Analysis of β-tubulin-carbendazim interaction reveals that binding site for MBC fungicides does not include residues involved in fungicide resistance
Abstract Methyl benzimidazole carbamate (MBC) fungicides are fungicidal compounds that exert their biological activities by preventing cell division through the inhibition of tubulin polymerization, which is the major component of microtubules. Several mutations in the β-tubulin gene contribute to M...
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2018
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oai:doaj.org-article:729189f47e3149a6a20735532b1500dc2021-12-02T11:40:25ZAnalysis of β-tubulin-carbendazim interaction reveals that binding site for MBC fungicides does not include residues involved in fungicide resistance10.1038/s41598-018-25336-52045-2322https://doaj.org/article/729189f47e3149a6a20735532b1500dc2018-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-25336-5https://doaj.org/toc/2045-2322Abstract Methyl benzimidazole carbamate (MBC) fungicides are fungicidal compounds that exert their biological activities by preventing cell division through the inhibition of tubulin polymerization, which is the major component of microtubules. Several mutations in the β-tubulin gene contribute to MBC resistance, the most common and significant of which occur at residues 198 and 200. Despite nearly 50 years of agricultural use, the binding site of MBCs and the precise mechanism by which those mutations affect fungicide efficacy have not been determined. The aim of this work was to clarify the mode of action and the mechanism of resistance to MBC fungicides in Podosphaera xanthii, the primary causal agent of cucurbit powdery mildew, using a combination of biochemical, biophysical and computational approaches. The results allow us to propose an MBC binding site in β-tubulin that lies close to the GTP binding site and does not include residue 198 involved in MBC resistance.David Vela-CorcíaDiego RomeroAntonio de VicenteAlejandro Pérez-GarcíaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-12 (2018) |
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Medicine R Science Q David Vela-Corcía Diego Romero Antonio de Vicente Alejandro Pérez-García Analysis of β-tubulin-carbendazim interaction reveals that binding site for MBC fungicides does not include residues involved in fungicide resistance |
description |
Abstract Methyl benzimidazole carbamate (MBC) fungicides are fungicidal compounds that exert their biological activities by preventing cell division through the inhibition of tubulin polymerization, which is the major component of microtubules. Several mutations in the β-tubulin gene contribute to MBC resistance, the most common and significant of which occur at residues 198 and 200. Despite nearly 50 years of agricultural use, the binding site of MBCs and the precise mechanism by which those mutations affect fungicide efficacy have not been determined. The aim of this work was to clarify the mode of action and the mechanism of resistance to MBC fungicides in Podosphaera xanthii, the primary causal agent of cucurbit powdery mildew, using a combination of biochemical, biophysical and computational approaches. The results allow us to propose an MBC binding site in β-tubulin that lies close to the GTP binding site and does not include residue 198 involved in MBC resistance. |
format |
article |
author |
David Vela-Corcía Diego Romero Antonio de Vicente Alejandro Pérez-García |
author_facet |
David Vela-Corcía Diego Romero Antonio de Vicente Alejandro Pérez-García |
author_sort |
David Vela-Corcía |
title |
Analysis of β-tubulin-carbendazim interaction reveals that binding site for MBC fungicides does not include residues involved in fungicide resistance |
title_short |
Analysis of β-tubulin-carbendazim interaction reveals that binding site for MBC fungicides does not include residues involved in fungicide resistance |
title_full |
Analysis of β-tubulin-carbendazim interaction reveals that binding site for MBC fungicides does not include residues involved in fungicide resistance |
title_fullStr |
Analysis of β-tubulin-carbendazim interaction reveals that binding site for MBC fungicides does not include residues involved in fungicide resistance |
title_full_unstemmed |
Analysis of β-tubulin-carbendazim interaction reveals that binding site for MBC fungicides does not include residues involved in fungicide resistance |
title_sort |
analysis of β-tubulin-carbendazim interaction reveals that binding site for mbc fungicides does not include residues involved in fungicide resistance |
publisher |
Nature Portfolio |
publishDate |
2018 |
url |
https://doaj.org/article/729189f47e3149a6a20735532b1500dc |
work_keys_str_mv |
AT davidvelacorcia analysisofbtubulincarbendaziminteractionrevealsthatbindingsiteformbcfungicidesdoesnotincluderesiduesinvolvedinfungicideresistance AT diegoromero analysisofbtubulincarbendaziminteractionrevealsthatbindingsiteformbcfungicidesdoesnotincluderesiduesinvolvedinfungicideresistance AT antoniodevicente analysisofbtubulincarbendaziminteractionrevealsthatbindingsiteformbcfungicidesdoesnotincluderesiduesinvolvedinfungicideresistance AT alejandroperezgarcia analysisofbtubulincarbendaziminteractionrevealsthatbindingsiteformbcfungicidesdoesnotincluderesiduesinvolvedinfungicideresistance |
_version_ |
1718395650688679936 |