1,2,3, MHC: a review of mass-spectrometry-based immunopeptidomics methods for relative and absolute quantification of pMHCs

Quantitative mass-spectrometry-based methods to perform relative and absolute quantification of peptides in the immunopeptidome are growing in popularity as researchers aim to measure the dynamic nature of the peptide major histocompatibility complex repertoire and make copies-per-cell estimations o...

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Autores principales:	L.E. Stopfer, A.D. D'Souza, F.M. White
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	HLA MHC immunopeptidomics mass spectometry antigen presentation Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
Acceso en línea:	https://doaj.org/article/729f4a8f0c1c4d338b61f399cbe28506
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spelling	oai:doaj.org-article:729f4a8f0c1c4d338b61f399cbe285062021-12-04T04:35:39Z1,2,3, MHC: a review of mass-spectrometry-based immunopeptidomics methods for relative and absolute quantification of pMHCs2590-018810.1016/j.iotech.2021.100042https://doaj.org/article/729f4a8f0c1c4d338b61f399cbe285062021-10-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2590018821000174https://doaj.org/toc/2590-0188Quantitative mass-spectrometry-based methods to perform relative and absolute quantification of peptides in the immunopeptidome are growing in popularity as researchers aim to measure the dynamic nature of the peptide major histocompatibility complex repertoire and make copies-per-cell estimations of target antigens of interest. Multiple methods to carry out these experiments have been reported, each with unique advantages and limitations. This article describes existing methods and recent applications, offering guidance for improving quantitative accuracy and selecting an appropriate experimental set-up to maximize data quality and quantity.L.E. StopferA.D. D'SouzaF.M. WhiteElsevierarticleHLAMHCimmunopeptidomicsmass spectometryantigen presentationNeoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282ENImmuno-Oncology and Technology, Vol 11, Iss , Pp 100042- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	HLA MHC immunopeptidomics mass spectometry antigen presentation Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
spellingShingle	HLA MHC immunopeptidomics mass spectometry antigen presentation Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 L.E. Stopfer A.D. D'Souza F.M. White 1,2,3, MHC: a review of mass-spectrometry-based immunopeptidomics methods for relative and absolute quantification of pMHCs
description	Quantitative mass-spectrometry-based methods to perform relative and absolute quantification of peptides in the immunopeptidome are growing in popularity as researchers aim to measure the dynamic nature of the peptide major histocompatibility complex repertoire and make copies-per-cell estimations of target antigens of interest. Multiple methods to carry out these experiments have been reported, each with unique advantages and limitations. This article describes existing methods and recent applications, offering guidance for improving quantitative accuracy and selecting an appropriate experimental set-up to maximize data quality and quantity.
format	article
author	L.E. Stopfer A.D. D'Souza F.M. White
author_facet	L.E. Stopfer A.D. D'Souza F.M. White
author_sort	L.E. Stopfer
title	1,2,3, MHC: a review of mass-spectrometry-based immunopeptidomics methods for relative and absolute quantification of pMHCs
title_short	1,2,3, MHC: a review of mass-spectrometry-based immunopeptidomics methods for relative and absolute quantification of pMHCs
title_full	1,2,3, MHC: a review of mass-spectrometry-based immunopeptidomics methods for relative and absolute quantification of pMHCs
title_fullStr	1,2,3, MHC: a review of mass-spectrometry-based immunopeptidomics methods for relative and absolute quantification of pMHCs
title_full_unstemmed	1,2,3, MHC: a review of mass-spectrometry-based immunopeptidomics methods for relative and absolute quantification of pMHCs
title_sort	1,2,3, mhc: a review of mass-spectrometry-based immunopeptidomics methods for relative and absolute quantification of pmhcs
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/729f4a8f0c1c4d338b61f399cbe28506
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1,2,3, MHC: a review of mass-spectrometry-based immunopeptidomics methods for relative and absolute quantification of pMHCs

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