The RNA-binding protein MARF1 promotes cortical neurogenesis through its RNase activity domain

Abstract Cortical neurogenesis is a fundamental process of brain development that is spatiotemporally regulated by both intrinsic and extrinsic cues. Although recent evidence has highlighted the significance of transcription factors in cortical neurogenesis, little is known regarding the role of RNA...

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Autores principales:	Yoshitaka Kanemitsu, Masashi Fujitani, Yuki Fujita, Suxiang Zhang, You-Qiang Su, Yukio Kawahara, Toshihide Yamashita
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/72811c53c1224cf6a19e1357979e9cc7
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spelling	oai:doaj.org-article:72811c53c1224cf6a19e1357979e9cc72021-12-02T11:52:33ZThe RNA-binding protein MARF1 promotes cortical neurogenesis through its RNase activity domain10.1038/s41598-017-01317-y2045-2322https://doaj.org/article/72811c53c1224cf6a19e1357979e9cc72017-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01317-yhttps://doaj.org/toc/2045-2322Abstract Cortical neurogenesis is a fundamental process of brain development that is spatiotemporally regulated by both intrinsic and extrinsic cues. Although recent evidence has highlighted the significance of transcription factors in cortical neurogenesis, little is known regarding the role of RNA-binding proteins (RBPs) in the post-transcriptional regulation of cortical neurogenesis. Here, we report that meiosis arrest female 1 (MARF1) is an RBP that is expressed during neuronal differentiation. Cortical neurons expressed the somatic form of MARF1 (sMARF1) but not the oocyte form (oMARF1). sMARF1 was enriched in embryonic brains, and its expression level decreased as brain development progressed. Overexpression of sMARF1 in E12.5 neuronal progenitor cells promoted neuronal differentiation, whereas sMARF1 knockdown decreased neuronal progenitor differentiation in vitro. We also examined the function of sMARF1 in vivo using an in utero electroporation technique. Overexpression of sMARF1 increased neuronal differentiation, whereas knockdown of sMARF1 inhibited differentiation in vivo. Moreover, using an RNase domain deletion mutant of sMARF1, we showed that the RNase domain is required for the effects of sMARF1 on cortical neurogenesis in vitro. Our results further elucidate the mechanisms of post-transcriptional regulation of cortical neurogenesis by RBPs.Yoshitaka KanemitsuMasashi FujitaniYuki FujitaSuxiang ZhangYou-Qiang SuYukio KawaharaToshihide YamashitaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yoshitaka Kanemitsu Masashi Fujitani Yuki Fujita Suxiang Zhang You-Qiang Su Yukio Kawahara Toshihide Yamashita The RNA-binding protein MARF1 promotes cortical neurogenesis through its RNase activity domain
description	Abstract Cortical neurogenesis is a fundamental process of brain development that is spatiotemporally regulated by both intrinsic and extrinsic cues. Although recent evidence has highlighted the significance of transcription factors in cortical neurogenesis, little is known regarding the role of RNA-binding proteins (RBPs) in the post-transcriptional regulation of cortical neurogenesis. Here, we report that meiosis arrest female 1 (MARF1) is an RBP that is expressed during neuronal differentiation. Cortical neurons expressed the somatic form of MARF1 (sMARF1) but not the oocyte form (oMARF1). sMARF1 was enriched in embryonic brains, and its expression level decreased as brain development progressed. Overexpression of sMARF1 in E12.5 neuronal progenitor cells promoted neuronal differentiation, whereas sMARF1 knockdown decreased neuronal progenitor differentiation in vitro. We also examined the function of sMARF1 in vivo using an in utero electroporation technique. Overexpression of sMARF1 increased neuronal differentiation, whereas knockdown of sMARF1 inhibited differentiation in vivo. Moreover, using an RNase domain deletion mutant of sMARF1, we showed that the RNase domain is required for the effects of sMARF1 on cortical neurogenesis in vitro. Our results further elucidate the mechanisms of post-transcriptional regulation of cortical neurogenesis by RBPs.
format	article
author	Yoshitaka Kanemitsu Masashi Fujitani Yuki Fujita Suxiang Zhang You-Qiang Su Yukio Kawahara Toshihide Yamashita
author_facet	Yoshitaka Kanemitsu Masashi Fujitani Yuki Fujita Suxiang Zhang You-Qiang Su Yukio Kawahara Toshihide Yamashita
author_sort	Yoshitaka Kanemitsu
title	The RNA-binding protein MARF1 promotes cortical neurogenesis through its RNase activity domain
title_short	The RNA-binding protein MARF1 promotes cortical neurogenesis through its RNase activity domain
title_full	The RNA-binding protein MARF1 promotes cortical neurogenesis through its RNase activity domain
title_fullStr	The RNA-binding protein MARF1 promotes cortical neurogenesis through its RNase activity domain
title_full_unstemmed	The RNA-binding protein MARF1 promotes cortical neurogenesis through its RNase activity domain
title_sort	rna-binding protein marf1 promotes cortical neurogenesis through its rnase activity domain
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/72811c53c1224cf6a19e1357979e9cc7
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The RNA-binding protein MARF1 promotes cortical neurogenesis through its RNase activity domain

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