Selection of reference genes for gene expression analysis in Liriodendron hybrids’ somatic embryogenesis and germinative tissues

Abstract The differential expression of genes is crucial for plant somatic embryogenesis (SE), and the accurate quantification of gene expression levels relies on choosing appropriate reference genes. To select the most suitable reference genes for SE studies, 10 commonly used reference genes were e...

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Autores principales:	Tingting Li, Weigao Yuan, Shuai Qiu, Jisen Shi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/72b6f273852a4f9bbe361d8cd3185539
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spelling	oai:doaj.org-article:72b6f273852a4f9bbe361d8cd31855392021-12-02T13:35:04ZSelection of reference genes for gene expression analysis in Liriodendron hybrids’ somatic embryogenesis and germinative tissues10.1038/s41598-021-84518-w2045-2322https://doaj.org/article/72b6f273852a4f9bbe361d8cd31855392021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-84518-whttps://doaj.org/toc/2045-2322Abstract The differential expression of genes is crucial for plant somatic embryogenesis (SE), and the accurate quantification of gene expression levels relies on choosing appropriate reference genes. To select the most suitable reference genes for SE studies, 10 commonly used reference genes were examined in synchronized somatic embryogenic and subsequent germinative cultures of Liriodendron hybrids by using quantitative real-time reverse transcription PCR. Four popular normalization algorithms: geNorm, NormFinder, Bestkeeper and Delta-Ct were used to select and validate the suitable reference genes. The results showed that elongation factor 1-gamma, histone H1 linker protein, glyceraldehyde-3-phosphate dehydrogenase and α-tubulin were suitable for SE tissues, while elongation factor 1-gamma and actin were best for the germinative organ tissues. Our work will benefit future studies of gene expression and functional analyses of SE in Liriodendron hybrids. It is also serves as a guide of reference gene selection in early embryonic gene expression analyses for other woody plant species.Tingting LiWeigao YuanShuai QiuJisen ShiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Tingting Li Weigao Yuan Shuai Qiu Jisen Shi Selection of reference genes for gene expression analysis in Liriodendron hybrids’ somatic embryogenesis and germinative tissues
description	Abstract The differential expression of genes is crucial for plant somatic embryogenesis (SE), and the accurate quantification of gene expression levels relies on choosing appropriate reference genes. To select the most suitable reference genes for SE studies, 10 commonly used reference genes were examined in synchronized somatic embryogenic and subsequent germinative cultures of Liriodendron hybrids by using quantitative real-time reverse transcription PCR. Four popular normalization algorithms: geNorm, NormFinder, Bestkeeper and Delta-Ct were used to select and validate the suitable reference genes. The results showed that elongation factor 1-gamma, histone H1 linker protein, glyceraldehyde-3-phosphate dehydrogenase and α-tubulin were suitable for SE tissues, while elongation factor 1-gamma and actin were best for the germinative organ tissues. Our work will benefit future studies of gene expression and functional analyses of SE in Liriodendron hybrids. It is also serves as a guide of reference gene selection in early embryonic gene expression analyses for other woody plant species.
format	article
author	Tingting Li Weigao Yuan Shuai Qiu Jisen Shi
author_facet	Tingting Li Weigao Yuan Shuai Qiu Jisen Shi
author_sort	Tingting Li
title	Selection of reference genes for gene expression analysis in Liriodendron hybrids’ somatic embryogenesis and germinative tissues
title_short	Selection of reference genes for gene expression analysis in Liriodendron hybrids’ somatic embryogenesis and germinative tissues
title_full	Selection of reference genes for gene expression analysis in Liriodendron hybrids’ somatic embryogenesis and germinative tissues
title_fullStr	Selection of reference genes for gene expression analysis in Liriodendron hybrids’ somatic embryogenesis and germinative tissues
title_full_unstemmed	Selection of reference genes for gene expression analysis in Liriodendron hybrids’ somatic embryogenesis and germinative tissues
title_sort	selection of reference genes for gene expression analysis in liriodendron hybrids’ somatic embryogenesis and germinative tissues
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/72b6f273852a4f9bbe361d8cd3185539
work_keys_str_mv	AT tingtingli selectionofreferencegenesforgeneexpressionanalysisinliriodendronhybridssomaticembryogenesisandgerminativetissues AT weigaoyuan selectionofreferencegenesforgeneexpressionanalysisinliriodendronhybridssomaticembryogenesisandgerminativetissues AT shuaiqiu selectionofreferencegenesforgeneexpressionanalysisinliriodendronhybridssomaticembryogenesisandgerminativetissues AT jisenshi selectionofreferencegenesforgeneexpressionanalysisinliriodendronhybridssomaticembryogenesisandgerminativetissues
_version_	1718392680914878464

Selection of reference genes for gene expression analysis in Liriodendron hybrids’ somatic embryogenesis and germinative tissues

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