Neuronal delivery of nanoparticles via nerve fibres in the skin

Abstract Accessing the peripheral nervous system (PNS) by topically applied nanoparticles is a simple and novel approach with clinical applications in several PNS disorders. Skin is richly innervated by long peripheral axons that arise from cell bodies located distally within ganglia. In this study...

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Autores principales:	Neeraj Katiyar, Gayathri Raju, Pallavi Madhusudanan, Vignesh Gopalakrishnan-Prema, Sahadev A. Shankarappa
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/72cc51d694b2454380c8740a821ad996
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spelling	oai:doaj.org-article:72cc51d694b2454380c8740a821ad9962021-12-02T14:16:49ZNeuronal delivery of nanoparticles via nerve fibres in the skin10.1038/s41598-021-81995-x2045-2322https://doaj.org/article/72cc51d694b2454380c8740a821ad9962021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-81995-xhttps://doaj.org/toc/2045-2322Abstract Accessing the peripheral nervous system (PNS) by topically applied nanoparticles is a simple and novel approach with clinical applications in several PNS disorders. Skin is richly innervated by long peripheral axons that arise from cell bodies located distally within ganglia. In this study we attempt to target dorsal root ganglia (DRG) neurons, via their axons by topical application of lectin-functionalized gold nanoparticles (IB4-AuNP). In vitro, 140.2 ± 1.9 nm IB4-AuNP were found to bind both axons and cell bodies of DRG neurons, and AuNP applied at the axonal terminals were found to translocate to the cell bodies. Topical application of IB4-AuNP on rat hind-paw resulted in accumulation of three to fourfold higher AuNP in lumbar DRG than in contralateral control DRGs. Results from this study clearly suggest that topically applied nanoparticles with neurotropic targeting ligands can be utilized for delivering nanoparticles to neuronal cell bodies via axonal transport mechanisms.Neeraj KatiyarGayathri RajuPallavi MadhusudananVignesh Gopalakrishnan-PremaSahadev A. ShankarappaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Neeraj Katiyar Gayathri Raju Pallavi Madhusudanan Vignesh Gopalakrishnan-Prema Sahadev A. Shankarappa Neuronal delivery of nanoparticles via nerve fibres in the skin
description	Abstract Accessing the peripheral nervous system (PNS) by topically applied nanoparticles is a simple and novel approach with clinical applications in several PNS disorders. Skin is richly innervated by long peripheral axons that arise from cell bodies located distally within ganglia. In this study we attempt to target dorsal root ganglia (DRG) neurons, via their axons by topical application of lectin-functionalized gold nanoparticles (IB4-AuNP). In vitro, 140.2 ± 1.9 nm IB4-AuNP were found to bind both axons and cell bodies of DRG neurons, and AuNP applied at the axonal terminals were found to translocate to the cell bodies. Topical application of IB4-AuNP on rat hind-paw resulted in accumulation of three to fourfold higher AuNP in lumbar DRG than in contralateral control DRGs. Results from this study clearly suggest that topically applied nanoparticles with neurotropic targeting ligands can be utilized for delivering nanoparticles to neuronal cell bodies via axonal transport mechanisms.
format	article
author	Neeraj Katiyar Gayathri Raju Pallavi Madhusudanan Vignesh Gopalakrishnan-Prema Sahadev A. Shankarappa
author_facet	Neeraj Katiyar Gayathri Raju Pallavi Madhusudanan Vignesh Gopalakrishnan-Prema Sahadev A. Shankarappa
author_sort	Neeraj Katiyar
title	Neuronal delivery of nanoparticles via nerve fibres in the skin
title_short	Neuronal delivery of nanoparticles via nerve fibres in the skin
title_full	Neuronal delivery of nanoparticles via nerve fibres in the skin
title_fullStr	Neuronal delivery of nanoparticles via nerve fibres in the skin
title_full_unstemmed	Neuronal delivery of nanoparticles via nerve fibres in the skin
title_sort	neuronal delivery of nanoparticles via nerve fibres in the skin
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/72cc51d694b2454380c8740a821ad996
work_keys_str_mv	AT neerajkatiyar neuronaldeliveryofnanoparticlesvianervefibresintheskin AT gayathriraju neuronaldeliveryofnanoparticlesvianervefibresintheskin AT pallavimadhusudanan neuronaldeliveryofnanoparticlesvianervefibresintheskin AT vigneshgopalakrishnanprema neuronaldeliveryofnanoparticlesvianervefibresintheskin AT sahadevashankarappa neuronaldeliveryofnanoparticlesvianervefibresintheskin
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Neuronal delivery of nanoparticles via nerve fibres in the skin

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