Measurement of nanoscale three-dimensional diffusion in the interior of living cells by STED-FCS

The measurement of molecular diffusion at sub-diffraction scales has been achieved in 2D space using STED-FCS, but an implementation for 3D diffusion is lacking. Here the authors present an analytical approach to probe diffusion in 3D space using STED-FCS and measure the diffusion of EGFP at differe...

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Autores principales:	Luca Lanzanò, Lorenzo Scipioni, Melody Di Bona, Paolo Bianchini, Ranieri Bizzarri, Francesco Cardarelli, Alberto Diaspro, Giuseppe Vicidomini
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/7291b1d354344449ac27eaf8ba4af319
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spelling	oai:doaj.org-article:7291b1d354344449ac27eaf8ba4af3192021-12-02T13:57:44ZMeasurement of nanoscale three-dimensional diffusion in the interior of living cells by STED-FCS10.1038/s41467-017-00117-22041-1723https://doaj.org/article/7291b1d354344449ac27eaf8ba4af3192017-07-01T00:00:00Zhttps://doi.org/10.1038/s41467-017-00117-2https://doaj.org/toc/2041-1723The measurement of molecular diffusion at sub-diffraction scales has been achieved in 2D space using STED-FCS, but an implementation for 3D diffusion is lacking. Here the authors present an analytical approach to probe diffusion in 3D space using STED-FCS and measure the diffusion of EGFP at different spatial scales.Luca LanzanòLorenzo ScipioniMelody Di BonaPaolo BianchiniRanieri BizzarriFrancesco CardarelliAlberto DiasproGiuseppe VicidominiNature PortfolioarticleScienceQENNature Communications, Vol 8, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Luca Lanzanò Lorenzo Scipioni Melody Di Bona Paolo Bianchini Ranieri Bizzarri Francesco Cardarelli Alberto Diaspro Giuseppe Vicidomini Measurement of nanoscale three-dimensional diffusion in the interior of living cells by STED-FCS
description	The measurement of molecular diffusion at sub-diffraction scales has been achieved in 2D space using STED-FCS, but an implementation for 3D diffusion is lacking. Here the authors present an analytical approach to probe diffusion in 3D space using STED-FCS and measure the diffusion of EGFP at different spatial scales.
format	article
author	Luca Lanzanò Lorenzo Scipioni Melody Di Bona Paolo Bianchini Ranieri Bizzarri Francesco Cardarelli Alberto Diaspro Giuseppe Vicidomini
author_facet	Luca Lanzanò Lorenzo Scipioni Melody Di Bona Paolo Bianchini Ranieri Bizzarri Francesco Cardarelli Alberto Diaspro Giuseppe Vicidomini
author_sort	Luca Lanzanò
title	Measurement of nanoscale three-dimensional diffusion in the interior of living cells by STED-FCS
title_short	Measurement of nanoscale three-dimensional diffusion in the interior of living cells by STED-FCS
title_full	Measurement of nanoscale three-dimensional diffusion in the interior of living cells by STED-FCS
title_fullStr	Measurement of nanoscale three-dimensional diffusion in the interior of living cells by STED-FCS
title_full_unstemmed	Measurement of nanoscale three-dimensional diffusion in the interior of living cells by STED-FCS
title_sort	measurement of nanoscale three-dimensional diffusion in the interior of living cells by sted-fcs
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/7291b1d354344449ac27eaf8ba4af319
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Measurement of nanoscale three-dimensional diffusion in the interior of living cells by STED-FCS

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