Hepatic Response of Magnesium-Restricted Wild Type Mice

Magnesium-deficiency is implicated in many metabolic disorders, e.g., type 2 diabetes and metabolic syndrome, representing risk factors for non-alcoholic fatty liver disease (NAFLD). This study aims to investigate the contribution of magnesium-restriction to the development of NAFLD. Magnesium-defic...

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Autores principales:	Vera H. Fengler, Tanja Macheiner, Walter Goessler, Maria Ratzer, Johannes Haybaeck, Karine Sargsyan
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	magnesium-restriction magnesium-deficiency experimental mouse model non-alcoholic fatty liver disease hepatic inflammation hepatic steatosis Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/72b6711ff27e495a9d81ad6ea3dc0f7f
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spelling	oai:doaj.org-article:72b6711ff27e495a9d81ad6ea3dc0f7f2021-11-25T18:20:44ZHepatic Response of Magnesium-Restricted Wild Type Mice10.3390/metabo111107622218-1989https://doaj.org/article/72b6711ff27e495a9d81ad6ea3dc0f7f2021-11-01T00:00:00Zhttps://www.mdpi.com/2218-1989/11/11/762https://doaj.org/toc/2218-1989Magnesium-deficiency is implicated in many metabolic disorders, e.g., type 2 diabetes and metabolic syndrome, representing risk factors for non-alcoholic fatty liver disease (NAFLD). This study aims to investigate the contribution of magnesium-restriction to the development of NAFLD. Magnesium-deficiency was induced in C57BL/6 mice by feeding a magnesium-deficient-diet. Metabolic markers as well as markers of inflammation and liver function were assessed. Furthermore, liver tissue was examined histopathologically and compared with specimens from high-fat-diet fed and control mice. Finally, the hepatic inflammatory response was quantified by determining hepatic IL-6, TNFα, and MCP-1. Magnesium-restriction resulted in at least a 2-fold significant reduction of serum magnesium levels compared to the high-fat-diet fed and control mice, whereas the hepatic magnesium content was decreased due to high-fat-diet feeding. No changes in metabolic markers in magnesium-restricted mice were observed, while the cholesterol content was elevated in high-fat-diet fed mice. Magnesium-restricted mice additionally featured inflammation and enlarged hepatocytes in liver histology. Furthermore, magnesium-restricted and high-fat-diet fed mice exhibited elevated hepatic TNFα levels compared to control mice. Accordingly, our data suggest that magnesium is involved in hepatic inflammatory processes and hepatocyte enlargement, key histological features of human NAFLD, and may therefore contribute to development and progression of the disease.Vera H. FenglerTanja MacheinerWalter GoesslerMaria RatzerJohannes HaybaeckKarine SargsyanMDPI AGarticlemagnesium-restrictionmagnesium-deficiencyexperimental mouse modelnon-alcoholic fatty liver diseasehepatic inflammationhepatic steatosisMicrobiologyQR1-502ENMetabolites, Vol 11, Iss 762, p 762 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	magnesium-restriction magnesium-deficiency experimental mouse model non-alcoholic fatty liver disease hepatic inflammation hepatic steatosis Microbiology QR1-502
spellingShingle	magnesium-restriction magnesium-deficiency experimental mouse model non-alcoholic fatty liver disease hepatic inflammation hepatic steatosis Microbiology QR1-502 Vera H. Fengler Tanja Macheiner Walter Goessler Maria Ratzer Johannes Haybaeck Karine Sargsyan Hepatic Response of Magnesium-Restricted Wild Type Mice
description	Magnesium-deficiency is implicated in many metabolic disorders, e.g., type 2 diabetes and metabolic syndrome, representing risk factors for non-alcoholic fatty liver disease (NAFLD). This study aims to investigate the contribution of magnesium-restriction to the development of NAFLD. Magnesium-deficiency was induced in C57BL/6 mice by feeding a magnesium-deficient-diet. Metabolic markers as well as markers of inflammation and liver function were assessed. Furthermore, liver tissue was examined histopathologically and compared with specimens from high-fat-diet fed and control mice. Finally, the hepatic inflammatory response was quantified by determining hepatic IL-6, TNFα, and MCP-1. Magnesium-restriction resulted in at least a 2-fold significant reduction of serum magnesium levels compared to the high-fat-diet fed and control mice, whereas the hepatic magnesium content was decreased due to high-fat-diet feeding. No changes in metabolic markers in magnesium-restricted mice were observed, while the cholesterol content was elevated in high-fat-diet fed mice. Magnesium-restricted mice additionally featured inflammation and enlarged hepatocytes in liver histology. Furthermore, magnesium-restricted and high-fat-diet fed mice exhibited elevated hepatic TNFα levels compared to control mice. Accordingly, our data suggest that magnesium is involved in hepatic inflammatory processes and hepatocyte enlargement, key histological features of human NAFLD, and may therefore contribute to development and progression of the disease.
format	article
author	Vera H. Fengler Tanja Macheiner Walter Goessler Maria Ratzer Johannes Haybaeck Karine Sargsyan
author_facet	Vera H. Fengler Tanja Macheiner Walter Goessler Maria Ratzer Johannes Haybaeck Karine Sargsyan
author_sort	Vera H. Fengler
title	Hepatic Response of Magnesium-Restricted Wild Type Mice
title_short	Hepatic Response of Magnesium-Restricted Wild Type Mice
title_full	Hepatic Response of Magnesium-Restricted Wild Type Mice
title_fullStr	Hepatic Response of Magnesium-Restricted Wild Type Mice
title_full_unstemmed	Hepatic Response of Magnesium-Restricted Wild Type Mice
title_sort	hepatic response of magnesium-restricted wild type mice
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/72b6711ff27e495a9d81ad6ea3dc0f7f
work_keys_str_mv	AT verahfengler hepaticresponseofmagnesiumrestrictedwildtypemice AT tanjamacheiner hepaticresponseofmagnesiumrestrictedwildtypemice AT waltergoessler hepaticresponseofmagnesiumrestrictedwildtypemice AT mariaratzer hepaticresponseofmagnesiumrestrictedwildtypemice AT johanneshaybaeck hepaticresponseofmagnesiumrestrictedwildtypemice AT karinesargsyan hepaticresponseofmagnesiumrestrictedwildtypemice
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Hepatic Response of Magnesium-Restricted Wild Type Mice

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