The RNA binding protein Csx1 promotes sexual differentiation in Schizosaccharomyces pombe.

Sexual differentiation is a highly regulated process in the fission yeast Schizosaccharomyces pombe and is triggered by nutrient depletion, mainly nitrogen source. One of the key regulatory proteins in fission yeast sexual differentiation is the transcription factor Ste11. Ste11 regulates the transc...

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Autores principales:	Ana M Matia-Gonzalez, Jael Sotelo, Miguel A Rodriguez-Gabriel
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/72ad1c3219a24fc28504b0348b55d364
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spelling	oai:doaj.org-article:72ad1c3219a24fc28504b0348b55d3642021-11-18T07:30:26ZThe RNA binding protein Csx1 promotes sexual differentiation in Schizosaccharomyces pombe.1932-620310.1371/journal.pone.0030067https://doaj.org/article/72ad1c3219a24fc28504b0348b55d3642012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22253882/?tool=EBIhttps://doaj.org/toc/1932-6203Sexual differentiation is a highly regulated process in the fission yeast Schizosaccharomyces pombe and is triggered by nutrient depletion, mainly nitrogen source. One of the key regulatory proteins in fission yeast sexual differentiation is the transcription factor Ste11. Ste11 regulates the transcription of many genes required for the initial steps of conjugation and meiosis, and its deficiency leads to sterility. Ste11 activity is mainly regulated at two levels: phosphorylation and abundance of its mRNA. Csx1 is an RNA binding protein that we have previously described to bind and regulate the turnover rate of the mRNA encoding the transcription factor Atf1 in the presence of oxidative stress. We have observed that Csx1-deficient cells have defects in sexual differentiation and are partially sterile. We investigated how Csx1 is regulating this process in S. pombe. Csx1 associates with ste11+ mRNA and cells lacking Csx1 are sterile with a reduced amount of ste11+ mRNA. Overexpression of ste11+ mRNA completely rescues the mating deficiencies of csx1Δ cells. Here, we present a novel mechanism of ste11+ mRNA positive regulation through the activity of Csx1, an RNA binding protein that also have key functions in the response to oxidative stress in fission yeast. This finding opens interesting question about the possible coordination of sexual differentiation and oxidative stress response in eukaryotes and the role of RNA binding proteins in the adaptation to environmental signals.Ana M Matia-GonzalezJael SoteloMiguel A Rodriguez-GabrielPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 1, p e30067 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ana M Matia-Gonzalez Jael Sotelo Miguel A Rodriguez-Gabriel The RNA binding protein Csx1 promotes sexual differentiation in Schizosaccharomyces pombe.
description	Sexual differentiation is a highly regulated process in the fission yeast Schizosaccharomyces pombe and is triggered by nutrient depletion, mainly nitrogen source. One of the key regulatory proteins in fission yeast sexual differentiation is the transcription factor Ste11. Ste11 regulates the transcription of many genes required for the initial steps of conjugation and meiosis, and its deficiency leads to sterility. Ste11 activity is mainly regulated at two levels: phosphorylation and abundance of its mRNA. Csx1 is an RNA binding protein that we have previously described to bind and regulate the turnover rate of the mRNA encoding the transcription factor Atf1 in the presence of oxidative stress. We have observed that Csx1-deficient cells have defects in sexual differentiation and are partially sterile. We investigated how Csx1 is regulating this process in S. pombe. Csx1 associates with ste11+ mRNA and cells lacking Csx1 are sterile with a reduced amount of ste11+ mRNA. Overexpression of ste11+ mRNA completely rescues the mating deficiencies of csx1Δ cells. Here, we present a novel mechanism of ste11+ mRNA positive regulation through the activity of Csx1, an RNA binding protein that also have key functions in the response to oxidative stress in fission yeast. This finding opens interesting question about the possible coordination of sexual differentiation and oxidative stress response in eukaryotes and the role of RNA binding proteins in the adaptation to environmental signals.
format	article
author	Ana M Matia-Gonzalez Jael Sotelo Miguel A Rodriguez-Gabriel
author_facet	Ana M Matia-Gonzalez Jael Sotelo Miguel A Rodriguez-Gabriel
author_sort	Ana M Matia-Gonzalez
title	The RNA binding protein Csx1 promotes sexual differentiation in Schizosaccharomyces pombe.
title_short	The RNA binding protein Csx1 promotes sexual differentiation in Schizosaccharomyces pombe.
title_full	The RNA binding protein Csx1 promotes sexual differentiation in Schizosaccharomyces pombe.
title_fullStr	The RNA binding protein Csx1 promotes sexual differentiation in Schizosaccharomyces pombe.
title_full_unstemmed	The RNA binding protein Csx1 promotes sexual differentiation in Schizosaccharomyces pombe.
title_sort	rna binding protein csx1 promotes sexual differentiation in schizosaccharomyces pombe.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/72ad1c3219a24fc28504b0348b55d364
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The RNA binding protein Csx1 promotes sexual differentiation in Schizosaccharomyces pombe.

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