Quantification of the Adhesion Strength of Candida albicans to Tooth Enamel

Caries is one of the most prevalent diseases worldwide, which is caused by the degradation of the tooth enamel surface. In earlier research the opportunistic pathogen Candida albicans has been associated with the formation of caries in children. Colonization of teeth by C...

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Autores principales:	Gubesh Gunaratnam, Johanna Dudek, Philipp Jung, Sören L. Becker, Karin Jacobs, Markus Bischoff, Matthias Hannig
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	single-cell force spectroscopy <i>Candida albicans</i> dental caries adhesion Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/72b1a217fb5e41c295ab5db149a6f574
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spelling	oai:doaj.org-article:72b1a217fb5e41c295ab5db149a6f5742021-11-25T18:24:17ZQuantification of the Adhesion Strength of <i>Candida albicans</i> to Tooth Enamel10.3390/microorganisms91122132076-2607https://doaj.org/article/72b1a217fb5e41c295ab5db149a6f5742021-10-01T00:00:00Zhttps://www.mdpi.com/2076-2607/9/11/2213https://doaj.org/toc/2076-2607Caries is one of the most prevalent diseases worldwide, which is caused by the degradation of the tooth enamel surface. In earlier research the opportunistic pathogen <i>Candida albicans</i> has been associated with the formation of caries in children. Colonization of teeth by <i>C. albicans</i> starts with the initial adhesion of individual yeast cells to the tooth enamel surface. In this study, we visualized the initial colonization of <i>C. albicans</i> yeast cells on pellicle-covered enamel by scanning electron microscopy. To quantitatively unravel the initial adhesion strength, we applied fluidic force microscopy-based single-cell force spectroscopy to examine the key adhesion parameters adhesion force, rupture length and de-adhesion work. We analyzed single saliva-treated or untreated yeast cells on tooth enamel specimens with or without salivary pellicle. Under all tested conditions, adhesion forces in the lower nanonewton range were determined. Furthermore, we have found that all adhesion parameters were enhanced on the pellicle-covered compared to the uncovered enamel. Our data suggest that initial adhesion occurs through a strong interaction between yeast cell wall-associated adhesins and the salivary pellicle. Future SCFS studies may show whether specific management of the salivary pellicle reduces the adhesion of <i>C. albicans</i> on teeth and thus contributes to caries prophylaxis.Gubesh GunaratnamJohanna DudekPhilipp JungSören L. BeckerKarin JacobsMarkus BischoffMatthias HannigMDPI AGarticlesingle-cell force spectroscopy<i>Candida albicans</i>dental cariesadhesionBiology (General)QH301-705.5ENMicroorganisms, Vol 9, Iss 2213, p 2213 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	single-cell force spectroscopy <i>Candida albicans</i> dental caries adhesion Biology (General) QH301-705.5
spellingShingle	single-cell force spectroscopy <i>Candida albicans</i> dental caries adhesion Biology (General) QH301-705.5 Gubesh Gunaratnam Johanna Dudek Philipp Jung Sören L. Becker Karin Jacobs Markus Bischoff Matthias Hannig Quantification of the Adhesion Strength of <i>Candida albicans</i> to Tooth Enamel
description	Caries is one of the most prevalent diseases worldwide, which is caused by the degradation of the tooth enamel surface. In earlier research the opportunistic pathogen <i>Candida albicans</i> has been associated with the formation of caries in children. Colonization of teeth by <i>C. albicans</i> starts with the initial adhesion of individual yeast cells to the tooth enamel surface. In this study, we visualized the initial colonization of <i>C. albicans</i> yeast cells on pellicle-covered enamel by scanning electron microscopy. To quantitatively unravel the initial adhesion strength, we applied fluidic force microscopy-based single-cell force spectroscopy to examine the key adhesion parameters adhesion force, rupture length and de-adhesion work. We analyzed single saliva-treated or untreated yeast cells on tooth enamel specimens with or without salivary pellicle. Under all tested conditions, adhesion forces in the lower nanonewton range were determined. Furthermore, we have found that all adhesion parameters were enhanced on the pellicle-covered compared to the uncovered enamel. Our data suggest that initial adhesion occurs through a strong interaction between yeast cell wall-associated adhesins and the salivary pellicle. Future SCFS studies may show whether specific management of the salivary pellicle reduces the adhesion of <i>C. albicans</i> on teeth and thus contributes to caries prophylaxis.
format	article
author	Gubesh Gunaratnam Johanna Dudek Philipp Jung Sören L. Becker Karin Jacobs Markus Bischoff Matthias Hannig
author_facet	Gubesh Gunaratnam Johanna Dudek Philipp Jung Sören L. Becker Karin Jacobs Markus Bischoff Matthias Hannig
author_sort	Gubesh Gunaratnam
title	Quantification of the Adhesion Strength of <i>Candida albicans</i> to Tooth Enamel
title_short	Quantification of the Adhesion Strength of <i>Candida albicans</i> to Tooth Enamel
title_full	Quantification of the Adhesion Strength of <i>Candida albicans</i> to Tooth Enamel
title_fullStr	Quantification of the Adhesion Strength of <i>Candida albicans</i> to Tooth Enamel
title_full_unstemmed	Quantification of the Adhesion Strength of <i>Candida albicans</i> to Tooth Enamel
title_sort	quantification of the adhesion strength of <i>candida albicans</i> to tooth enamel
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/72b1a217fb5e41c295ab5db149a6f574
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Quantification of the Adhesion Strength of <i>Candida albicans</i> to Tooth Enamel

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