Measurement of nanoscale three-dimensional diffusion in the interior of living cells by STED-FCS

The measurement of molecular diffusion at sub-diffraction scales has been achieved in 2D space using STED-FCS, but an implementation for 3D diffusion is lacking. Here the authors present an analytical approach to probe diffusion in 3D space using STED-FCS and measure the diffusion of EGFP at differe...

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Autores principales: Luca Lanzanò, Lorenzo Scipioni, Melody Di Bona, Paolo Bianchini, Ranieri Bizzarri, Francesco Cardarelli, Alberto Diaspro, Giuseppe Vicidomini
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2017
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Acceso en línea:https://doaj.org/article/7291b1d354344449ac27eaf8ba4af319
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