Nuclei multiplexing with barcoded antibodies for single-nucleus genomics

Single-nucleus RNA-seq enables interrogation of complex tissues but is limited due to batch effects and processing costs. Here the authors use barcoded antibodies against the nuclear pore complex to label nuclei from distinct samples, and develop a computational tool to assign the sample of origin.

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Autores principales:	Jellert T. Gaublomme, Bo Li, Cristin McCabe, Abigail Knecht, Yiming Yang, Eugene Drokhlyansky, Nicholas Van Wittenberghe, Julia Waldman, Danielle Dionne, Lan Nguyen, Philip L. De Jager, Bertrand Yeung, Xinfang Zhao, Naomi Habib, Orit Rozenblatt-Rosen, Aviv Regev
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/72c120e589ce4b23822d48803ea3ebfb
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spelling	oai:doaj.org-article:72c120e589ce4b23822d48803ea3ebfb2021-12-02T17:02:11ZNuclei multiplexing with barcoded antibodies for single-nucleus genomics10.1038/s41467-019-10756-22041-1723https://doaj.org/article/72c120e589ce4b23822d48803ea3ebfb2019-07-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-10756-2https://doaj.org/toc/2041-1723Single-nucleus RNA-seq enables interrogation of complex tissues but is limited due to batch effects and processing costs. Here the authors use barcoded antibodies against the nuclear pore complex to label nuclei from distinct samples, and develop a computational tool to assign the sample of origin.Jellert T. GaublommeBo LiCristin McCabeAbigail KnechtYiming YangEugene DrokhlyanskyNicholas Van WittenbergheJulia WaldmanDanielle DionneLan NguyenPhilip L. De JagerBertrand YeungXinfang ZhaoNaomi HabibOrit Rozenblatt-RosenAviv RegevNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-8 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Jellert T. Gaublomme Bo Li Cristin McCabe Abigail Knecht Yiming Yang Eugene Drokhlyansky Nicholas Van Wittenberghe Julia Waldman Danielle Dionne Lan Nguyen Philip L. De Jager Bertrand Yeung Xinfang Zhao Naomi Habib Orit Rozenblatt-Rosen Aviv Regev Nuclei multiplexing with barcoded antibodies for single-nucleus genomics
description	Single-nucleus RNA-seq enables interrogation of complex tissues but is limited due to batch effects and processing costs. Here the authors use barcoded antibodies against the nuclear pore complex to label nuclei from distinct samples, and develop a computational tool to assign the sample of origin.
format	article
author	Jellert T. Gaublomme Bo Li Cristin McCabe Abigail Knecht Yiming Yang Eugene Drokhlyansky Nicholas Van Wittenberghe Julia Waldman Danielle Dionne Lan Nguyen Philip L. De Jager Bertrand Yeung Xinfang Zhao Naomi Habib Orit Rozenblatt-Rosen Aviv Regev
author_facet	Jellert T. Gaublomme Bo Li Cristin McCabe Abigail Knecht Yiming Yang Eugene Drokhlyansky Nicholas Van Wittenberghe Julia Waldman Danielle Dionne Lan Nguyen Philip L. De Jager Bertrand Yeung Xinfang Zhao Naomi Habib Orit Rozenblatt-Rosen Aviv Regev
author_sort	Jellert T. Gaublomme
title	Nuclei multiplexing with barcoded antibodies for single-nucleus genomics
title_short	Nuclei multiplexing with barcoded antibodies for single-nucleus genomics
title_full	Nuclei multiplexing with barcoded antibodies for single-nucleus genomics
title_fullStr	Nuclei multiplexing with barcoded antibodies for single-nucleus genomics
title_full_unstemmed	Nuclei multiplexing with barcoded antibodies for single-nucleus genomics
title_sort	nuclei multiplexing with barcoded antibodies for single-nucleus genomics
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/72c120e589ce4b23822d48803ea3ebfb
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Nuclei multiplexing with barcoded antibodies for single-nucleus genomics

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