Probing microtubules polarity in mitotic spindles in situ using Interferometric Second Harmonic Generation Microscopy

Abstract The polarity of microtubules is thought to be involved in spindle assembly, cytokinesis or active molecular transport. However, its exact role remains poorly understood, mainly because of the challenge to measure microtubule polarity in intact cells. We report here the use of fast Interfero...

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Autores principales:	S. Bancelin, C.-A. Couture, M. Pinsard, M. Rivard, P. Drapeau, F. Légaré
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/72c5fd078450436a9d3c757e7356cb1d
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spelling	oai:doaj.org-article:72c5fd078450436a9d3c757e7356cb1d2021-12-02T15:06:19ZProbing microtubules polarity in mitotic spindles in situ using Interferometric Second Harmonic Generation Microscopy10.1038/s41598-017-06648-42045-2322https://doaj.org/article/72c5fd078450436a9d3c757e7356cb1d2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06648-4https://doaj.org/toc/2045-2322Abstract The polarity of microtubules is thought to be involved in spindle assembly, cytokinesis or active molecular transport. However, its exact role remains poorly understood, mainly because of the challenge to measure microtubule polarity in intact cells. We report here the use of fast Interferometric Second Harmonic Generation microscopy to study the polarity of microtubules forming the mitotic spindles in a zebrafish embryo. This technique provides a powerful tool to study mitotic spindle formation and may be directly transferable for investigating the kinetics and function of microtubule polarity in other aspects of subcellular motility or in native tissues.S. BancelinC.-A. CoutureM. PinsardM. RivardP. DrapeauF. LégaréNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-8 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q S. Bancelin C.-A. Couture M. Pinsard M. Rivard P. Drapeau F. Légaré Probing microtubules polarity in mitotic spindles in situ using Interferometric Second Harmonic Generation Microscopy
description	Abstract The polarity of microtubules is thought to be involved in spindle assembly, cytokinesis or active molecular transport. However, its exact role remains poorly understood, mainly because of the challenge to measure microtubule polarity in intact cells. We report here the use of fast Interferometric Second Harmonic Generation microscopy to study the polarity of microtubules forming the mitotic spindles in a zebrafish embryo. This technique provides a powerful tool to study mitotic spindle formation and may be directly transferable for investigating the kinetics and function of microtubule polarity in other aspects of subcellular motility or in native tissues.
format	article
author	S. Bancelin C.-A. Couture M. Pinsard M. Rivard P. Drapeau F. Légaré
author_facet	S. Bancelin C.-A. Couture M. Pinsard M. Rivard P. Drapeau F. Légaré
author_sort	S. Bancelin
title	Probing microtubules polarity in mitotic spindles in situ using Interferometric Second Harmonic Generation Microscopy
title_short	Probing microtubules polarity in mitotic spindles in situ using Interferometric Second Harmonic Generation Microscopy
title_full	Probing microtubules polarity in mitotic spindles in situ using Interferometric Second Harmonic Generation Microscopy
title_fullStr	Probing microtubules polarity in mitotic spindles in situ using Interferometric Second Harmonic Generation Microscopy
title_full_unstemmed	Probing microtubules polarity in mitotic spindles in situ using Interferometric Second Harmonic Generation Microscopy
title_sort	probing microtubules polarity in mitotic spindles in situ using interferometric second harmonic generation microscopy
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/72c5fd078450436a9d3c757e7356cb1d
work_keys_str_mv	AT sbancelin probingmicrotubulespolarityinmitoticspindlesinsituusinginterferometricsecondharmonicgenerationmicroscopy AT cacouture probingmicrotubulespolarityinmitoticspindlesinsituusinginterferometricsecondharmonicgenerationmicroscopy AT mpinsard probingmicrotubulespolarityinmitoticspindlesinsituusinginterferometricsecondharmonicgenerationmicroscopy AT mrivard probingmicrotubulespolarityinmitoticspindlesinsituusinginterferometricsecondharmonicgenerationmicroscopy AT pdrapeau probingmicrotubulespolarityinmitoticspindlesinsituusinginterferometricsecondharmonicgenerationmicroscopy AT flegare probingmicrotubulespolarityinmitoticspindlesinsituusinginterferometricsecondharmonicgenerationmicroscopy
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Probing microtubules polarity in mitotic spindles in situ using Interferometric Second Harmonic Generation Microscopy

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