Determination of Caffeic Acid in Ethanolic Extract of Spent Coffee Grounds by High-Performance Liquid Chromatography with UV Detection

We developed a method for determining the caffeic acid in spent coffee grounds. The spent coffee ground solution was prepared by blending 3 g spent coffee grounds with 60 mL ethanol/water (40/60 v/v) for 2 h on a hot plate magnetic stirrer (60 °C, 350 rpm). The mixture was filtered and the filtrate...

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Autores principales:	Michael Raharja Gani, Enade Perdana Istyastono
Formato:	article
Lenguaje:	EN
Publicado:	Department of Chemistry, Universitas Gadjah Mada 2021
Materias:	caffeic acid reversed phase high performance liquid chromatography spent coffee grounds Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/72b22df929b9403ea965b9f6663fae2d
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spelling	oai:doaj.org-article:72b22df929b9403ea965b9f6663fae2d2021-12-02T16:56:26ZDetermination of Caffeic Acid in Ethanolic Extract of Spent Coffee Grounds by High-Performance Liquid Chromatography with UV Detection1411-94202460-157810.22146/ijc.61462https://doaj.org/article/72b22df929b9403ea965b9f6663fae2d2021-08-01T00:00:00Zhttps://jurnal.ugm.ac.id/ijc/article/view/61462https://doaj.org/toc/1411-9420https://doaj.org/toc/2460-1578We developed a method for determining the caffeic acid in spent coffee grounds. The spent coffee ground solution was prepared by blending 3 g spent coffee grounds with 60 mL ethanol/water (40/60 v/v) for 2 h on a hot plate magnetic stirrer (60 °C, 350 rpm). The mixture was filtered and the filtrate was concentrated under vacuum (60 °C) to 5 mL. The method employed a reversed-phase high-performance liquid chromatography with a UV detector. We used a Phenomenex Luna column (250 × 4.6 mm; i.d., 5 µm) under isocratic elution, and the mobile phase was acetonitrile-methanol-aqueous formic acid (10:10:80 v/v), with a flow rate of 0.9 mL/min. Analysis was performed at 324 nm. The column temperature was set at 27 °C temperature. The results showed that this method was selective for quantifying the caffeic acid in spent coffee grounds with good linearity in the range of 1.31–17.07 μg/mL. The detection and quantitation limits were 0.28 and 0.84 μg/mL, respectively. The mean intraday and interday recoveries were 83.80–95.17% and 82.16–97.40%, respectively. Intraday and interday precision expressed as the relative standard deviation (RSD) were below 7.3%. There was 0.17% ± 0.006 w/w caffeic acid in the spent coffee grounds (RSD = 3.63%, n = 3).Michael Raharja GaniEnade Perdana IstyastonoDepartment of Chemistry, Universitas Gadjah Madaarticlecaffeic acidreversed phase high performance liquid chromatographyspent coffee groundsChemistryQD1-999ENIndonesian Journal of Chemistry, Vol 21, Iss 5, Pp 1281-1286 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	caffeic acid reversed phase high performance liquid chromatography spent coffee grounds Chemistry QD1-999
spellingShingle	caffeic acid reversed phase high performance liquid chromatography spent coffee grounds Chemistry QD1-999 Michael Raharja Gani Enade Perdana Istyastono Determination of Caffeic Acid in Ethanolic Extract of Spent Coffee Grounds by High-Performance Liquid Chromatography with UV Detection
description	We developed a method for determining the caffeic acid in spent coffee grounds. The spent coffee ground solution was prepared by blending 3 g spent coffee grounds with 60 mL ethanol/water (40/60 v/v) for 2 h on a hot plate magnetic stirrer (60 °C, 350 rpm). The mixture was filtered and the filtrate was concentrated under vacuum (60 °C) to 5 mL. The method employed a reversed-phase high-performance liquid chromatography with a UV detector. We used a Phenomenex Luna column (250 × 4.6 mm; i.d., 5 µm) under isocratic elution, and the mobile phase was acetonitrile-methanol-aqueous formic acid (10:10:80 v/v), with a flow rate of 0.9 mL/min. Analysis was performed at 324 nm. The column temperature was set at 27 °C temperature. The results showed that this method was selective for quantifying the caffeic acid in spent coffee grounds with good linearity in the range of 1.31–17.07 μg/mL. The detection and quantitation limits were 0.28 and 0.84 μg/mL, respectively. The mean intraday and interday recoveries were 83.80–95.17% and 82.16–97.40%, respectively. Intraday and interday precision expressed as the relative standard deviation (RSD) were below 7.3%. There was 0.17% ± 0.006 w/w caffeic acid in the spent coffee grounds (RSD = 3.63%, n = 3).
format	article
author	Michael Raharja Gani Enade Perdana Istyastono
author_facet	Michael Raharja Gani Enade Perdana Istyastono
author_sort	Michael Raharja Gani
title	Determination of Caffeic Acid in Ethanolic Extract of Spent Coffee Grounds by High-Performance Liquid Chromatography with UV Detection
title_short	Determination of Caffeic Acid in Ethanolic Extract of Spent Coffee Grounds by High-Performance Liquid Chromatography with UV Detection
title_full	Determination of Caffeic Acid in Ethanolic Extract of Spent Coffee Grounds by High-Performance Liquid Chromatography with UV Detection
title_fullStr	Determination of Caffeic Acid in Ethanolic Extract of Spent Coffee Grounds by High-Performance Liquid Chromatography with UV Detection
title_full_unstemmed	Determination of Caffeic Acid in Ethanolic Extract of Spent Coffee Grounds by High-Performance Liquid Chromatography with UV Detection
title_sort	determination of caffeic acid in ethanolic extract of spent coffee grounds by high-performance liquid chromatography with uv detection
publisher	Department of Chemistry, Universitas Gadjah Mada
publishDate	2021
url	https://doaj.org/article/72b22df929b9403ea965b9f6663fae2d
work_keys_str_mv	AT michaelraharjagani determinationofcaffeicacidinethanolicextractofspentcoffeegroundsbyhighperformanceliquidchromatographywithuvdetection AT enadeperdanaistyastono determinationofcaffeicacidinethanolicextractofspentcoffeegroundsbyhighperformanceliquidchromatographywithuvdetection
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Determination of Caffeic Acid in Ethanolic Extract of Spent Coffee Grounds by High-Performance Liquid Chromatography with UV Detection

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