Determination of CYP1A2 Phenotype after Oral Administration of Caffeine in a Sample of Healthy Volunteer from Mazandaran Province

BACKGROUND AND OBJECTIVE: Assay of caffeine (CA) plasma concentration can help to find out the activity level of hepatic microsomal cytochrome oxidase 1A2 (CYP1A2). This study was conducted to evaluate the phenotype of activity of CYP2A1 in a sample of Iranian volunteers based on salivary concentrat...

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Autores principales:	S.SH Rostam kolaee, S GharooeeAhangar, MT Kazemi, MR Shiran, AA Moghadamnia
Formato:	article
Lenguaje:	EN FA
Publicado:	Babol University of Medical Sciences 2012
Materias:	cyp2a1 caffeine elimination half time hplc saliva Medicine R Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/72bb954fc00f4e1189ef874b06435566
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spelling	oai:doaj.org-article:72bb954fc00f4e1189ef874b064355662021-11-10T08:53:33ZDetermination of CYP1A2 Phenotype after Oral Administration of Caffeine in a Sample of Healthy Volunteer from Mazandaran Province1561-41072251-7170https://doaj.org/article/72bb954fc00f4e1189ef874b064355662012-11-01T00:00:00Zhttp://jbums.org/article-1-4234-en.htmlhttps://doaj.org/toc/1561-4107https://doaj.org/toc/2251-7170BACKGROUND AND OBJECTIVE: Assay of caffeine (CA) plasma concentration can help to find out the activity level of hepatic microsomal cytochrome oxidase 1A2 (CYP1A2). This study was conducted to evaluate the phenotype of activity of CYP2A1 in a sample of Iranian volunteers based on salivary concentration of CA as typical probe of CYP1A2.METHODS: Saliva concentration of CA in 100 Iranian healthy subjects from Mazandaran province (82 men and 18 women) was measured at 0, 0.5, 2, and 5 h after drinking a cup of coffee containing 50 mg caffeine using HPLC method. C8 analytical column and a mobile phase composed of methanol (60%) and water (40%) were used for the chromatographic separation. The peak was detected using a UV detector set at 210 nm.FINDINGS: The mean ± SD of age and body mass index were 24.5 ± 6.9 years (range: 18-55 years) and 23.45± 3.7 (range: 16.7-37.4), respectively. The CV was 3.2% and good linearity (R2= 0.997) was confirmed. The mean elimination half time of CA was 2.01 ± 0.124 h (1.98 h for females and 2.02 h for males) and elimination half-time of CA in above 30 years old (2.05 h) was higher than below 30 years old (2.00 h). The mean Cmax of CA was 0.16 ± 0.02 micg/ml and Kel was calculated 0.41 ± 0.016.CONCLUSION: A normal phenotype of CYP2A1 activity was observed in the Iranian participants in this study. The CYP2A1 activity is higher in elderly subjects than in young.S.SH Rostam kolaee,S GharooeeAhangarMT KazemiMR ShiranAA MoghadamniaBabol University of Medical Sciencesarticlecyp2a1caffeineelimination half timehplcsalivaMedicineRMedicine (General)R5-920ENFAMajallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Bābul, Vol 14, Iss 6, Pp 25-32 (2012)
institution	DOAJ
collection	DOAJ
language	EN FA
topic	cyp2a1 caffeine elimination half time hplc saliva Medicine R Medicine (General) R5-920
spellingShingle	cyp2a1 caffeine elimination half time hplc saliva Medicine R Medicine (General) R5-920 S.SH Rostam kolaee, S GharooeeAhangar MT Kazemi MR Shiran AA Moghadamnia Determination of CYP1A2 Phenotype after Oral Administration of Caffeine in a Sample of Healthy Volunteer from Mazandaran Province
description	BACKGROUND AND OBJECTIVE: Assay of caffeine (CA) plasma concentration can help to find out the activity level of hepatic microsomal cytochrome oxidase 1A2 (CYP1A2). This study was conducted to evaluate the phenotype of activity of CYP2A1 in a sample of Iranian volunteers based on salivary concentration of CA as typical probe of CYP1A2.METHODS: Saliva concentration of CA in 100 Iranian healthy subjects from Mazandaran province (82 men and 18 women) was measured at 0, 0.5, 2, and 5 h after drinking a cup of coffee containing 50 mg caffeine using HPLC method. C8 analytical column and a mobile phase composed of methanol (60%) and water (40%) were used for the chromatographic separation. The peak was detected using a UV detector set at 210 nm.FINDINGS: The mean ± SD of age and body mass index were 24.5 ± 6.9 years (range: 18-55 years) and 23.45± 3.7 (range: 16.7-37.4), respectively. The CV was 3.2% and good linearity (R2= 0.997) was confirmed. The mean elimination half time of CA was 2.01 ± 0.124 h (1.98 h for females and 2.02 h for males) and elimination half-time of CA in above 30 years old (2.05 h) was higher than below 30 years old (2.00 h). The mean Cmax of CA was 0.16 ± 0.02 micg/ml and Kel was calculated 0.41 ± 0.016.CONCLUSION: A normal phenotype of CYP2A1 activity was observed in the Iranian participants in this study. The CYP2A1 activity is higher in elderly subjects than in young.
format	article
author	S.SH Rostam kolaee, S GharooeeAhangar MT Kazemi MR Shiran AA Moghadamnia
author_facet	S.SH Rostam kolaee, S GharooeeAhangar MT Kazemi MR Shiran AA Moghadamnia
author_sort	S.SH Rostam kolaee,
title	Determination of CYP1A2 Phenotype after Oral Administration of Caffeine in a Sample of Healthy Volunteer from Mazandaran Province
title_short	Determination of CYP1A2 Phenotype after Oral Administration of Caffeine in a Sample of Healthy Volunteer from Mazandaran Province
title_full	Determination of CYP1A2 Phenotype after Oral Administration of Caffeine in a Sample of Healthy Volunteer from Mazandaran Province
title_fullStr	Determination of CYP1A2 Phenotype after Oral Administration of Caffeine in a Sample of Healthy Volunteer from Mazandaran Province
title_full_unstemmed	Determination of CYP1A2 Phenotype after Oral Administration of Caffeine in a Sample of Healthy Volunteer from Mazandaran Province
title_sort	determination of cyp1a2 phenotype after oral administration of caffeine in a sample of healthy volunteer from mazandaran province
publisher	Babol University of Medical Sciences
publishDate	2012
url	https://doaj.org/article/72bb954fc00f4e1189ef874b06435566
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Determination of CYP1A2 Phenotype after Oral Administration of Caffeine in a Sample of Healthy Volunteer from Mazandaran Province

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