Astaxanthin-Producing Microalgae Identification Using 18S rRNA : Isolates from Bangkalan Mangrove Waters and Sowan Tuban Northern Waters, East Java, Indonesia

Microalgae are a group of micro-sized photosynthetic organisms that range from prokaryotic cyanobacteria to eukaryotic algae. Microalgae are widely used as a source of natural food, cosmetic ingredients, food ingredients, and a source of pigments. This study aims to identify species of four microal...

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Autores principales:	Dini Ermavitalini, Siska Yulia Rukhmana, Thalita Meidina, Leonardo Pascalis Dimas Cahyo Baskoro, Triono Bagus Saputro, Ni’matuzahroh Ni’matuzahroh, Hery Purnobasuki
Formato:	article
Lenguaje:	EN
Publicado:	Universitas Gadjah Mada 2021
Materias:	astaxanthin, bangkalan mangrove waters, chlorella sp., picochlorum maculatum sowan tuban northern waters Agriculture (General) S1-972 Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/72b2a98484d24de68e0c5c6548086646
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spelling	oai:doaj.org-article:72b2a98484d24de68e0c5c65480866462021-11-08T08:40:57ZAstaxanthin-Producing Microalgae Identification Using 18S rRNA : Isolates from Bangkalan Mangrove Waters and Sowan Tuban Northern Waters, East Java, Indonesia2540-95732540-958110.22146/jtbb.64882https://doaj.org/article/72b2a98484d24de68e0c5c65480866462021-10-01T00:00:00Zhttps://jurnal.ugm.ac.id/jtbb/article/view/64882https://doaj.org/toc/2540-9573https://doaj.org/toc/2540-9581Microalgae are a group of micro-sized photosynthetic organisms that range from prokaryotic cyanobacteria to eukaryotic algae. Microalgae are widely used as a source of natural food, cosmetic ingredients, food ingredients, and a source of pigments. This study aims to identify species of four microalgae isolates named B1, B2, B3, and S2 from Bangkalan Mangrove Waters and Sowan Tuban Northern Waters, and to determine their astaxanthin pigment concentration under 1 M NaCl. Species identification was carried out through a molecular approach by utilization of an 18S rRNA gene marker. A quantitative test of astaxanthin concentration was carried out by spectrophotometric analysis. Molecular identification results show that isolates B1 and B3 are closely related to Chlorella sp., while isolates B2 and S2 are closely related to Picochlorum maculatum. Moreover, under salinity stress condition of 1 M NaCl shown a significant decrement of astaxanthin production compared to the control treatment. At 1 M NaCl, the astaxanthin content of isolate B1 was 4x10-5 mgL-1, isolate B2 was 2x10-5 mgL-1, isolate B3 was 1x10-5 mgL-1, and isolate S2 was 6x10-6 mgL-1. All in all, isolate S2 has the highest astaxanthin among the other isolates at normal conditions, while under salt stress regime, isolate B1 shown to be the best source for astaxanthin.Dini ErmavitaliniSiska Yulia RukhmanaThalita MeidinaLeonardo Pascalis Dimas Cahyo BaskoroTriono Bagus SaputroNi’matuzahroh Ni’matuzahrohHery PurnobasukiUniversitas Gadjah Madaarticleastaxanthin, bangkalan mangrove waters, chlorella sp., picochlorum maculatum sowan tuban northern watersAgriculture (General)S1-972Plant cultureSB1-1110ENJournal of Tropical Biodiversity and Biotechnology, Vol 6, Iss 3 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	astaxanthin, bangkalan mangrove waters, chlorella sp., picochlorum maculatum sowan tuban northern waters Agriculture (General) S1-972 Plant culture SB1-1110
spellingShingle	astaxanthin, bangkalan mangrove waters, chlorella sp., picochlorum maculatum sowan tuban northern waters Agriculture (General) S1-972 Plant culture SB1-1110 Dini Ermavitalini Siska Yulia Rukhmana Thalita Meidina Leonardo Pascalis Dimas Cahyo Baskoro Triono Bagus Saputro Ni’matuzahroh Ni’matuzahroh Hery Purnobasuki Astaxanthin-Producing Microalgae Identification Using 18S rRNA : Isolates from Bangkalan Mangrove Waters and Sowan Tuban Northern Waters, East Java, Indonesia
description	Microalgae are a group of micro-sized photosynthetic organisms that range from prokaryotic cyanobacteria to eukaryotic algae. Microalgae are widely used as a source of natural food, cosmetic ingredients, food ingredients, and a source of pigments. This study aims to identify species of four microalgae isolates named B1, B2, B3, and S2 from Bangkalan Mangrove Waters and Sowan Tuban Northern Waters, and to determine their astaxanthin pigment concentration under 1 M NaCl. Species identification was carried out through a molecular approach by utilization of an 18S rRNA gene marker. A quantitative test of astaxanthin concentration was carried out by spectrophotometric analysis. Molecular identification results show that isolates B1 and B3 are closely related to Chlorella sp., while isolates B2 and S2 are closely related to Picochlorum maculatum. Moreover, under salinity stress condition of 1 M NaCl shown a significant decrement of astaxanthin production compared to the control treatment. At 1 M NaCl, the astaxanthin content of isolate B1 was 4x10-5 mgL-1, isolate B2 was 2x10-5 mgL-1, isolate B3 was 1x10-5 mgL-1, and isolate S2 was 6x10-6 mgL-1. All in all, isolate S2 has the highest astaxanthin among the other isolates at normal conditions, while under salt stress regime, isolate B1 shown to be the best source for astaxanthin.
format	article
author	Dini Ermavitalini Siska Yulia Rukhmana Thalita Meidina Leonardo Pascalis Dimas Cahyo Baskoro Triono Bagus Saputro Ni’matuzahroh Ni’matuzahroh Hery Purnobasuki
author_facet	Dini Ermavitalini Siska Yulia Rukhmana Thalita Meidina Leonardo Pascalis Dimas Cahyo Baskoro Triono Bagus Saputro Ni’matuzahroh Ni’matuzahroh Hery Purnobasuki
author_sort	Dini Ermavitalini
title	Astaxanthin-Producing Microalgae Identification Using 18S rRNA : Isolates from Bangkalan Mangrove Waters and Sowan Tuban Northern Waters, East Java, Indonesia
title_short	Astaxanthin-Producing Microalgae Identification Using 18S rRNA : Isolates from Bangkalan Mangrove Waters and Sowan Tuban Northern Waters, East Java, Indonesia
title_full	Astaxanthin-Producing Microalgae Identification Using 18S rRNA : Isolates from Bangkalan Mangrove Waters and Sowan Tuban Northern Waters, East Java, Indonesia
title_fullStr	Astaxanthin-Producing Microalgae Identification Using 18S rRNA : Isolates from Bangkalan Mangrove Waters and Sowan Tuban Northern Waters, East Java, Indonesia
title_full_unstemmed	Astaxanthin-Producing Microalgae Identification Using 18S rRNA : Isolates from Bangkalan Mangrove Waters and Sowan Tuban Northern Waters, East Java, Indonesia
title_sort	astaxanthin-producing microalgae identification using 18s rrna : isolates from bangkalan mangrove waters and sowan tuban northern waters, east java, indonesia
publisher	Universitas Gadjah Mada
publishDate	2021
url	https://doaj.org/article/72b2a98484d24de68e0c5c6548086646
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Astaxanthin-Producing Microalgae Identification Using 18S rRNA : Isolates from Bangkalan Mangrove Waters and Sowan Tuban Northern Waters, East Java, Indonesia

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