Upregulation of circulating microRNA-134 in adult-onset Still’s disease and its use as potential biomarker

Abstract Adult-onset Still’s disease (AOSD) is a multi-systemic inflammatory disorder of unknown etiology. To date, no single diagnostic test is available for AOSD. Herein, we investigated the pathogenic role of microRNAs in AOSD. MicroRNA profiles in plasma from AOSD patients and healthy controls w...

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Autores principales:	Tsai-Ling Liao, Yi-Ming Chen, Chia-Wei Hsieh, Hsin-Hua Chen, Hsiu-Chin Lee, Wei-Ting Hung, Kuo-Tung Tang, Der-Yuan Chen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7276b5b8e7c34af1b6ca83815c1c01a5
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spelling	oai:doaj.org-article:7276b5b8e7c34af1b6ca83815c1c01a52021-12-02T16:08:07ZUpregulation of circulating microRNA-134 in adult-onset Still’s disease and its use as potential biomarker10.1038/s41598-017-04086-w2045-2322https://doaj.org/article/7276b5b8e7c34af1b6ca83815c1c01a52017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04086-whttps://doaj.org/toc/2045-2322Abstract Adult-onset Still’s disease (AOSD) is a multi-systemic inflammatory disorder of unknown etiology. To date, no single diagnostic test is available for AOSD. Herein, we investigated the pathogenic role of microRNAs in AOSD. MicroRNA profiles in plasma from AOSD patients and healthy controls were analyzed by microarray analysis, followed by quantitative reverse transcription PCR validation. The biological functions of microRNAs were evaluated using in vitro cell-based assay. Among the differentially expressed microRNAs, microRNA-134 (miR-134) expression was positively correlated with AOSD activity scores and significantly decreased after effective treatment. An increased miR-134 level is significantly associated with the activation of Toll-like receptor 3 (TLR3). The reporter assay identified IL-18 binding protein (IL-18BP) as the target of miR-134. A negative correlation between miR-134 expression and IL-18BP mRNA levels were detected in peripheral blood cells following TLR3 ligand treatment. Lower plasma IL-18BP levels and higher IL-18 levels were also observed in active AOSD patients who had higher miR-134 expression than inactive patients. Upregulation of circulating miR-134 was associated with elevated IL-18 levels by targeting IL-18BP in AOSD patients and was positively correlated with disease activity, suggesting its involvement in AOSD pathogenesis. MiR-134 may be a novel activity indicator or potential prognostic biomarker in AOSD.Tsai-Ling LiaoYi-Ming ChenChia-Wei HsiehHsin-Hua ChenHsiu-Chin LeeWei-Ting HungKuo-Tung TangDer-Yuan ChenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Tsai-Ling Liao Yi-Ming Chen Chia-Wei Hsieh Hsin-Hua Chen Hsiu-Chin Lee Wei-Ting Hung Kuo-Tung Tang Der-Yuan Chen Upregulation of circulating microRNA-134 in adult-onset Still’s disease and its use as potential biomarker
description	Abstract Adult-onset Still’s disease (AOSD) is a multi-systemic inflammatory disorder of unknown etiology. To date, no single diagnostic test is available for AOSD. Herein, we investigated the pathogenic role of microRNAs in AOSD. MicroRNA profiles in plasma from AOSD patients and healthy controls were analyzed by microarray analysis, followed by quantitative reverse transcription PCR validation. The biological functions of microRNAs were evaluated using in vitro cell-based assay. Among the differentially expressed microRNAs, microRNA-134 (miR-134) expression was positively correlated with AOSD activity scores and significantly decreased after effective treatment. An increased miR-134 level is significantly associated with the activation of Toll-like receptor 3 (TLR3). The reporter assay identified IL-18 binding protein (IL-18BP) as the target of miR-134. A negative correlation between miR-134 expression and IL-18BP mRNA levels were detected in peripheral blood cells following TLR3 ligand treatment. Lower plasma IL-18BP levels and higher IL-18 levels were also observed in active AOSD patients who had higher miR-134 expression than inactive patients. Upregulation of circulating miR-134 was associated with elevated IL-18 levels by targeting IL-18BP in AOSD patients and was positively correlated with disease activity, suggesting its involvement in AOSD pathogenesis. MiR-134 may be a novel activity indicator or potential prognostic biomarker in AOSD.
format	article
author	Tsai-Ling Liao Yi-Ming Chen Chia-Wei Hsieh Hsin-Hua Chen Hsiu-Chin Lee Wei-Ting Hung Kuo-Tung Tang Der-Yuan Chen
author_facet	Tsai-Ling Liao Yi-Ming Chen Chia-Wei Hsieh Hsin-Hua Chen Hsiu-Chin Lee Wei-Ting Hung Kuo-Tung Tang Der-Yuan Chen
author_sort	Tsai-Ling Liao
title	Upregulation of circulating microRNA-134 in adult-onset Still’s disease and its use as potential biomarker
title_short	Upregulation of circulating microRNA-134 in adult-onset Still’s disease and its use as potential biomarker
title_full	Upregulation of circulating microRNA-134 in adult-onset Still’s disease and its use as potential biomarker
title_fullStr	Upregulation of circulating microRNA-134 in adult-onset Still’s disease and its use as potential biomarker
title_full_unstemmed	Upregulation of circulating microRNA-134 in adult-onset Still’s disease and its use as potential biomarker
title_sort	upregulation of circulating microrna-134 in adult-onset still’s disease and its use as potential biomarker
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/7276b5b8e7c34af1b6ca83815c1c01a5
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Upregulation of circulating microRNA-134 in adult-onset Still’s disease and its use as potential biomarker

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