Generation of LUMCi041-A-2: Equipping a PAX3 reporter iPSC line with doxycycline inducible H2B-mTurquoise2 for live cell imaging

An induced pluripotent stem cell (iPSC) line, in which a H2B-fluorescent protein fusion is temporally expressed, is a valuable tool to track cells and study cell divisions and apoptosis. To this end we introduced a 3rd generation “all-in-one” doxycycline-inducible H2B-mTurquoise2 vector into the AAV...

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Autores principales:	C.H. Arendzen, U. Chaudhari, S.J. Cramer, C.M.A.H. Freund, C.L. Mummery, A. Ranga, O. Pourquie, H.M.M. Mikkers
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	hiPS cell line Cell-Cylce Reporter Histon2B Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/7275645b52964a4e9595823d143f6969
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spelling	oai:doaj.org-article:7275645b52964a4e9595823d143f69692021-11-14T04:31:24ZGeneration of LUMCi041-A-2: Equipping a PAX3 reporter iPSC line with doxycycline inducible H2B-mTurquoise2 for live cell imaging1873-506110.1016/j.scr.2021.102592https://doaj.org/article/7275645b52964a4e9595823d143f69692021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S1873506121004396https://doaj.org/toc/1873-5061An induced pluripotent stem cell (iPSC) line, in which a H2B-fluorescent protein fusion is temporally expressed, is a valuable tool to track cells and study cell divisions and apoptosis. To this end we introduced a 3rd generation “all-in-one” doxycycline-inducible H2B-mTurquoise2 vector into the AAVS1 locus of PAX3-Venus iPSCs via CRISPR/Cas9. H2B-mTurquoise2 expression is absent but readily induced by doxycycline allowing quantification of cell divisions and imaging of living cells. Besides being a universal reporter in iPSC-based differentiation and toxicity assays, the generated pluripotent and genomically normal LUMCi041-A-2 line is particularly suited to study PAX3-positive stages of development.C.H. ArendzenU. ChaudhariS.J. CramerC.M.A.H. FreundC.L. MummeryA. RangaO. PourquieH.M.M. MikkersElsevierarticlehiPS cell lineCell-Cylce ReporterHiston2BBiology (General)QH301-705.5ENStem Cell Research, Vol 57, Iss , Pp 102592- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	hiPS cell line Cell-Cylce Reporter Histon2B Biology (General) QH301-705.5
spellingShingle	hiPS cell line Cell-Cylce Reporter Histon2B Biology (General) QH301-705.5 C.H. Arendzen U. Chaudhari S.J. Cramer C.M.A.H. Freund C.L. Mummery A. Ranga O. Pourquie H.M.M. Mikkers Generation of LUMCi041-A-2: Equipping a PAX3 reporter iPSC line with doxycycline inducible H2B-mTurquoise2 for live cell imaging
description	An induced pluripotent stem cell (iPSC) line, in which a H2B-fluorescent protein fusion is temporally expressed, is a valuable tool to track cells and study cell divisions and apoptosis. To this end we introduced a 3rd generation “all-in-one” doxycycline-inducible H2B-mTurquoise2 vector into the AAVS1 locus of PAX3-Venus iPSCs via CRISPR/Cas9. H2B-mTurquoise2 expression is absent but readily induced by doxycycline allowing quantification of cell divisions and imaging of living cells. Besides being a universal reporter in iPSC-based differentiation and toxicity assays, the generated pluripotent and genomically normal LUMCi041-A-2 line is particularly suited to study PAX3-positive stages of development.
format	article
author	C.H. Arendzen U. Chaudhari S.J. Cramer C.M.A.H. Freund C.L. Mummery A. Ranga O. Pourquie H.M.M. Mikkers
author_facet	C.H. Arendzen U. Chaudhari S.J. Cramer C.M.A.H. Freund C.L. Mummery A. Ranga O. Pourquie H.M.M. Mikkers
author_sort	C.H. Arendzen
title	Generation of LUMCi041-A-2: Equipping a PAX3 reporter iPSC line with doxycycline inducible H2B-mTurquoise2 for live cell imaging
title_short	Generation of LUMCi041-A-2: Equipping a PAX3 reporter iPSC line with doxycycline inducible H2B-mTurquoise2 for live cell imaging
title_full	Generation of LUMCi041-A-2: Equipping a PAX3 reporter iPSC line with doxycycline inducible H2B-mTurquoise2 for live cell imaging
title_fullStr	Generation of LUMCi041-A-2: Equipping a PAX3 reporter iPSC line with doxycycline inducible H2B-mTurquoise2 for live cell imaging
title_full_unstemmed	Generation of LUMCi041-A-2: Equipping a PAX3 reporter iPSC line with doxycycline inducible H2B-mTurquoise2 for live cell imaging
title_sort	generation of lumci041-a-2: equipping a pax3 reporter ipsc line with doxycycline inducible h2b-mturquoise2 for live cell imaging
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/7275645b52964a4e9595823d143f6969
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Generation of LUMCi041-A-2: Equipping a PAX3 reporter iPSC line with doxycycline inducible H2B-mTurquoise2 for live cell imaging

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