being dominant (N = 59/130, 45.38%). Thirty-two (19.75%) isolates were classified as Mycobacterium africanum type 1, and of these 26 (81.25%) were identified as West-Africa I, and 6 (18.75%) as West-Africa II. Spoligotyping sub-lineages identified among the MTBss included Haarlem (N = 15, 11.53%), Ghana (N = 22, 16.92%), Beijing (4, 3.08%), EAI (4, 3.08%), Uganda I (4, 3.08%), LAM (2, 1.54%), X (N = 1, 0.77%) and S (2, 1.54%). Nine isolates had SIT numbers with no identified sub-lineages while 17 had no SIT numbers. MTBss isolates were more likely to be resistant to streptomycin (p<0.008) and to any drug resistance (p<0.03) when compared to M. africanum.<h4>Conclusion</h4>This study demonstrated that overall 36.4% of TB in South-Western Ghana is caused by the Cameroon sub-lineage of MTBC and 20% by M. africanum type 1, including both the West-Africa 1 and West-Africa 2 lineages. The diversity of MTBC in Ghana should be considered when evaluating new TB vaccines.Dorothy Yeboah-ManuAdwoa Asante-PokuThomas BodmerDavid StuckiKwadwo KoramFrank BonsuGerd PluschkeSebastien GagneuxPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 7, p e21906 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Dorothy Yeboah-Manu Adwoa Asante-Poku Thomas Bodmer David Stucki Kwadwo Koram Frank Bonsu Gerd Pluschke Sebastien Gagneux Genotypic diversity and drug susceptibility patterns among M. tuberculosis complex isolates from South-Western Ghana.
description	<h4>Objective</h4>The aim of this study was to use spoligotyping and large sequence polymorphism (LSP) to study the population structure of M. tuberculosis complex (MTBC) isolates.<h4>Methods</h4>MTBC isolates were identified using standard biochemical procedures, IS6110 PCR, and large sequence polymorphisms. Isolates were further typed using spoligotyping, and the phenotypic drug susceptibility patterns were determined by the proportion method.<h4>Result</h4>One hundred and sixty-two isolates were characterised by LSP typing. Of these, 130 (80.25%) were identified as Mycobacterium tuberculosis sensu stricto (MTBss), with the Cameroon sub-lineage being dominant (N = 59/130, 45.38%). Thirty-two (19.75%) isolates were classified as Mycobacterium africanum type 1, and of these 26 (81.25%) were identified as West-Africa I, and 6 (18.75%) as West-Africa II. Spoligotyping sub-lineages identified among the MTBss included Haarlem (N = 15, 11.53%), Ghana (N = 22, 16.92%), Beijing (4, 3.08%), EAI (4, 3.08%), Uganda I (4, 3.08%), LAM (2, 1.54%), X (N = 1, 0.77%) and S (2, 1.54%). Nine isolates had SIT numbers with no identified sub-lineages while 17 had no SIT numbers. MTBss isolates were more likely to be resistant to streptomycin (p<0.008) and to any drug resistance (p<0.03) when compared to M. africanum.<h4>Conclusion</h4>This study demonstrated that overall 36.4% of TB in South-Western Ghana is caused by the Cameroon sub-lineage of MTBC and 20% by M. africanum type 1, including both the West-Africa 1 and West-Africa 2 lineages. The diversity of MTBC in Ghana should be considered when evaluating new TB vaccines.
format	article
author	Dorothy Yeboah-Manu Adwoa Asante-Poku Thomas Bodmer David Stucki Kwadwo Koram Frank Bonsu Gerd Pluschke Sebastien Gagneux
author_facet	Dorothy Yeboah-Manu Adwoa Asante-Poku Thomas Bodmer David Stucki Kwadwo Koram Frank Bonsu Gerd Pluschke Sebastien Gagneux
author_sort	Dorothy Yeboah-Manu
title	Genotypic diversity and drug susceptibility patterns among M. tuberculosis complex isolates from South-Western Ghana.
title_short	Genotypic diversity and drug susceptibility patterns among M. tuberculosis complex isolates from South-Western Ghana.
title_full	Genotypic diversity and drug susceptibility patterns among M. tuberculosis complex isolates from South-Western Ghana.
title_fullStr	Genotypic diversity and drug susceptibility patterns among M. tuberculosis complex isolates from South-Western Ghana.
title_full_unstemmed	Genotypic diversity and drug susceptibility patterns among M. tuberculosis complex isolates from South-Western Ghana.
title_sort	genotypic diversity and drug susceptibility patterns among m. tuberculosis complex isolates from south-western ghana.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/727324ebd1e44f6784e3cbfa84567a9c
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Genotypic diversity and drug susceptibility patterns among M. tuberculosis complex isolates from South-Western Ghana.

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